Imperial College London
Alternate

Dr Paul F. McKay

Faculty of Medicine, Department of Infectious Disease

Advanced Research Fellow
 
 
 
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Contact

 

+44 (0)20 7594 2542p.mckay

 
 
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Location

 

125 (Shattock Group)Wright Fleming WingSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Spencer:2021:10.1101/2021.01.28.428665,
author = {Spencer, AJ and McKay, PF and Belij-Rammerstorfer, S and Ulaszewska, M and Bissett, CD and Hu, K and Samnuan, K and Blakney, AK and Wright, D and Sharpe, HR and Gilbride, C and Truby, A and Allen, ER and Gilbert, SC and Shattock, RJ and Lambe, T},
doi = {10.1101/2021.01.28.428665},
title = {Heterologous vaccination regimens with self-amplifying RNA and Adenoviral COVID vaccines induce robust immune responses in mice},
url = {http://dx.doi.org/10.1101/2021.01.28.428665},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:p>Several vaccines have demonstrated efficacy against SARS-CoV-2 mediated disease, yet there is limited data on the immune response induced by heterologous vaccination regimens using alternate vaccine modalities. Here, we present a detailed description of the immune response, in mice, following vaccination with a self-amplifying RNA (saRNA) vaccine and an adenoviral vectored vaccine (ChAdOx1 nCoV-19/AZD1222) against SARS-CoV-2. We demonstrate that antibody responses are higher in two dose heterologous vaccination regimens than single dose regimens. Neutralising titres after heterologous prime-boost were at least comparable or higher than the titres measured after homologous prime boost vaccination with viral vectors. Importantly, the cellular immune response after a heterologous regimen is dominated by cytotoxic T cells and Th1<jats:sup>+</jats:sup> CD4 T cells which is superior to the response induced in homologous vaccination regimens in mice. These results underpin the need for clinical trials to investigate the immunogenicity of heterologous regimens with alternate vaccine technologies.</jats:p>
AU  - Spencer,AJ
AU  - McKay,PF
AU  - Belij-Rammerstorfer,S
AU  - Ulaszewska,M
AU  - Bissett,CD
AU  - Hu,K
AU  - Samnuan,K
AU  - Blakney,AK
AU  - Wright,D
AU  - Sharpe,HR
AU  - Gilbride,C
AU  - Truby,A
AU  - Allen,ER
AU  - Gilbert,SC
AU  - Shattock,RJ
AU  - Lambe,T
DO  - 10.1101/2021.01.28.428665
PY  - 2021///
TI  - Heterologous vaccination regimens with self-amplifying RNA and Adenoviral COVID vaccines induce robust immune responses in mice
UR  - http://dx.doi.org/10.1101/2021.01.28.428665
ER  -
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