Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Cunha:2022:10.3389/fmicb.2022.1017278,
author = {Cunha, da Silva G and Rossi, CC and Nogueira, Rosa J and Moreno, Sanches N and Lopes, Cardoso D and Li, Y and Witney, A and Gould, K and Pereira, Fontes P and Callaghan, A and Bosse, J and Langford, P and Bazzolli, D},
doi = {10.3389/fmicb.2022.1017278},
journal = {Frontiers in Microbiology},
title = {Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae},
url = {http://dx.doi.org/10.3389/fmicb.2022.1017278},
volume = {13},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The RNA chaperone Hfq promotes the association of small RNAs (sRNAs) with cognate mRNAs, controlling the expression of bacterial phenotype. Actinobacillus pleuropneumoniae hfq mutants strains are attenuated for virulence in pigs, impaired in the ability to form biofilms, and more susceptible to stress, but knowledge of the extent of sRNA involvement is limited. Here, using A. pleuropneumoniae strain MIDG2331 (serovar 8), 14 sRNAs were identified by co-immunoprecipitation with Hfq and the expression of eight, identified as trans-acting sRNAs, were confirmed by Northern blotting. We focused on one of these sRNAs, named Rna01, containing a putative promoter for RpoE (stress regulon) recognition. Knockout mutants of rna01 and a double knockout mutant of rna01 and hfq, both had decreased biofilm formation and hemolytic activity, attenuation for virulence in Galleria mellonella, altered stress susceptibility, and an altered outer membrane protein profile. Rna01 affected extracellular vesicle production, size and toxicity in G. mellonella. qRT-PCR analysis of rna01 and putative cognate mRNA targets indicated that Rna01 is associated with the extracytoplasmic stress response. This work increases our understanding of the multilayered and complex nature of the influence of Hfq-dependent sRNAs on the physiology and virulence of A. pleuropneumoniae.
AU  - Cunha,da Silva G
AU  - Rossi,CC
AU  - Nogueira,Rosa J
AU  - Moreno,Sanches N
AU  - Lopes,Cardoso D
AU  - Li,Y
AU  - Witney,A
AU  - Gould,K
AU  - Pereira,Fontes P
AU  - Callaghan,A
AU  - Bosse,J
AU  - Langford,P
AU  - Bazzolli,D
DO  - 10.3389/fmicb.2022.1017278
PY  - 2022///
SN  - 1664-302X
TI  - Identification of small RNAs associated with RNA chaperone Hfq reveals a new stress response regulator in Actinobacillus pleuropneumoniae
T2  - Frontiers in Microbiology
UR  - http://dx.doi.org/10.3389/fmicb.2022.1017278
UR  - http://hdl.handle.net/10044/1/99712
VL  - 13
ER  -
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