Imperial College London
Alternate

ProfessorPaulLangford

Faculty of Medicine, Department of Infectious Disease

Professor of Paediatric Infectious Diseases
 
 
 
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Contact

 

+44 (0)20 7594 3359p.langford Website

 
 
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Location

 

236Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Nahar:2023:nar/gkad091,
author = {Nahar, N and Tram, G and Jen, FE-C and Phillips, ZN and Weinert, LA and Bosse, JT and Jabbari, JS and Gouil, Q and Du, MRM and Ritchie, ME and Bowden, R and Langford, PR and Tucker, AW and Jennings, MP and Turni, C and Blackall, PJ and Atack, JM},
doi = {nar/gkad091},
journal = {Nucleic Acids Research},
pages = {3240--3260},
title = {Actinobacillus pleuropneumoniae encodes multiple phase-variable DNA methyltransferases that control distinct phasevarions},
url = {http://dx.doi.org/10.1093/nar/gkad091},
volume = {51},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Actinobacillus pleuropneumoniae is the cause of porcine pleuropneumonia, a severe respiratory tract infection that is responsible for major economic losses to the swine industry. Many host-adapted bacterial pathogens encode systems known as phasevarions (phase-variable regulons). Phasevarions result from variable expression of cytoplasmic DNA methyltransferases. Variable expression results in genome-wide methylation differences within a bacterial population, leading to altered expression of multiple genes via epigenetic mechanisms. Our examination of a diverse population of A. pleuropneumoniae strains determined that Type I and Type III DNA methyltransferases with the hallmarks of phase variation were present in this species. We demonstrate that phase variation is occurring in these methyltransferases, and show associations between particular Type III methyltransferase alleles and serovar. Using Pacific BioSciences Single-Molecule, Real-Time (SMRT) sequencing and Oxford Nanopore sequencing, we demonstrate the presence of the first ever characterised phase-variable, cytosine-specific Type III DNA methyltransferase. Phase variation of distinct Type III DNA methyltransferase in A. pleuropneumoniae results in the regulation of distinct phasevarions, and in multiple phenotypic differences relevant to pathobiology. Our characterisation of these newly described phasevarions in A. pleuropneumoniae will aid in the selection of stably expressed antigens, and direct and inform development of a rationally designed subunit vaccine against this major veterinary pathogen.
AU  - Nahar,N
AU  - Tram,G
AU  - Jen,FE-C
AU  - Phillips,ZN
AU  - Weinert,LA
AU  - Bosse,JT
AU  - Jabbari,JS
AU  - Gouil,Q
AU  - Du,MRM
AU  - Ritchie,ME
AU  - Bowden,R
AU  - Langford,PR
AU  - Tucker,AW
AU  - Jennings,MP
AU  - Turni,C
AU  - Blackall,PJ
AU  - Atack,JM
DO  - nar/gkad091
EP  - 3260
PY  - 2023///
SN  - 0305-1048
SP  - 3240
TI  - Actinobacillus pleuropneumoniae encodes multiple phase-variable DNA methyltransferases that control distinct phasevarions
T2  - Nucleic Acids Research
UR  - http://dx.doi.org/10.1093/nar/gkad091
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000939369100001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://academic.oup.com/nar/advance-article/doi/10.1093/nar/gkad091/7058191?login=true
UR  - http://hdl.handle.net/10044/1/102911
VL  - 51
ER  -
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