Imperial College London

Peter Openshaw - Professor of Experimental Medicine

Faculty of MedicineNational Heart & Lung Institute

Senior Consul, Professor of Experimental Medicine
 
 
 
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Contact

 

+44 (0)20 7594 3854p.openshaw Website CV

 
 
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Assistant

 

Ms Gale Lewis +44 (0)20 7594 0944

 
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Location

 

353Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Peng:2020:10.1101/2020.06.05.134551,
author = {Peng, Y and Mentzer, AJ and Liu, G and Yao, X and Yin, Z and Dong, D and Dejnirattisai, W and Rostron, T and Supasa, P and Liu, C and Lopez-Camacho, C and Slon-Campos, J and Zhao, Y and Stuart, D and Paeson, G and Grimes, J and Antson, F and Bayfield, OW and Hawkins, DE and Ker, D-S and Turtle, L and Subramaniam, K and Thomson, P and Zhang, P and Dold, C and Ratcliff, J and Simmonds, P and de, Silva T and Sopp, P and Wellington, D and Rajapaksa, U and Chen, Y-L and Salio, M and Napolitani, G and Paes, W and Borrow, P and Kessler, B and Fry, JW and Schwabe, NF and Semple, MG and Baillie, KJ and Moore, S and Openshaw, PJ and Ansari, A and Dunachie, S and Barnes, E and Frater, J and Kerr, G and Goulder, P and Lockett, T and Levin, R and Cornall, RJ and Conlon, C and Klenerman, P and McMichael, A and Screaton, G and Mongkolsapaya, J and Knight, JC and Ogg, G and Dong, T},
doi = {10.1101/2020.06.05.134551},
journal = {bioRxiv},
title = {Broad and strong memory CD4 + and CD8 + T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients.},
url = {http://dx.doi.org/10.1101/2020.06.05.134551},
year = {2020}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - COVID-19 is an ongoing global crisis in which the development of effective vaccines and therapeutics will depend critically on understanding the natural immunity to the virus, including the role of SARS-CoV-2-specific T cells. We have conducted a study of 42 patients following recovery from COVID-19, including 28 mild and 14 severe cases, comparing their T cell responses to those of 16 control donors. We assessed the immune memory of T cell responses using IFNγ based assays with overlapping peptides spanning SARS-CoV-2 apart from ORF1. We found the breadth, magnitude and frequency of memory T cell responses from COVID-19 were significantly higher in severe compared to mild COVID-19 cases, and this effect was most marked in response to spike, membrane, and ORF3a proteins. Total and spike-specific T cell responses correlated with the anti-Spike, anti-Receptor Binding Domain (RBD) as well as anti-Nucleoprotein (NP) endpoint antibody titre (p<0.001, <0.001 and =0.002). We identified 39 separate peptides containing CD4 + and/or CD8 + epitopes, which strikingly included six immunodominant epitope clusters targeted by T cells in many donors, including 3 clusters in spike (recognised by 29%, 24%, 18% donors), two in the membrane protein (M, 32%, 47%) and one in the nucleoprotein (Np, 35%). CD8+ responses were further defined for their HLA restriction, including B4001-restricted T cells showing central memory and effector memory phenotype. In mild cases, higher frequencies of multi-cytokine producing M- and NP-specific CD8 + T cells than spike-specific CD8 + T cells were observed. They furthermore showed a higher ratio of SARS-CoV-2-specific CD8 + to CD4 + T cell responses. Immunodominant epitope clusters and peptides containing T cell epitopes identified in this study will provide critical tools to study the role of virus-specific T cells in control and resolution of SARS-CoV-2 infections. The identification of T cell specificity and functionality associated wi
AU - Peng,Y
AU - Mentzer,AJ
AU - Liu,G
AU - Yao,X
AU - Yin,Z
AU - Dong,D
AU - Dejnirattisai,W
AU - Rostron,T
AU - Supasa,P
AU - Liu,C
AU - Lopez-Camacho,C
AU - Slon-Campos,J
AU - Zhao,Y
AU - Stuart,D
AU - Paeson,G
AU - Grimes,J
AU - Antson,F
AU - Bayfield,OW
AU - Hawkins,DE
AU - Ker,D-S
AU - Turtle,L
AU - Subramaniam,K
AU - Thomson,P
AU - Zhang,P
AU - Dold,C
AU - Ratcliff,J
AU - Simmonds,P
AU - de,Silva T
AU - Sopp,P
AU - Wellington,D
AU - Rajapaksa,U
AU - Chen,Y-L
AU - Salio,M
AU - Napolitani,G
AU - Paes,W
AU - Borrow,P
AU - Kessler,B
AU - Fry,JW
AU - Schwabe,NF
AU - Semple,MG
AU - Baillie,KJ
AU - Moore,S
AU - Openshaw,PJ
AU - Ansari,A
AU - Dunachie,S
AU - Barnes,E
AU - Frater,J
AU - Kerr,G
AU - Goulder,P
AU - Lockett,T
AU - Levin,R
AU - Cornall,RJ
AU - Conlon,C
AU - Klenerman,P
AU - McMichael,A
AU - Screaton,G
AU - Mongkolsapaya,J
AU - Knight,JC
AU - Ogg,G
AU - Dong,T
DO - 10.1101/2020.06.05.134551
PY - 2020///
TI - Broad and strong memory CD4 + and CD8 + T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients.
T2 - bioRxiv
UR - http://dx.doi.org/10.1101/2020.06.05.134551
UR - https://www.ncbi.nlm.nih.gov/pubmed/32577665
ER -