Imperial College London
Alternate

Professor Paul M. Matthews

Faculty of Medicine, Department of Brain Sciences

Edmond and Lily Safra Chair, Head of Department
 
 
 
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Contact

 

+44 (0)20 7594 2855p.matthews

 
 
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Assistant

 

Ms Siobhan Dillon +44 (0)20 7594 2855

 
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Location

 

E502Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Owen:2017:10.1177/0271678X17710182,
author = {Owen, DRJ and Narayan, N and Wells, L and Healy, L and Smyth, E and Rabiner, EA and Galloway, D and Williams, JB and Lehr, J and Mandhir, H and Peferoen, LAN and Taylor, PC and Amor, S and Antel, JP and Matthews, PM and Moore, CS},
doi = {10.1177/0271678X17710182},
journal = {Journal of Cerebral Blood Flow and Metabolism},
pages = {2679--2690},
title = {Pro-inflammatory activation of primary microglia and macrophages increases 18kDa Translocator Protein (TSPO) expression in rodents but not humans},
url = {http://dx.doi.org/10.1177/0271678X17710182},
volume = {37},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The 18kDa Translocator Protein (TSPO) is the most commonly used tissue-specific marker of inflammation in positron emission tomography (PET) studies. It is expressed in myeloid cells such as microglia and macrophages, and in rodent myeloid cells expression increases with cellular activation. We assessed the effect of myeloid cell activation on TSPO gene expression in both primary human and rodent microglia and macrophages in vitro, and also measured TSPO radioligand binding with 3H-PBR28 in primary human macrophages. As observed previously, we found that TSPO expression increases (∼9-fold) in rodent-derived macrophages and microglia upon pro-inflammatory stimulation. However, TSPO expression does not increase with classical pro-inflammatory activation in primary human microglia (fold change 0.85 [95% CI 0.58–1.12], p = 0.47). In contrast, pro-inflammatory activation of human monocyte-derived macrophages is associated with a reduction of both TSPO gene expression (fold change 0.60 [95% CI 0.45–0.74], p = 0.02) and TSPO binding site abundance (fold change 0.61 [95% CI 0.49–0.73], p < 0.0001). These findings have important implications for understanding the biology of TSPO in activated macrophages and microglia in humans. They are also clinically relevant for the interpretation of PET studies using TSPO targeting radioligands, as they suggest changes in TSPO expression may reflect microglial and macrophage density rather than activation phenotype.
AU  - Owen,DRJ
AU  - Narayan,N
AU  - Wells,L
AU  - Healy,L
AU  - Smyth,E
AU  - Rabiner,EA
AU  - Galloway,D
AU  - Williams,JB
AU  - Lehr,J
AU  - Mandhir,H
AU  - Peferoen,LAN
AU  - Taylor,PC
AU  - Amor,S
AU  - Antel,JP
AU  - Matthews,PM
AU  - Moore,CS
DO  - 10.1177/0271678X17710182
EP  - 2690
PY  - 2017///
SN  - 1559-7016
SP  - 2679
TI  - Pro-inflammatory activation of primary microglia and macrophages increases 18kDa Translocator Protein (TSPO) expression in rodents but not humans
T2  - Journal of Cerebral Blood Flow and Metabolism
UR  - http://dx.doi.org/10.1177/0271678X17710182
UR  - http://hdl.handle.net/10044/1/48231
VL  - 37
ER  -
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