Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
//

Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
//

Location

 

236Wright Fleming WingSt Mary's Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Stringer:2022,
author = {Stringer, OW and Li, Y and Bosse, JT and Forrest, MS and Hernandez-Garcia, J and Tucker, AW and Nunes, T and Costa, F and Mortensen, P and Velazquez, E and Penny, P and Rodriguez, Manzano J and Georgiou, P and Langford, P},
journal = {Frontiers in Veterinary Science},
title = {Rapid detection of A. pleuropneumoniae from clinical samples using recombinase polymerase amplification},
url = {https://www.frontiersin.org/articles/10.3389/fvets.2022.805382/full},
volume = {9},
year = {2022}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Actinobacillus pleuropneumoniae (APP) is the causative agent of porcine pleuropneumonia, resulting in high economic impact worldwide. There are currently 19 known serovars of APP, with different ones being predominant in specific geographic regions. Outbreaks of pleuropneumonia, characterized by sudden respiratory difficulties and high mortality, can occur when infected pigs are brought into naïve herds, or by those carrying different serovars. Good biosecurity measures include regular diagnostic testing for surveillance purposes. Current gold standard diagnostic techniques lacksensitivity (bacterial culture), require expensive thermocycling machinery (PCR) and are time consuming (culture and PCR). Here we describe the development of an isothermal point-of-care diagnostic test - utilizing recombinase polymerase amplification (RPA) for the detection of APP,targeting the species-specific apxIVA gene. Our APP-RPA diagnostic test achieved a sensitivity of 10 copies/µL using a strain of APP serovar 8, which is the most prevalent serovar in the UK. Additionally, our APP-RPA assay achieved a clinical sensitivity and specificity of 84.3% and 100%, respectively,across 61 extracted clinical samples obtained from farms located in England and Portugal. Using a small subset (n = 14) of the lung tissue samples, we achieved a clinical sensitivity and specificity of 76.9% and 100%, respectively) using lung imprints made on FTA cards tested directly in the APP- RPA reaction. Our results demonstrate that our APP-RPA assay enables a suitable rapid and sensitive screening tool for this important veterinary pathogen.
AU  - Stringer,OW
AU  - Li,Y
AU  - Bosse,JT
AU  - Forrest,MS
AU  - Hernandez-Garcia,J
AU  - Tucker,AW
AU  - Nunes,T
AU  - Costa,F
AU  - Mortensen,P
AU  - Velazquez,E
AU  - Penny,P
AU  - Rodriguez,Manzano J
AU  - Georgiou,P
AU  - Langford,P
PY  - 2022///
SN  - 2297-1769
TI  - Rapid detection of A. pleuropneumoniae from clinical samples using recombinase polymerase amplification
T2  - Frontiers in Veterinary Science
UR  - https://www.frontiersin.org/articles/10.3389/fvets.2022.805382/full
UR  - http://hdl.handle.net/10044/1/95848
VL  - 9
ER  -
Download