Publications
69 results found
Bui M, Ojala PM, Whittaker G, 1996, Cells and viruses: An infectious relationship, Pages: 199-201, ISSN: 0962-8924
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- Citations: 2
OJALA PM, BAMFORD DH, 1995, IN-VITRO TRANSCRIPTION OF THE DOUBLE-STRANDED-RNA BACTERIOPHAGE-PHI-6 IS INFLUENCED BY PURINE NTPS AND CALCIUM, VIROLOGY, Vol: 207, Pages: 400-408, ISSN: 0042-6822
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- Citations: 23
Bamford DH, Ojala PM, Frilander M, et al., 1995, Isolation, purification, and function of assembly intermediates and subviral particles of bacteriophages PRD1 and σ6, Methods in Molecular Genetics, Vol: 6, Pages: 455-474, ISSN: 1067-2389
This chapter describes the techniques used in laboratory for obtaining large quantities of relatively pure viral and subviral particles for biochemical and structural studies. The model systems in use are two bacterial viruses, PRD1 and Ф6, which both have a lipid membrane as their structural component. Thus, the particles in both cases include nucleic acid, protein, and lipid constituents. All protein concentration measurements are carried out by the Coomassie blue method of Bradford using bovine serum albumin (BSA) as a standard. The sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDSPAGE) system is a modified Laemmli system and is described in the chapter. To separate the PRD1 and the Ф6 structural proteins, a 16% acrylamide concentration is used. For the preparation of reproducible sucrose gradients, a tilted tube rotation device is used, where the sucrose gradient is formed from the light and heavy components by the rotation of the tube. When the centrifugation procedures do not indicate the rotor, a microcentrifuge (Eppendorf or equivalent) is referred. © 1995 Elsevier Inc. All rights reserved.
OJALA PM, PAATERO AO, BAMFORD DH, 1994, NTP BINDING INDUCES CONFORMATIONAL-CHANGES IN THE DOUBLE-STRANDED-RNA BACTERIOPHAGE-O6 SUBVIRAL PARTICLES, VIROLOGY, Vol: 205, Pages: 170-178, ISSN: 0042-6822
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- Citations: 10
OJALA PM, JUUTI JT, BAMFORD DH, 1993, PROTEIN-P4 OF DOUBLE-STRANDED-RNA BACTERIOPHAGE OMICRON-6 IS ACCESSIBLE ON THE NUCLEOCAPSID SURFACE - EPITOPE MAPPING AND ORIENTATION OF THE PROTEIN, JOURNAL OF VIROLOGY, Vol: 67, Pages: 2879-2886, ISSN: 0022-538X
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- Citations: 31
KENNEY JM, HANTULA J, FULLER SD, et al., 1992, BACTERIOPHAGE-PHI-6 ENVELOPE ELUCIDATED BY CHEMICAL CROSS-LINKING, IMMUNODETECTION, AND CRYOELECTRON MICROSCOPY, VIROLOGY, Vol: 190, Pages: 635-644, ISSN: 0042-6822
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- Citations: 39
BAMFORD JKH, HANNINEN AL, PAKULA TM, et al., 1991, GENOME ORGANIZATION OF MEMBRANE-CONTAINING BACTERIOPHAGE-PRD1, VIROLOGY, Vol: 183, Pages: 658-676, ISSN: 0042-6822
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- Citations: 73
OLKKONEN VM, OJALA PM, BAMFORD DH, 1991, GENERATION OF INFECTIOUS NUCLEOCAPSIDS BY INVITRO ASSEMBLY OF THE SHELL PROTEIN ON TO THE POLYMERASE COMPLEX OF THE DSRNA BACTERIOPHAGE PHI-6, JOURNAL OF MOLECULAR BIOLOGY, Vol: 218, Pages: 569-581, ISSN: 0022-2836
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- Citations: 51
OJALA PM, ROMANTSCHUK M, BAMFORD DH, 1990, PURIFIED PHI-6 NUCLEOCAPSIDS ARE CAPABLE OF PRODUCTIVE INFECTION OF HOST-CELLS WITH PARTIALLY DISRUPTED OUTER MEMBRANES, VIROLOGY, Vol: 178, Pages: 364-372, ISSN: 0042-6822
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- Citations: 39
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