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@article{Rog-Zielinska:2018:europace/euy245,
author = {Rog-Zielinska, EA and Kong, CHT and Zgierski-Johnston, CM and Verkade, P and Mantell, J and Cannell, MB and Kohl, P},
doi = {europace/euy245},
journal = {Europace},
pages = {120--124},
title = {Species differences in themorphology of transverse tubule openings in cardiomyocytes},
url = {http://dx.doi.org/10.1093/europace/euy245},
volume = {20},
year = {2018}
}

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TY  - JOUR
AB  - AimsThe ultrastructure of ventricular cardiomyocyte T-tubule connections with the outer cell surface (‘mouth’ regions) has been reported to differ between mice and rabbits. In mice, T-tubule mouths form convoluted narrow spaces filled with electron-dense matter that impedes diffusion between T-tubular lumen and bulk extracellular space. Here, we explore whether T-tubule mouths are also constricted in rat (another murine model used frequently for cardiac research) and whether pig and human T-tubule mouth configurations are structurally more similar to mice or rabbits.Methods and resultsWe used chemically-fixed tissue and high-pressure frozen isolated cardiomyocytes to compare T-tubule mouth architecture using transmission electron microscopy and three-dimensional electron tomography. We find that rat T-tubular mouth architecture is more similar to that of rabbits than mice, lacking the marked tortuosity and electron-dense ground substance that obstructs access to deeper portions of the T-tubular system in mice. Pilot observations in larger mammals (pig, human) suggest that mouse may be the least representative animal model of T-tubule connectivity with the outer cell surface in larger mammals.ConclusionRat T-tubular system architecture appears to be more similar in size and topology to larger mammals than mice. T-tubular mouth topology may contribute to differences in experimental model behaviour, underscoring the challenge of appropriate model selection for research into cell and tissue function.
AU  - Rog-Zielinska,EA
AU  - Kong,CHT
AU  - Zgierski-Johnston,CM
AU  - Verkade,P
AU  - Mantell,J
AU  - Cannell,MB
AU  - Kohl,P
DO  - europace/euy245
EP  - 124
PY  - 2018///
SN  - 1099-5129
SP  - 120
TI  - Species differences in themorphology of transverse tubule openings in cardiomyocytes
T2  - Europace
UR  - http://dx.doi.org/10.1093/europace/euy245
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000454046700014&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://academic.oup.com/europace/article/20/suppl_3/iii120/5202173
UR  - http://hdl.handle.net/10044/1/84700
VL  - 20
ER  -

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