Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Asai:2019:10.3389/fmicb.2019.02630,
author = {Asai, M and Li, Y and Singh, Khara J and Robertson, B and Langford, P and Newton, S},
doi = {10.3389/fmicb.2019.02630},
journal = {Frontiers in Microbiology},
title = {Galleria mellonella: a novel infection model for screening potential anti-mycobacterial compounds against members of the Mycobacterium tuberculosis complex},
url = {http://dx.doi.org/10.3389/fmicb.2019.02630},
volume = {10},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Drug screening models have a vital role in the development of novel antimycobacterial agents which are urgently needed to tackle drug-resistant tuberculosis (TB). We recently established the larvae of the insect Galleria mellonella (greater wax moth) as a novel infection model for the Mycobacterium tuberculosis complex. Here we demonstrate its use as a rapid and reproducible screen to evaluate antimycobacterial drug efficacy using larvae infected with bioluminescent Mycobacterium bovis BCG lux. Treatment improved larval survival outcome and, with the exception of pyrazinamide, was associated with a significant reduction in in vivo mycobacterial bioluminescence over a 96 hour period compared to the untreated controls. Isoniazid and rifampicin displayed the greatest in vivo efficacy and survival outcome. Thus G. mellonella, infected with bioluminescent mycobacteria, can rapidly determine in vivo drug efficacy, and has the potential to significantly reduce and/or replace the number of animals used in TB research.
AU  - Asai,M
AU  - Li,Y
AU  - Singh,Khara J
AU  - Robertson,B
AU  - Langford,P
AU  - Newton,S
DO  - 10.3389/fmicb.2019.02630
PY  - 2019///
SN  - 1664-302X
TI  - Galleria mellonella: a novel infection model for screening potential anti-mycobacterial compounds against members of the Mycobacterium tuberculosis complex
T2  - Frontiers in Microbiology
UR  - http://dx.doi.org/10.3389/fmicb.2019.02630
UR  - http://hdl.handle.net/10044/1/74426
VL  - 10
ER  -
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