Imperial College London

Peter Openshaw - Professor of Experimental Medicine

Faculty of MedicineNational Heart & Lung Institute

Senior Consul, Professor of Experimental Medicine
 
 
 
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Contact

 

+44 (0)20 7594 3854p.openshaw Website CV

 
 
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Assistant

 

Ms Gale Lewis +44 (0)20 7594 0944

 
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Location

 

353Norfolk PlaceSt Mary's Campus

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Summary

 

Publications

Citation

BibTex format

@article{Lin:2020:infdis/jiaa448,
author = {Lin, G-L and Golubchik, T and Drysdale, S and O'Connor, D and Jefferies, K and Brown, A and de, Cesare M and Bonsall, D and Ansari, MA and Aerssens, J and Bont, L and Openshaw, P and MartinĂ³n-Torres, F and Bowden, R and Pollard, AJ and RESCEU, Investigators},
doi = {infdis/jiaa448},
journal = {J Infect Dis},
pages = {S666--S671},
title = {Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants.},
url = {http://dx.doi.org/10.1093/infdis/jiaa448},
volume = {222},
year = {2020}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - Targeted metagenomics using strand-specific libraries with target enrichment is a sensitive, generalized approach to pathogen sequencing and transcriptome profiling. Using this method, we recovered 13 (76%) complete human respiratory syncytial virus (RSV) genomes from 17 clinical respiratory samples, reconstructed the phylogeny of the infecting viruses, and detected differential gene expression between 2 RSV subgroups, specifically, a lower expression of the P gene and a higher expression of the M2 gene in RSV-A than in RSV-B. This methodology can help to relate viral genetics to clinical phenotype and facilitate ongoing population-level RSV surveillance and vaccine development. Clinical Trials Registration. NCT03627572 and NCT03756766.
AU - Lin,G-L
AU - Golubchik,T
AU - Drysdale,S
AU - O'Connor,D
AU - Jefferies,K
AU - Brown,A
AU - de,Cesare M
AU - Bonsall,D
AU - Ansari,MA
AU - Aerssens,J
AU - Bont,L
AU - Openshaw,P
AU - MartinĂ³n-Torres,F
AU - Bowden,R
AU - Pollard,AJ
AU - RESCEU,Investigators
DO - infdis/jiaa448
EP - 671
PY - 2020///
SP - 666
TI - Simultaneous Viral Whole-Genome Sequencing and Differential Expression Profiling in Respiratory Syncytial Virus Infection of Infants.
T2 - J Infect Dis
UR - http://dx.doi.org/10.1093/infdis/jiaa448
UR - https://www.ncbi.nlm.nih.gov/pubmed/32702120
VL - 222
ER -