Publications
139 results found
Martin KM, Cooper WN, Metcalfe JC, et al., 2000, Mouse BTEB3, a new member of the basic transcription element binding protein (BTEB) family, activates expression from GC-rich minimal promoter regions, BIOCHEMICAL JOURNAL, Vol: 345, Pages: 529-533, ISSN: 0264-6021
- Author Web Link
- Cite
- Citations: 33
Grainger DJ, Heathcote K, Chiano M, et al., 1999, Genetic control of the circulating concentration of transforming growth factor type β1, HUMAN MOLECULAR GENETICS, Vol: 8, Pages: 93-97, ISSN: 0964-6906
- Author Web Link
- Cite
- Citations: 648
Russell MW, Kemp P, Wang LH, et al., 1998, Molecular cloning of the human HAND2 gene, BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, Vol: 1443, Pages: 393-399, ISSN: 0167-4781
- Author Web Link
- Cite
- Citations: 18
Syrris P, Carter ND, Metcalfe JC, et al., 1998, Transforming growth factor-β gene polymorphisms and coronary artery disease, CLINICAL SCIENCE, Vol: 95, Pages: 659-667, ISSN: 0143-5221
- Author Web Link
- Cite
- Citations: 128
Öklü R, Hesketh TR, Metcalfe JC, et al., 1998, Expression of alternatively spliced human latent transforming growth factor β binding protein-1, FEBS LETTERS, Vol: 435, Pages: 143-148, ISSN: 1873-3468
- Author Web Link
- Cite
- Citations: 16
Horner A, Kemp P, Summers C, et al., 1998, Expression and distribution of transforming growth factor-β isoforms and their signaling receptors in growing human bone, BONE, Vol: 23, Pages: 95-102, ISSN: 8756-3282
- Author Web Link
- Cite
- Citations: 109
Öklü R, Metcalfe JC, Hesketh TR, et al., 1998, Loss of a consensus heparin binding site by alternative splicing of latent transforming growth factor-β binding protein-1, FEBS LETTERS, Vol: 425, Pages: 281-285, ISSN: 1873-3468
- Author Web Link
- Cite
- Citations: 27
Horner A, Bord S, Kemp P, et al., 1996, Distribution of platelet-derived growth factor (PDGF) a chain mRNA, protein, and PDGF-alpha receptor in rapidly forming human bone, BONE, Vol: 19, Pages: 353-362, ISSN: 8756-3282
- Author Web Link
- Cite
- Citations: 55
KEMP PR, OSBOURN JK, GRAINGER DJ, et al., 1995, CLONING AND ANALYSIS OF THE PROMOTER REGION OF THE RAT SM22-ALPHA GENE, BIOCHEMICAL JOURNAL, Vol: 310, Pages: 1037-1043, ISSN: 0264-6021
- Author Web Link
- Cite
- Citations: 42
Kemp PR, Osbourn JK, Grainger DJ, et al., 1995, Cloning and analysis of the promoter region of the rat SM22 alpha gene., Biochem J, Vol: 310 ( Pt 3), Pages: 1037-1043, ISSN: 0264-6021
We have cloned and sequenced a 1.9 kb fragment of the 5'-upstream sequence of the smooth-muscle-specific gene SM22 alpha. The region cloned consisted of the SM22 alpha promoter, a 65 bp exon containing most of the 5'-untranslated region and 307 bp of the first intron. A 1.5 kb fragment at the 5' end of this sequence was able to drive the expression of a reporter chloramphenicol acetyltransferase (CAT) gene in both vascular smooth-muscle cells and Rat-1 fibroblasts. This promoter region did not contain a consensus TATAA box but contained the sequence TTTAAA 25 bp from the major start site identified by primer extension. Deletion analysis showed that a fragment of the promoter from +65 to -303 was more active in both cell types than the 1.5 kb fragment suggesting that there are silencer sequences in the region 5' to the core promoter. CAT activity was also observed with fragments containing bases +65 to -193 and +65 to -117 in smooth-muscle cells. In contrast with the smooth-muscle cells, no CAT activity was detected in Rat-1 fibroblasts with the smallest two fragments. The residual promoter activity in the smallest fragment of the SM22 alpha promoter tested suggested that, unlike the smooth-muscle alpha-actin promoter, transcription from the SM22 alpha promoter can occur in smooth-muscle cells in the absence of factors binding to CC(A/Trich)6GG (CArG box) or CANNTG (E box) motifs.
