Imperial College London
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ProfessorPaoloMuraro

Faculty of Medicine, Department of Brain Sciences

Professor of Neurology - Neuroimmunology and Immunotherapy
 
 
 
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Contact

 

p.muraro Website

 
 
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Assistant

 

Mrs Gearoidin Beazley +44 (0)20 7594 7047

 
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Location

 

E415Burlington DanesHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Amoriello:2021:10.1016/j.ebiom.2021.103429,
author = {Amoriello, R and Chernigovskaya, M and Greiff, V and Carnasciali, A and Massacesi, L and Barilaro, A and Repice, AM and Biagioli, T and Aldinucci, A and Muraro, PA and Laplaud, DA and Lossius, A and Ballerini, C},
doi = {10.1016/j.ebiom.2021.103429},
journal = {EBioMedicine},
pages = {103429--103429},
title = {TCR repertoire diversity in Multiple Sclerosis: High-dimensional bioinformatics analysis of sequences from brain, cerebrospinal fluid and peripheral blood},
url = {http://dx.doi.org/10.1016/j.ebiom.2021.103429},
volume = {68},
year = {2021}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BACKGROUND: T cells play a key role in the pathogenesis of multiple sclerosis (MS), a chronic, inflammatory, demyelinating disease of the central nervous system (CNS). Although several studies recently investigated the T-cell receptor (TCR) repertoire in cerebrospinal fluid (CSF) of MS patients by high-throughput sequencing (HTS), a deep analysis on repertoire similarities and differences among compartments is still missing. METHODS: We performed comprehensive bioinformatics on high-dimensional TCR Vβ sequencing data from published and unpublished MS and healthy donors (HD) studies. We evaluated repertoire polarization, clone distribution, shared CDR3 amino acid sequences (CDR3s-a.a.) across repertoires, clone overlap with public databases, and TCR similarity architecture. FINDINGS: CSF repertoires showed a significantly higher public clones percentage and sequence similarity compared to peripheral blood (PB). On the other hand, we failed to reject the null hypothesis that the repertoire polarization is the same between CSF and PB. One Primary-Progressive MS (PPMS) CSF repertoire differed from the others in terms of TCR similarity architecture. Cluster analysis splits MS from HD. INTERPRETATION: In MS patients, the presence of a physiological barrier, the blood-brain barrier, does not impact clone prevalence and distribution, but impacts public clones, indicating CSF as a more private site. We reported a high Vβ sequence similarity in the CSF-TCR architecture in one PPMS. If confirmed it may be an interesting insight into MS progressive inflammatory mechanisms. The clustering of MS repertoires from HD suggests that disease shapes the TCR Vβ clonal profile. FUNDING: This study was partly financially supported by the Italian Multiple Sclerosis Foundation (FISM), that contributed to Ballerini-DB data collection (grant #2015 R02).
AU  - Amoriello,R
AU  - Chernigovskaya,M
AU  - Greiff,V
AU  - Carnasciali,A
AU  - Massacesi,L
AU  - Barilaro,A
AU  - Repice,AM
AU  - Biagioli,T
AU  - Aldinucci,A
AU  - Muraro,PA
AU  - Laplaud,DA
AU  - Lossius,A
AU  - Ballerini,C
DO  - 10.1016/j.ebiom.2021.103429
EP  - 103429
PY  - 2021///
SN  - 2352-3964
SP  - 103429
TI  - TCR repertoire diversity in Multiple Sclerosis: High-dimensional bioinformatics analysis of sequences from brain, cerebrospinal fluid and peripheral blood
T2  - EBioMedicine
UR  - http://dx.doi.org/10.1016/j.ebiom.2021.103429
UR  - https://www.ncbi.nlm.nih.gov/pubmed/34127432
UR  - https://www.sciencedirect.com/science/article/pii/S235239642100222X?via%3Dihub
UR  - http://hdl.handle.net/10044/1/90749
VL  - 68
ER  -
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