Imperial College London
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ProfessorPaulKellam

Faculty of Medicine, Department of Infectious Disease

Professor of Virus Genomics
 
 
 
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Contact

 

p.kellam

 
 
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Location

 

460Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Benfield:2019:10.1101/737841,
author = {Benfield, C and MacKenzie, F and Ritzefeld, M and Mazzon, M and Weston, S and Tate, E and Han, Teo B and Smith, S and Kellam, P and Holmes, E and Marsh, M},
doi = {10.1101/737841},
publisher = {bioRxiv},
title = {Comparative analysis reveals adaptive evolution of bat IFITMs and a novel antiviral determinant},
url = {http://dx.doi.org/10.1101/737841},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - ABSTRACT Host interferon-induced transmembrane proteins (IFITMs) are broad-spectrum antiviral restriction factors. Of these, IFITM3 potently inhibits viruses that enter cells through acidic endosomes, many of which are zoonotic and emerging viruses with bats (order Chiroptera) as natural hosts. We previously demonstrated that microbat IFITM3 is antiviral. Here we show that bat IFITMs are characterized by strong adaptive evolution and identify a highly variable and functionally important site - codon 70 - within the conserved CD225 domain of IFITMs. Mutation of this residue in microbat IFITM3 impairs restriction of four different virus families that enter cells via endosomes. This mutant shows altered subcellular localization and reduced S-palmitoylation, a phenotype copied by mutation of conserved cysteine residues in microbat IFITM3. Furthermore, we show that microbat IFITM3 is S-palmitoylated on cysteine residues C71, C72 and C105, mutation of each cysteine residue individually impairs virus restriction, and a triple C71-C72-C105 mutant loses all restriction, concomitant with subcellular re-localization of microbat IFITM3 to Golgi-associated sites. Thus, we propose that S-palmitoylation is critical for Chiropteran IFITM3 function and identify a key molecular determinant of IFITM3 S-palmitoylation.
AU  - Benfield,C
AU  - MacKenzie,F
AU  - Ritzefeld,M
AU  - Mazzon,M
AU  - Weston,S
AU  - Tate,E
AU  - Han,Teo B
AU  - Smith,S
AU  - Kellam,P
AU  - Holmes,E
AU  - Marsh,M
DO  - 10.1101/737841
PB  - bioRxiv
PY  - 2019///
TI  - Comparative analysis reveals adaptive evolution of bat IFITMs and a novel antiviral determinant
UR  - http://dx.doi.org/10.1101/737841
UR  - https://www.biorxiv.org/content/10.1101/737841v1
UR  - http://hdl.handle.net/10044/1/81517
ER  -
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