Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Srijuntongsiri:2022:10.1128/spectrum.01311-21,
author = {Srijuntongsiri, G and Mhoowai, A and Samngamnim, S and Assavacheep, P and Bossé, JT and Langford, PR and Posayapisit, N and Leartsakulpanich, U and Songsungthong, W},
doi = {10.1128/spectrum.01311-21},
journal = {Microbiology Spectrum},
title = {Novel DNA Markers for Identification of Actinobacillus pleuropneumoniae},
url = {http://dx.doi.org/10.1128/spectrum.01311-21},
volume = {10},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Actinobacillus pleuropneumoniae causes porcine pleuropneumonia, an important disease in the pig industry. Accurate and sensitive diagnostics such as DNA-based diagnostics are essential for preventing or responding to an outbreak. The specificity of DNA-based diagnostics depends on species-specific markers. Previously, an insertion element was found within an A. pleuropneumoniae-specific gene commonly used for A. pleuropneumoniae detection, prompting the need for additional species-specific markers. Herein, 12 marker candidates highly conserved (99 - 100% identity) among 34 A. pleuropneumoniae genomes (covering 13 serovars) were identified to be A. pleuropneumoniae-specific in silico, as these sequences are distinct from 30 genomes of 13 other Actinobacillus and problematic [Actinobacillus] species and more than 1700 genomes of other bacteria in the Pasteurellaceae family. Five marker candidates are within the apxIVA gene, a known A. pleuropneumoniae-specific gene, validating our in silico marker discovery method. Seven other A. pleuropneumoniae-specific marker candidates within the eamA, nusG, sppA, xerD, ybbN, ycfL, and ychJ genes were validated by polymerase chain reaction (PCR) to be specific to 129 isolates of A. pleuropneumoniae (covering all 19 serovars), but not to four closely related Actinobacillus species, four [Actinobacillus] species, or seven other bacterial species. This is the first study to identify A. pleuropneumoniae-specific markers through genome mining. Seven novel A. pleuropneumoniae-specific DNA markers were identified by a combination of in silico and molecular methods and can serve as additional or alternative targets for A. pleuropneumoniae diagnostics, potentially leading to better control of the disease. IMPORTANCE Species-specific markers are crucial for infectious disease diagnostics. Mutations within a marker sequence can lead to false-negative results, inappropriate treatment, and economic loss. The availability of several species-spe
AU  - Srijuntongsiri,G
AU  - Mhoowai,A
AU  - Samngamnim,S
AU  - Assavacheep,P
AU  - Bossé,JT
AU  - Langford,PR
AU  - Posayapisit,N
AU  - Leartsakulpanich,U
AU  - Songsungthong,W
DO  - 10.1128/spectrum.01311-21
PY  - 2022///
SN  - 2165-0497
TI  - Novel DNA Markers for Identification of Actinobacillus pleuropneumoniae
T2  - Microbiology Spectrum
UR  - http://dx.doi.org/10.1128/spectrum.01311-21
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34985298
UR  - http://hdl.handle.net/10044/1/93921
VL  - 10
ER  -
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