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@article{Peng:2020:10.1101/2020.06.05.134551,
author = {Peng, Y and Mentzer, AJ and Liu, G and Yao, X and Yin, Z and Dong, D and Dejnirattisai, W and Rostron, T and Supasa, P and Liu, C and Lopez-Camacho, C and Slon-Campos, J and Zhao, Y and Stuart, D and Paeson, G and Grimes, J and Antson, F and Bayfield, OW and Hawkins, DE and Ker, D-S and Turtle, L and Subramaniam, K and Thomson, P and Zhang, P and Dold, C and Ratcliff, J and Simmonds, P and de, Silva T and Sopp, P and Wellington, D and Rajapaksa, U and Chen, Y-L and Salio, M and Napolitani, G and Paes, W and Borrow, P and Kessler, B and Fry, JW and Schwabe, NF and Semple, MG and Baillie, KJ and Moore, S and Openshaw, PJ and Ansari, A and Dunachie, S and Barnes, E and Frater, J and Kerr, G and Goulder, P and Lockett, T and Levin, R and Cornall, RJ and Conlon, C and Klenerman, P and McMichael, A and Screaton, G and Mongkolsapaya, J and Knight, JC and Ogg, G and Dong, T},
doi = {10.1101/2020.06.05.134551},
journal = {bioRxiv},
title = {Broad and strong memory CD4 + and CD8 + T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients.},
url = {http://dx.doi.org/10.1101/2020.06.05.134551},
year = {2020}
}

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TY  - JOUR
AB  - COVID-19 is an ongoing global crisis in which the development of effective vaccines and therapeutics will depend critically on understanding the natural immunity to the virus, including the role of SARS-CoV-2-specific T cells. We have conducted a study of 42 patients following recovery from COVID-19, including 28 mild and 14 severe cases, comparing their T cell responses to those of 16 control donors. We assessed the immune memory of T cell responses using IFNγ based assays with overlapping peptides spanning SARS-CoV-2 apart from ORF1. We found the breadth, magnitude and frequency of memory T cell responses from COVID-19 were significantly higher in severe compared to mild COVID-19 cases, and this effect was most marked in response to spike, membrane, and ORF3a proteins. Total and spike-specific T cell responses correlated with the anti-Spike, anti-Receptor Binding Domain (RBD) as well as anti-Nucleoprotein (NP) endpoint antibody titre (p<0.001, <0.001 and =0.002). We identified 39 separate peptides containing CD4 + and/or CD8 + epitopes, which strikingly included six immunodominant epitope clusters targeted by T cells in many donors, including 3 clusters in spike (recognised by 29%, 24%, 18% donors), two in the membrane protein (M, 32%, 47%) and one in the nucleoprotein (Np, 35%). CD8+ responses were further defined for their HLA restriction, including B4001-restricted T cells showing central memory and effector memory phenotype. In mild cases, higher frequencies of multi-cytokine producing M- and NP-specific CD8 + T cells than spike-specific CD8 + T cells were observed. They furthermore showed a higher ratio of SARS-CoV-2-specific CD8 + to CD4 + T cell responses. Immunodominant epitope clusters and peptides containing T cell epitopes identified in this study will provide critical tools to study the role of virus-specific T cells in control and resolution of SARS-CoV-2 infections. The identification of T cell specificity and functionality associated wi
AU  - Peng,Y
AU  - Mentzer,AJ
AU  - Liu,G
AU  - Yao,X
AU  - Yin,Z
AU  - Dong,D
AU  - Dejnirattisai,W
AU  - Rostron,T
AU  - Supasa,P
AU  - Liu,C
AU  - Lopez-Camacho,C
AU  - Slon-Campos,J
AU  - Zhao,Y
AU  - Stuart,D
AU  - Paeson,G
AU  - Grimes,J
AU  - Antson,F
AU  - Bayfield,OW
AU  - Hawkins,DE
AU  - Ker,D-S
AU  - Turtle,L
AU  - Subramaniam,K
AU  - Thomson,P
AU  - Zhang,P
AU  - Dold,C
AU  - Ratcliff,J
AU  - Simmonds,P
AU  - de,Silva T
AU  - Sopp,P
AU  - Wellington,D
AU  - Rajapaksa,U
AU  - Chen,Y-L
AU  - Salio,M
AU  - Napolitani,G
AU  - Paes,W
AU  - Borrow,P
AU  - Kessler,B
AU  - Fry,JW
AU  - Schwabe,NF
AU  - Semple,MG
AU  - Baillie,KJ
AU  - Moore,S
AU  - Openshaw,PJ
AU  - Ansari,A
AU  - Dunachie,S
AU  - Barnes,E
AU  - Frater,J
AU  - Kerr,G
AU  - Goulder,P
AU  - Lockett,T
AU  - Levin,R
AU  - Cornall,RJ
AU  - Conlon,C
AU  - Klenerman,P
AU  - McMichael,A
AU  - Screaton,G
AU  - Mongkolsapaya,J
AU  - Knight,JC
AU  - Ogg,G
AU  - Dong,T
DO  - 10.1101/2020.06.05.134551
PY  - 2020///
TI  - Broad and strong memory CD4 + and CD8 + T cells induced by SARS-CoV-2 in UK convalescent COVID-19 patients.
T2  - bioRxiv
UR  - http://dx.doi.org/10.1101/2020.06.05.134551
UR  - https://www.ncbi.nlm.nih.gov/pubmed/32577665
ER  -

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