KEMP PR, METCALFE JC, GRAINGER DJ, 1995, ID - A DOMINANT-NEGATIVE REGULATOR OF SKELETAL-MUSCLE DIFFERENTIATION - IS NOT INVOLVED IN MATURATION OR DIFFERENTIATION OF VASCULAR SMOOTH-MUSCLE CELLS, FEBS LETTERS, Vol: 368, Pages: 81-86, ISSN: 0014-5793
- Author Web Link
- Cite
- Citations: 7
GRAINGER DJ, MOSEDALE DE, METCALFE JC, et al., 1995, ACTIVE AND ACID-ACTIVATABLE TGF-BETA IN HUMAN SERA, PLATELETS AND PLASMA, CLINICA CHIMICA ACTA, Vol: 235, Pages: 11-31, ISSN: 0009-8981
- Author Web Link
- Cite
- Citations: 82
GRAINGER DJ, KEMP BR, METCALFE JC, et al., 1995, THE SERUM CONCENTRATION OF ACTIVE TRANSFORMING GROWTH-FACTOR-BETA IS SEVERELY DEPRESSED IN ADVANCED ATHEROSCLEROSIS, NATURE MEDICINE, Vol: 1, Pages: 74-79, ISSN: 1078-8956
- Author Web Link
- Cite
- Citations: 362
GRAINGER DJ, KEMP PR, LIU AC, et al., 1994, ACTIVATION OF TRANSFORMING GROWTH-FACTOR-BETA IS INHIBITED IN TRANSGENIC APOLIPOPROTEIN(A) MICE, NATURE, Vol: 370, Pages: 460-462, ISSN: 0028-0836
- Author Web Link
- Cite
- Citations: 339
Grainger DJ, Kemp PR, Witchell CM, et al., 1994, Transforming growth factor beta decreases the rate of proliferation of rat vascular smooth muscle cells by extending the G2 phase of the cell cycle and delays the rise in cyclic AMP before entry into M phase., Biochem J, Vol: 299 ( Pt 1), Pages: 227-235, ISSN: 0264-6021
Transforming growth factor beta 1 (TGF-beta 1) decreased the rate of proliferation of rat aortic vascular smooth muscle cells (VSMCs) stimulated with serum showing a maximal effect at > 5 ng/ml (200 pM). However, it did not reduce the proportion of cells which passed through S phase (> 90%) and entry into S phase was delayed by less than 3 h. The proportion of cells passing through M phase (> 90%) was also unaffected, but entry into mitosis was delayed by approx. 24 h. This increase in cell cycle time was therefore due mainly to an increase in the G2 to mitotic metaphase period. Addition of TGF-beta 1 late in G1 or late in S phase failed to delay the onset of mitosis, but the presence of TGF-beta 1 between 0 and 12 h after the addition of serum to quiescent cells was sufficient to cause the maximal delay in mitosis of approx. 24 h. The role of cyclic AMP in the mechanism of the TGF-beta 1 effects on the cell cycle was examined. Entry into mitosis was preceded by a transient 2-fold increase in cyclic AMP concentration and TGF-beta 1 delayed both this increase in cyclic AMP and entry into mitosis to the same extent. Addition of forskolin or 8-(4-chlorophenylthio)-cyclic AMP to cells 30 h after stimulation with serum completely reversed the increase in duration of G2 in the presence of TGF-beta 1, suggesting that the rise in cyclic AMP levels which precedes mitosis might trigger entry of the VSMCs into M phase. Addition of forskolin late in S phase (26 h after stimulation with serum) advanced the entry of the cells into M phase and they divided prematurely. This effect was unaffected by the addition of cycloheximide with the forskolin; however, the effect of forskolin on cell division was completely inhibited when cycloheximide was added late in G1. TGF-beta 1 prevented the loss of smooth-muscle-specific myosin heavy chain (SM-MHC), which occurs in primary VSMC cultures in the presence or absence of serum, and the cells proliferated while maintaining a differe
GRAINGER DJ, KEMP PR, WITCHELL CM, et al., 1994, TRANSFORMING GROWTH-FACTOR-BETA DECREASES THE RATE OF PROLIFERATION OF RAT VASCULAR SMOOTH-MUSCLE CELLS BY EXTENDING THE G(2) PHASE OF THE CELL-CYCLE AND DELAYS THE RISE IN CYCLIC-AMP BEFORE ENTRY INTO M-PHASE, BIOCHEMICAL JOURNAL, Vol: 299, Pages: 227-235, ISSN: 0264-6021
- Author Web Link
- Cite
- Citations: 70
KEMP PR, SHACHARHILL Y, WEISSBERG PL, et al., 1993, INHIBITION OF PDGF BB STIMULATED DNA-SYNTHESIS IN RAT AORTIC VASCULAR SMOOTH-MUSCLE CELLS BY THE EXPRESSION OF A TRUNCATED PDGF-BETA RECEPTOR, FEBS LETTERS, Vol: 336, Pages: 119-123, ISSN: 0014-5793
- Author Web Link
- Cite
- Citations: 4
Kemp PR, Grainger DJ, Shanahan CM, et al., 1991, The Id gene is activated by serum but is not required for de-differentiation in rat vascular smooth muscle cells., Biochem J, Vol: 277 ( Pt 1), Pages: 285-288, ISSN: 0264-6021
Primary rat vascular smooth muscle cells cultured on fibronectin in the absence of serum lost smooth-muscle-specific myosin heavy chain but did not enter the cell cycle and proliferate until they were stimulated by serum. Under these conditions accumulation of Id mRNA occurred only in response to serum and was maximal during the G1 phase of the cycle.
KEMP PR, GRAINGER DJ, SHANAHAN CM, et al., 1991, THE ID GENE IS ACTIVATED BY SERUM BUT IS NOT REQUIRED FOR DEDIFFERENTIATION IN RAT VASCULAR SMOOTH-MUSCLE CELLS, BIOCHEMICAL JOURNAL, Vol: 277, Pages: 285-288, ISSN: 0264-6021
- Author Web Link
- Cite
- Citations: 32
This data is extracted from the Web of Science and reproduced under a licence from Thomson Reuters. You may not copy or re-distribute this data in whole or in part without the written consent of the Science business of Thomson Reuters.