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@article{Connolly:2017:10.1002/2211-5463.12369,
author = {Connolly, M and Garfield, B and Crosby, A and Morrell, N and Wort, SJ and Kemp, P},
doi = {10.1002/2211-5463.12369},
journal = {FEBS Open Bio},
pages = {339--348},
title = {miR-322-5p targets IGF-1 and is suppressed in the heart of rats with pulmonary hypertension},
url = {http://dx.doi.org/10.1002/2211-5463.12369},
volume = {8},
year = {2017}
}

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TY  - JOUR
AB  - Pulmonary arterial hypertension (PAH) is characterised by remodelling of the pulmonary vasculature leading to right ventricular hypertrophy. Here we show that miR-322-5p (the rodent orthologue of miR-424-5p) expression is decreased in the right ventricle of monocrotaline-treated rats, a model of PAH, whereas a putative target insulin-like growth factor 1 (IGF-1) is increased. IGF-1 mRNA was enriched 16-fold in RNA immunoprecipitated with Ago2, indicating binding to miR-322-5p. In cell transfection experiments, miR-322-5p suppressed the activity of a luciferase reporter containing a section of the IGF-1 3′ untranslated region (UTR) as well as IGF-1 mRNA and protein levels. Taken together, these data suggest that miR-322 targets IGF-1, a process downregulated in PAH-related RV hypertrophy.
AU  - Connolly,M
AU  - Garfield,B
AU  - Crosby,A
AU  - Morrell,N
AU  - Wort,SJ
AU  - Kemp,P
DO  - 10.1002/2211-5463.12369
EP  - 348
PY  - 2017///
SN  - 2211-5463
SP  - 339
TI  - miR-322-5p targets IGF-1 and is suppressed in the heart of rats with pulmonary hypertension
T2  - FEBS Open Bio
UR  - http://dx.doi.org/10.1002/2211-5463.12369
UR  - http://hdl.handle.net/10044/1/55081
VL  - 8
ER  -

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