Imperial College London
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Dr Poh-Choo Pang

Faculty of Natural Sciences, Department of Life Sciences

Visiting Researcher
 
 
 
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Contact

 

p.pang05 Website

 
 
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Location

 

Room 3158 Prince's GateSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Clark:2012:10.1074/mcp.M111.008730,
author = {Clark, GF and Grassi, P and Pang, P and Panico, M and Lafrenz, D and Drobnis, EZ and Baldwin, MR and Morris, HR and Haslam, SM and Schedin-Weiss, S and Sun, W and Dell, A},
doi = {10.1074/mcp.M111.008730},
journal = {Molecular & Cellular Proteomics},
title = {Tumor Biomarker Glycoproteins in the Seminal Plasma of Healthy Human Males Are Endogenous Ligands for DC-SIGN},
url = {http://dx.doi.org/10.1074/mcp.M111.008730},
volume = {11},
year = {2012}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - DC-SIGN is an immune C-type lectin that is expressed on both immature and mature dendritic cells associated with peripheral and lymphoid tissues in humans. It is a pattern recognition receptor that binds to several pathogens including HIV-1, Ebola virus, Mycobacterium tuberculosis, Candida albicans, Helicobacter pylori, and Schistosoma mansoni. Evidence is now mounting that DC-SIGN also recognizes endogenous glycoproteins, and that such interactions play a major role in maintaining immune homeostasis in humans and mice. Autoantigens (neoantigens) are produced for the first time in the human testes and other organs of the male urogenital tract under androgenic stimulus during puberty. Such antigens trigger autoimmune orchitis if the immune response is not tightly regulated within this system. Endogenous ligands for DC-SIGN could play a role in modulating such responses. Human seminal plasma glycoproteins express a high level of terminal Lewisx and Lewisy carbohydrate antigens. These epitopes react specifically with the lectin domains of DC-SIGN. However, because the expression of these sequences is necessary but not sufficient for interaction with DC-SIGN, this study was undertaken to determine if any seminal plasma glycoproteins are also endogenous ligands for DC-SIGN. Glycoproteins bearing terminal Lewisx and Lewisy sequences were initially isolated by lectin affinity chromatography. Protein sequencing established that three tumor biomarker glycoproteins (clusterin, galectin-3 binding glycoprotein, prostatic acid phosphatase) and protein C inhibitor were purified by using this affinity method. The binding of DC-SIGN to these seminal plasma glycoproteins was demonstrated in both Western blot and immunoprecipitation studies. These findings have confirmed that human seminal plasma contains endogenous glycoprotein ligands for DC-SIGN that could play a role in maintaining immune homeostasis both in the male urogenital tract and the vagina after coitus.
AU  - Clark,GF
AU  - Grassi,P
AU  - Pang,P
AU  - Panico,M
AU  - Lafrenz,D
AU  - Drobnis,EZ
AU  - Baldwin,MR
AU  - Morris,HR
AU  - Haslam,SM
AU  - Schedin-Weiss,S
AU  - Sun,W
AU  - Dell,A
DO  - 10.1074/mcp.M111.008730
PY  - 2012///
TI  - Tumor Biomarker Glycoproteins in the Seminal Plasma of Healthy Human Males Are Endogenous Ligands for DC-SIGN
T2  - Molecular & Cellular Proteomics
UR  - http://dx.doi.org/10.1074/mcp.M111.008730
UR  - http://www.mcponline.org/content/11/1/M111.008730.abstract
VL  - 11
ER  -
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