Imperial College London
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ProfessorPhilipMolyneaux

Faculty of Medicine, National Heart & Lung Institute

Professor of Interstitial Lung Disease
 
 
 
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p.molyneaux

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Asghar:2023:10.1186/s12931-023-02333-5,
author = {Asghar, S and Monkley, S and Smith, DJF and Hewitt, RJ and Grime, K and Murray, LA and Overed-Sayer, CL and Molyneaux, PL},
doi = {10.1186/s12931-023-02333-5},
journal = {Respiratory Research},
pages = {1--16},
title = {Epithelial senescence in idiopathic pulmonary fibrosis is propagated by small extracellular vesicles},
url = {http://dx.doi.org/10.1186/s12931-023-02333-5},
volume = {24},
year = {2023}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease that affects 3 million people worldwide. Senescence and small extracellular vesicles (sEVs) have been implicated in the pathogenesis of IPF, although how sEVs promote disease remains unclear. Here, we profile sEVs from bronchial epithelial cells and determine small RNA (smRNA) content. METHODS: Conditioned media was collected and sEVs were isolated from normal human bronchial epithelial cells (NHBEs) and IPF-diseased human bronchial epithelial cells (DHBEs). RESULTS: Increased sEV release from DHBEs compared to NHBEs (n = 4; p < 0.05) was detected by nanoparticle tracking analysis. NHBEs co-cultured with DHBE-derived sEVs for 72 h expressed higher levels of SA-β-Gal and γH2AX protein, p16 and p21 RNA and increased secretion of IL6 and IL8 proteins (all n = 6-8; p < 0.05). sEVs were also co-cultured with healthy air-liquid interface (ALI) cultures and similar results were observed, with increases in p21 and p16 gene expression and IL6 and IL8 (basal and apical) secretion (n = 6; p < 0.05). Transepithelial electrical resistance (TEER) measurements, a reflection of epithelial barrier integrity, were decreased upon the addition of DHBE-derived sEVs (n = 6; p < 0.05). smRNA-sequencing identified nineteen significantly differentially expressed miRNA in DHBE-derived sEVs compared to NHBE-derived sEVs, with candidate miRNAs validated by qPCR (all n = 5; p < 0.05). Four of these miRNAs were upregulated in NHBEs co-cultured with DHBE-derived sEVs and three in healthy ALI cultures co-cultured with DHBE-derived sEVs (n = 3-4; p < 0.05). CONCLUSIONS: This data demonstrates that DHBE-derived sEVs transfer senescence to neighbouring healthy cells, promoting the disease state in IPF.
AU  - Asghar,S
AU  - Monkley,S
AU  - Smith,DJF
AU  - Hewitt,RJ
AU  - Grime,K
AU  - Murray,LA
AU  - Overed-Sayer,CL
AU  - Molyneaux,PL
DO  - 10.1186/s12931-023-02333-5
EP  - 16
PY  - 2023///
SN  - 1465-9921
SP  - 1
TI  - Epithelial senescence in idiopathic pulmonary fibrosis is propagated by small extracellular vesicles
T2  - Respiratory Research
UR  - http://dx.doi.org/10.1186/s12931-023-02333-5
UR  - https://www.ncbi.nlm.nih.gov/pubmed/36788603
UR  - https://respiratory-research.biomedcentral.com/articles/10.1186/s12931-023-02333-5
UR  - http://hdl.handle.net/10044/1/102805
VL  - 24
ER  -
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