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@article{Nataraj:2015:10.1371/journal.pone.0122804,
author = {Nataraj, A},
doi = {10.1371/journal.pone.0122804},
journal = {PLoS ONE},
title = {MKAN27435 Is Required for the Biosynthesis of Higher Subclasses of Lipooligosaccharides in Mycobacterium kansasii},
url = {http://dx.doi.org/10.1371/journal.pone.0122804},
volume = {10},
year = {2015}
}

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TY  - JOUR
AB  - Lipooligosaccharides are glycolipids found in the cell wall of many mycobacterial species including the opportunistic pathogen Mycobacterium kansasii. The genome of M. kansasii ATCC12478 contains a cluster with genes orthologous to Mycobacterium marinum LOS biosynthesis genes. To initiate a genetic dissection of this cluster and demonstrate its role in LOS biosynthesis in M. kansasii, we chose MKAN27435, a gene encoding a putative glycosyltransferase. Using Specialized Transduction, a phage-based gene knockout tool previously used to generate null mutants in other mycobacteria, we generated a MKAN27435 null mutant. The mutant strain was found to be defective in the biosynthesis of higher LOS subspecies, viz LOS-IV, LOS-V, LOS-VI and LOS-VII. Additionally, a range of low abundance species were detected in the mutant strain and mass spectroscopic analysis indicated that these were shunt products generated from LOS-III by the addition of up to six molecules of a pentose.
AU  - Nataraj,A
DO  - 10.1371/journal.pone.0122804
PY  - 2015///
SN  - 1932-6203
TI  - MKAN27435 Is Required for the Biosynthesis of Higher Subclasses of Lipooligosaccharides in Mycobacterium kansasii
T2  - PLoS ONE
UR  - http://dx.doi.org/10.1371/journal.pone.0122804
UR  - https://doi.org/10.1371/journal.pone.0122804
UR  - http://hdl.handle.net/10044/1/49848
VL  - 10
ER  -

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Dr Poh-Choo Pang

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