Imperial College London
Alternate

ProfessorPeterNixon

Faculty of Natural Sciences, Department of Life Sciences

Professor of Biochemistry
 
 
 
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Contact

 

+44 (0)20 7594 5269p.nixon

 
 
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Location

 

705Sir Ernst Chain BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Knoppova:2016:pcp/pcw115,
author = {Knoppova, J and Yu, J and Konik, P and Nixon, PJ and Komenda, J},
doi = {pcp/pcw115},
journal = {Plant and Cell Physiology},
pages = {1921--1931},
title = {CyanoP Is involved in the early steps of Photosystem two assembly in thecyanobacterium synechocystis sp. PCC 6803},
url = {http://dx.doi.org/10.1093/pcp/pcw115},
volume = {57},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Although the Photosystem II (PSII) complex is highly conserved in cyanobacteria and chloroplasts, the PsbU and PsbV subunits stabilizing the oxygen-evolving Mn4 CaO5 cluster in cyanobacteria are absent in chloroplasts and have been replaced by the PsbP and PsbQ subunits. There is, however, a distant cyanobacterial homologue of PsbP, termed CyanoP, of unknown function. Here we show that CyanoP plays a role in the early stages of PSII biogenesis in Synechocystis sp. PCC 6803. CyanoP is present in the PSII reaction centre assembly complex (RCII) lacking both the CP47 and CP43 modules and binds to the smaller D2 module. A small amount of larger PSII core complexes co-purifying with FLAG-tagged CyanoP indicates that CyanoP can accompany PSII on most of its assembly pathway. A role in biogenesis is supported by the accumulation of unassembled D1 precursor and impaired formation of RCII in a mutant lacking CyanoP. Interestingly, the pull-down preparations of CyanoP-FLAG from a strain lacking CP47 also contained PsbO indicating engagement of this protein with PSII at a much earlier stage in assembly than previously assumed.
AU  - Knoppova,J
AU  - Yu,J
AU  - Konik,P
AU  - Nixon,PJ
AU  - Komenda,J
DO  - pcp/pcw115
EP  - 1931
PY  - 2016///
SN  - 1471-9053
SP  - 1921
TI  - CyanoP Is involved in the early steps of Photosystem two assembly in thecyanobacterium synechocystis sp. PCC 6803
T2  - Plant and Cell Physiology
UR  - http://dx.doi.org/10.1093/pcp/pcw115
UR  - http://hdl.handle.net/10044/1/33600
VL  - 57
ER  -
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