Imperial College London
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ProfessorPeterKohl

Faculty of Medicine, National Heart & Lung Institute

Visiting Professor
 
 
 
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Contact

 

p.kohl Website

 
 
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Location

 

Heart Science CentreHarefield Hospital

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Summary

 

Publications

Citation

BibTex format

@article{Burton:2017:10.1016/j.bpj.2017.07.026,
author = {Burton, RAB and Rog-Zielinska, EA and Corbett, AD and Peyronnet, R and Bodi, I and Fink, M and Sheldon, J and Hoenger, A and Calaghan, SC and Bub, G and Kohl, P},
doi = {10.1016/j.bpj.2017.07.026},
journal = {Biophysical Journal},
pages = {1047--1059},
title = {Caveolae in rabbit ventricular myocytes: distribution and dynamic diminution after cell isolation},
url = {http://dx.doi.org/10.1016/j.bpj.2017.07.026},
volume = {113},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Caveolae are signal transduction centers, yet their subcellular distribution and preservation in cardiac myocytes after cell isolation are not well documented. Here, we quantify caveolae located within 100 nm of the outer cell surface membrane in rabbit single-ventricular cardiomyocytes over 8 h post-isolation and relate this to the presence of caveolae in intact tissue. Hearts from New Zealand white rabbits were either chemically fixed by coronary perfusion or enzymatically digested to isolate ventricular myocytes, which were subsequently fixed at 0, 3, and 8 h post-isolation. In live cells, the patch-clamp technique was used to measure whole-cell plasma membrane capacitance, and in fixed cells, caveolae were quantified by transmission electron microscopy. Changes in cell-surface topology were assessed using scanning electron microscopy. In fixed ventricular myocardium, dual-axis electron tomography was used for three-dimensional reconstruction and analysis of caveolae in situ. The presence and distribution of surface-sarcolemmal caveolae in freshly isolated cells matches that of intact myocardium. With time, the number of surface-sarcolemmal caveolae decreases in isolated cardiomyocytes. This is associated with a gradual increase in whole-cell membrane capacitance. Concurrently, there is a significant increase in area, diameter, and circularity of sub-sarcolemmal mitochondria, indicative of swelling. In addition, electron tomography data from intact heart illustrate the regular presence of caveolae not only at the surface sarcolemma, but also on transverse-tubular membranes in ventricular myocardium. Thus, caveolae are dynamic structures, present both at surface-sarcolemmal and transverse-tubular membranes. After cell isolation, the number of surface-sarcolemmal caveolae decreases significantly within a time frame relevant for single-cell research. The concurrent increase in cell capacitance suggests that membrane incorporation of surface-sarcolemmal caveolae unde
AU  - Burton,RAB
AU  - Rog-Zielinska,EA
AU  - Corbett,AD
AU  - Peyronnet,R
AU  - Bodi,I
AU  - Fink,M
AU  - Sheldon,J
AU  - Hoenger,A
AU  - Calaghan,SC
AU  - Bub,G
AU  - Kohl,P
DO  - 10.1016/j.bpj.2017.07.026
EP  - 1059
PY  - 2017///
SN  - 0006-3495
SP  - 1047
TI  - Caveolae in rabbit ventricular myocytes: distribution and dynamic diminution after cell isolation
T2  - Biophysical Journal
UR  - http://dx.doi.org/10.1016/j.bpj.2017.07.026
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000410462300009&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.sciencedirect.com/science/article/pii/S0006349517308512?via%3Dihub
UR  - http://hdl.handle.net/10044/1/84674
VL  - 113
ER  -
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