Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
//

Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
//

Location

 

236Wright Fleming WingSt Mary's Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Crispim:2020:femspd/ftaa066,
author = {Crispim, JS and da, Silva TF and Sanches, NM and da, Silva GC and Pereira, MF and Rossi, CC and Li, Y and Terra, VS and Vohra, P and Wren, BW and Langford, PR and Bossé, JT and Bazzolli, DMS},
doi = {femspd/ftaa066},
journal = {Pathogens and Disease},
pages = {1--12},
title = {Serovar-dependent differences in Hfq-regulated phenotypes in Actinobacillus pleuropneumoniae.},
url = {http://dx.doi.org/10.1093/femspd/ftaa066},
volume = {78},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The RNA chaperone Hfq regulates diverse processes in numerous bacteria. In this study, we compared phenotypes (growth rate, adherence, response to different stress conditions, and virulence in Galleria mellonella) of wild-type (WT) and isogenic hfq mutants of three serovars (1, 8 and 15) of the porcine pathogen A. pleuropneumoniae. Similar growth in rich broth was seen for all strains except Ap1hfq, which showed slightly reduced growth throughout the 24 hour time course, and the complemented Ap8hfqC mutant had a prolonged lag phase. Differences were seen between the three serovar WT strains regarding adherence, stress response and virulence in G. mellonella, and deletion of hfq affected some, but not all of these phenotypes, depending on serovar. Complementation by expression of cloned hfq from an endogenous promoter only restored some WT phenotypes, indicating that complex regulatory networks may be involved, and that levels of Hfq may be as important as presence/absence of the protein regarding its contribution to gene regulation. Our results support that Hfq is a pleiotropic global regulator in A. pleuropneumoniae, but serovar-related differences exist. These results highlight the importance of testing multiple strains/serovars within a given species when determining contributions of global regulators, such as Hfq, to expression of complex phenotypes.
AU  - Crispim,JS
AU  - da,Silva TF
AU  - Sanches,NM
AU  - da,Silva GC
AU  - Pereira,MF
AU  - Rossi,CC
AU  - Li,Y
AU  - Terra,VS
AU  - Vohra,P
AU  - Wren,BW
AU  - Langford,PR
AU  - Bossé,JT
AU  - Bazzolli,DMS
DO  - femspd/ftaa066
EP  - 12
PY  - 2020///
SN  - 2049-632X
SP  - 1
TI  - Serovar-dependent differences in Hfq-regulated phenotypes in Actinobacillus pleuropneumoniae.
T2  - Pathogens and Disease
UR  - http://dx.doi.org/10.1093/femspd/ftaa066
UR  - https://www.ncbi.nlm.nih.gov/pubmed/33095236
UR  - https://academic.oup.com/femspd/advance-article/doi/10.1093/femspd/ftaa066/5936557
UR  - http://hdl.handle.net/10044/1/84148
VL  - 78
ER  -
Download