Imperial College London
Alternate

ProfessorPeterNixon

Faculty of Natural Sciences, Department of Life Sciences

Professor of Biochemistry
 
 
 
//

Contact

 

+44 (0)20 7594 5269p.nixon

 
 
//

Location

 

705Sir Ernst Chain BuildingSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Selão:2020:10.3389/fpls.2019.01700,
author = {Selão, TT and Jebarani, J and Ismail, NA and Norling, B and Nixon, PJ},
doi = {10.3389/fpls.2019.01700},
journal = {Frontiers in Plant Science},
title = {Enhanced production of D-lactate in cyanobacteria by re-routing photosynthetic cyclic and pseudo-cyclic electron flow},
url = {http://dx.doi.org/10.3389/fpls.2019.01700},
volume = {10},
year = {2020}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Cyanobacteria are promising chassis strains for the photosynthetic production of platform and specialty chemicals from carbon dioxide. Their efficient light harvesting and metabolic flexibility has allowed a wide range of biomolecules, such as the bioplastic polylactate precursor D lactate, to be produced, though usually at relatively low yields. In order to increase photosynthetic electron flow towards the production of D-lactate, we have generated several strains of the marine cyanobacterium Synechococcus sp. PCC 7002 (Syn7002) with deletions in genes involved in cyclic or pseudo-cyclic electron flow around photosystem I. Using a variant of the Chlamydomonas reinhardtii D-lactate dehydrogenase (LDHSRT 25 , engineered to efficiently utilize NADPH in vivo), we show that deletion of either of the two flavodiiron flv homologues (involved in pseudo-cyclic electron transport) or the Syn7002 pgr5 homologue (proposed to be a vital part of the cyclic electron transport pathway) is able to increase D-lactate production in Syn7002 strains expressing LDHSRT 29 and the Escherichia coli LldP (lactate permease), especially at low temperature (25 °C) and 0.04% (v/v) CO2, though at elevated temperatures (38 °C) and/or high (1%) CO2 concentrations the effect was less obvious. The Δpgr5 background seemed to be particularly beneficial at 25 °C and 0.04% (v/v) CO2, with a nearly 7-fold increase in D lactate accumulation in comparison to the wild-type background (≈1000 vs ≈150 mg/L) and decreased side effects in comparison to the flv deletion strains. Overall, our results show that manipulation of photosynthetic electron flow is a viable strategy to increase production of platform chemicals in cyanobacteria under ambient conditions.
AU  - Selão,TT
AU  - Jebarani,J
AU  - Ismail,NA
AU  - Norling,B
AU  - Nixon,PJ
DO  - 10.3389/fpls.2019.01700
PY  - 2020///
SN  - 1664-462X
TI  - Enhanced production of D-lactate in cyanobacteria by re-routing photosynthetic cyclic and pseudo-cyclic electron flow
T2  - Frontiers in Plant Science
UR  - http://dx.doi.org/10.3389/fpls.2019.01700
UR  - http://hdl.handle.net/10044/1/75478
VL  - 10
ER  -
Download