Imperial College London
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Dr Periklis (Laki) Pantazis

Faculty of Engineering, Department of Bioengineering

Reader in Advanced Optical Precision Imaging
 
 
 
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Contact

 

+44 (0)20 7594 6367p.pantazis Website CV

 
 
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Location

 

3.14Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Sugiyama:2018:10.1002/smll.201703386,
author = {Sugiyama, N and Sonay, AY and Tussiwand, R and Cohen, BE and Pantazis, P},
doi = {10.1002/smll.201703386},
journal = {Small},
pages = {1--9},
title = {Effective Labeling of Primary Somatic Stem Cells with BaTiO3 Nanocrystals for Second Harmonic Generation Imaging},
url = {http://dx.doi.org/10.1002/smll.201703386},
volume = {14},
year = {2018}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - While nanoparticles are an increasingly popular choice for labeling and tracking stem cells in biomedical applications such as cell therapy, their intracellular fate and subsequent effect on stem cell differentiation remain elusive. To establish an effective stem cell labeling strategy, the intracellular nanocrystal concentration should be minimized to avoid adverse effects, without compromising the intensity and persistence of the signal necessary for longterm tracking. Here, the use of secondharmonic generating barium titanate nanocrystals is reported, whose achievable brightness allows for high contrast stem cell labeling with at least one order of magnitude lower intracellular nanocrystals than previously reported. Their longterm photostability enables to investigate quantitatively at the single cell level their cellular fate in hematopoietic stem cells (HSCs) using both multiphoton and electron microscopy. It is found that the concentration of nanocrystals in proliferative multipotent progenitors is over 2.5fold greater compared to quiescent stem cells; this difference vanishes when HSCs enter a nonquiescent, proliferative state, while their potency remains unaffected. Understanding the nanoparticle stem cell interaction allows to establish an effective and safe nanoparticle labeling strategy into somatic stem cells that can critically contribute to an understanding of their in vivo therapeutic potential.
AU  - Sugiyama,N
AU  - Sonay,AY
AU  - Tussiwand,R
AU  - Cohen,BE
AU  - Pantazis,P
DO  - 10.1002/smll.201703386
EP  - 9
PY  - 2018///
SN  - 1613-6810
SP  - 1
TI  - Effective Labeling of Primary Somatic Stem Cells with BaTiO3 Nanocrystals for Second Harmonic Generation Imaging
T2  - Small
UR  - http://dx.doi.org/10.1002/smll.201703386
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000425744600016&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://onlinelibrary.wiley.com/doi/abs/10.1002/smll.201703386
VL  - 14
ER  -
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