Imperial College London
Alternate

ProfessorPeterO'Hare

Faculty of Medicine, Department of Infectious Disease

Chair in Virology
 
 
 
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Contact

 

+44 (0)20 7594 9517p.ohare Website

 
 
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Location

 

Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Abaitua:2013,
author = {Abaitua, F and Zia, R and Hollinshead, M and O'Hare, P},
journal = {Journal of Virology},
title = {Polarised cell migration during cell-to-cell transmission of herpes simplex virus in human skin keratinocytes.},
year = {2013}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - In addition to transmission involving extracellular free particles, a generally accepted model of virus propagation is one wherein virus replicates in one cell producing infectious particles that transmit to the next cell via cell junctions or induced polarised contacts. This mechanism of spread is especially important in the presence of neutralising antibody and the concept underpins analysis of virus spread, plaque size, viral and host functions and general mechanisms of virus propagation.  Here we demonstrate a novel process involved in cell-to-cell transmission of herpes simplex virus (HSV) in human skin cells that has not previously been appreciated. Using time lapse microscopy of fluorescent viruses we show that HSV infection induces the polarised migration of skin cells into the site of infection. In the presence of neutralising antibody, uninfected skin cells migrate to the initial site of infection and spread over infected cells, to become infected in a spatially confined cluster containing hundreds of cells. The cells in this cluster do not undergo cytocidal cell lysis but harbour abundant enveloped particles within cells and cell-free virus within interstitial regions below the cluster surface. Cells at the base and outside the cluster were generally negative for virus immediate-early expression.  We further show using spatially separated monolayer assays, that at least one component of this induced migration is the paracrine stimulation of a cytotactic response from infected cells to uninfected cells. The existence of this process changes our concept of virus transmission and the potential functions, virus and host factors involved.
AU  - Abaitua,F
AU  - Zia,R
AU  - Hollinshead,M
AU  - O'Hare,P
PY  - 2013///
TI  - Polarised cell migration during cell-to-cell transmission of herpes simplex virus in human skin keratinocytes.
T2  - Journal of Virology
ER  -
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