Imperial College London
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Dr Periklis (Laki) Pantazis

Faculty of Engineering, Department of Bioengineering

Reader in Advanced Optical Precision Imaging
 
 
 
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Contact

 

+44 (0)20 7594 6367p.pantazis Website CV

 
 
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Location

 

3.14Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Zhang:2017:10.1038/nmeth.4222,
author = {Zhang, W and Lohman, AW and Zhuravlova, Y and Lu, X and Wiens, MD and Hoi, H and Yaganoglu, S and Mohr, MA and Kitova, EN and Klassen, JS and Pantazis, P and Thompson, RJ and Campbell, RE},
doi = {10.1038/nmeth.4222},
journal = {Nature Methods},
pages = {391--394},
title = {Optogenetic control with a photocleavable protein, PhoCl},
url = {http://dx.doi.org/10.1038/nmeth.4222},
volume = {14},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - To expand the range of experiments that are accessible with optogenetics, we developed a photocleavable protein (PhoCl) that spontaneously dissociates into two fragments after violet-light-induced cleavage of a specific bond in the protein backbone. We demonstrated that PhoCl can be used to engineer light-activatable Cre recombinase, Gal4 transcription factor, and a viral protease that in turn was used to activate opening of the large-pore ion channel Pannexin-1.
AU  - Zhang,W
AU  - Lohman,AW
AU  - Zhuravlova,Y
AU  - Lu,X
AU  - Wiens,MD
AU  - Hoi,H
AU  - Yaganoglu,S
AU  - Mohr,MA
AU  - Kitova,EN
AU  - Klassen,JS
AU  - Pantazis,P
AU  - Thompson,RJ
AU  - Campbell,RE
DO  - 10.1038/nmeth.4222
EP  - 394
PY  - 2017///
SN  - 1548-7091
SP  - 391
TI  - Optogenetic control with a photocleavable protein, PhoCl
T2  - Nature Methods
UR  - http://dx.doi.org/10.1038/nmeth.4222
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000397900500018&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/nmeth.4222
VL  - 14
ER  -
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