Imperial College London
Alternate

DrPradeepLuther

Faculty of Medicine, National Heart & Lung Institute

Senior Research Fellow
 
 
 
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Contact

 

p.luther Website

 
 
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Location

 

ICTEM buildingHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Burgoyne:2019:10.1073/pnas.1902235116,
author = {Burgoyne, T and Heumann, J and Morris, E and Knupp, C and Liu, J and Reedy, M and Taylor, K and Wang, K and Luther, P},
doi = {10.1073/pnas.1902235116},
journal = {Proceedings of the National Academy of Sciences},
pages = {15534--15539},
title = {Three-dimensional structure of the basketweave Z-band in midshipman fish sonic muscle},
url = {http://dx.doi.org/10.1073/pnas.1902235116},
volume = {116},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Striated muscle enables movement in all animals by the contraction of myriads of sarcomeres joined end to end by the Z-bands. The contraction is due to tension generated in each sarcomere between overlapping arrays of actin and myosin filaments. At the Z-band, actin filaments from adjoining sarcomeres overlap and are cross-linked in a regular pattern mainly by the protein α-actinin. The Z-band is dynamic, reflected by the 2 regular patterns seen in transverse section electron micrographs; the so-called small-square and basketweave forms. Although these forms are attributed, respectively, to relaxed and actively contracting muscles, the basketweave form occurs in certain relaxed muscles as in the muscle studied here. We used electron tomography and subtomogram averaging to derive the 3D structure of the Z-band in the swimbladder sonic muscle of type I male plainfin midshipman fish (Porichthys notatus), into which we docked the crystallographic structures of actin and α-actinin. The α-actinin links run diagonally between connected pairs of antiparallel actin filaments and are oriented at an angle of about 25° away from the actin filament axes. The slightly curved and flattened structure of the α-actinin rod has a distinct fit into the map. The Z-band model provides a detailed understanding of the role of α-actinin in transmitting tension between actin filaments in adjoining sarcomeres.
AU  - Burgoyne,T
AU  - Heumann,J
AU  - Morris,E
AU  - Knupp,C
AU  - Liu,J
AU  - Reedy,M
AU  - Taylor,K
AU  - Wang,K
AU  - Luther,P
DO  - 10.1073/pnas.1902235116
EP  - 15539
PY  - 2019///
SN  - 0027-8424
SP  - 15534
TI  - Three-dimensional structure of the basketweave Z-band in midshipman fish sonic muscle
T2  - Proceedings of the National Academy of Sciences
UR  - http://dx.doi.org/10.1073/pnas.1902235116
UR  - https://www.pnas.org/content/early/2019/07/17/1902235116
UR  - http://hdl.handle.net/10044/1/71761
VL  - 116
ER  -
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