Imperial College London
Portrait Picture

Dr Poh-Choo Pang

Faculty of Natural Sciences, Department of Life Sciences

Visiting Researcher
 
 
 
//

Contact

 

p.pang05 Website

 
 
//

Location

 

Room 3158 Prince's GateSouth Kensington Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Chen:2015:10.3390/biom5042758,
author = {Chen, Q and Müller, JS and Pang, P-C and Laval, SH and Haslam, SM and Lochmüller, H and Dell, A},
doi = {10.3390/biom5042758},
journal = {Biomolecules},
pages = {2758--2781},
title = {Global N-linked Glycosylation is Not Significantly Impaired  in Myoblasts in Congenital Myasthenic Syndromes Caused  by Defective Glutamine-Fructose-6-Phosphate  Transaminase 1 (GFPT1)},
url = {http://dx.doi.org/10.3390/biom5042758},
volume = {5},
year = {2015}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Glutamine-fructose-6-phosphate transaminase 1 (GFPT1) is the first enzyme of the hexosamine biosynthetic pathway. It transfers an amino group from glutamine to fructose-6-phosphate to yield glucosamine-6-phosphate, thus providing the precursor for uridine diphosphate N-acetylglucosamine (UDP-GlcNAc) synthesis. UDP-GlcNAc is an essential substrate for all mammalian glycosylation biosynthetic pathways and N-glycan branching is especially sensitive to alterations in the concentration of this sugar nucleotide. It has been reported that GFPT1 mutations lead to a distinct sub-class of congenital myasthenic syndromes (CMS) termed “limb-girdle CMS with tubular aggregates”. CMS are hereditary neuromuscular transmission disorders in which neuromuscular junctions are impaired. To investigate whether alterations in protein glycosylation at the neuromuscular junction might be involved in this impairment, we have employed mass spectrometric strategies to study the N-glycomes of myoblasts and myotubes derived from two healthy controls, three GFPT1 patients, and four patients with other muscular diseases, namely CMS caused by mutations in DOK7, myopathy caused by mutations in MTND5, limb girdle muscular dystrophy type 2A (LGMD2A), and Pompe disease. A comparison of the relative abundances of bi-, tri-, and tetra-antennary N-glycans in each of the cell preparations revealed that all samples exhibited broadly similar levels of branching. Moreover, although some differences were observed in the relative abundances of some of the N-glycan constituents, these variations were modest and were not confined to the GFPT1 samples. Therefore, GFPT1 mutations in CMS patients do not appear to compromise global N-glycosylation in muscle cells.
AU  - Chen,Q
AU  - Müller,JS
AU  - Pang,P-C
AU  - Laval,SH
AU  - Haslam,SM
AU  - Lochmüller,H
AU  - Dell,A
DO  - 10.3390/biom5042758
EP  - 2781
PY  - 2015///
SN  - 2218-273X
SP  - 2758
TI  - Global N-linked Glycosylation is Not Significantly Impaired  in Myoblasts in Congenital Myasthenic Syndromes Caused  by Defective Glutamine-Fructose-6-Phosphate  Transaminase 1 (GFPT1)
T2  - Biomolecules
UR  - http://dx.doi.org/10.3390/biom5042758
UR  - http://www.mdpi.com/2218-273X/5/4/2758
UR  - http://hdl.handle.net/10044/1/41141
VL  - 5
ER  -
Download