Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Luan:2022:10.3389/fmicb.2022.928307,
author = {Luan, T and Wang, L and Luan, H and Zhang, Y and Langford, P and Liu, S and Zhang, W and Li, G},
doi = {10.3389/fmicb.2022.928307},
journal = {Frontiers in Microbiology},
title = {A CRISPR/Cas12a-assisted rapid detection platform by biosensing the apxIVA of Actinobacillus pleuropneumoniae},
url = {http://dx.doi.org/10.3389/fmicb.2022.928307},
volume = {13},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Actinobacillus pleuropneumoniae is an important respiratory pig pathogen that causes substantial losses in the worldwide swine industry. Chronic or subclinical infection with no apparent clinical symptoms poses a challenge for preventing transmission between herds. Rapid diagnostics is important for the control of epidemic diseases. In this study, we formulated an A. pleuropneumoniae species-specific apxIVA-based CRISPR/Cas12a-assisted rapid detection platform (Card) that combines recombinase polymerase amplification (RPA) of target DNA and subsequent Cas12a ssDNase activation. Card has a detection limit of 10 CFUs of A. pleuropneumoniae, and there is no cross-reactivity with other common swine pathogens. The detection process can be completed in 1 h, and there was 100% agreement between the conventional apxIVA-based PCR and Card in detecting A. pleuropneumoniae in lung samples. Microplate fluorescence readout enables high-throughput use in diagnostic laboratories, and naked eye and lateral flow test readouts enable use at the point of care. We conclude that Card is a versatile, rapid, accurate molecular diagnostic platform suitable for use in both laboratory and low-resource settings.
AU  - Luan,T
AU  - Wang,L
AU  - Luan,H
AU  - Zhang,Y
AU  - Langford,P
AU  - Liu,S
AU  - Zhang,W
AU  - Li,G
DO  - 10.3389/fmicb.2022.928307
PY  - 2022///
SN  - 1664-302X
TI  - A CRISPR/Cas12a-assisted rapid detection platform by biosensing the apxIVA of Actinobacillus pleuropneumoniae
T2  - Frontiers in Microbiology
UR  - http://dx.doi.org/10.3389/fmicb.2022.928307
UR  - http://hdl.handle.net/10044/1/99699
VL  - 13
ER  -
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