Imperial College London
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Dr Periklis (Laki) Pantazis

Faculty of Engineering, Department of Bioengineering

Reader in Advanced Optical Precision Imaging
 
 
 
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Contact

 

+44 (0)20 7594 6367p.pantazis Website CV

 
 
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Location

 

3.14Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Dempsey:2015:10.1038/NMETH.3405,
author = {Dempsey, WP and Georgieva, L and Helbling, PM and Sonay, AY and Truong, TV and Haffner, M and Pantazis, P},
doi = {10.1038/NMETH.3405},
journal = {Nature Methods},
pages = {645--648},
title = {In vivo single-cell labeling by confined primed conversion},
url = {http://dx.doi.org/10.1038/NMETH.3405},
volume = {12},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Spatially confined green-to-red photoconversion of fluorescent proteins with high-power, pulsed laser illumination is negligible, thus precluding optical selection of single cells in vivo. We report primed conversion, in which low-power, dual-wavelength, continuous-wave illumination results in pronounced photoconversion. With a straightforward addition to a conventional confocal microscope, we show confined primed conversion in living zebrafish and reveal the complex anatomy of individual neurons packed between neighboring cells.
AU  - Dempsey,WP
AU  - Georgieva,L
AU  - Helbling,PM
AU  - Sonay,AY
AU  - Truong,TV
AU  - Haffner,M
AU  - Pantazis,P
DO  - 10.1038/NMETH.3405
EP  - 648
PY  - 2015///
SN  - 1548-7091
SP  - 645
TI  - In vivo single-cell labeling by confined primed conversion
T2  - Nature Methods
UR  - http://dx.doi.org/10.1038/NMETH.3405
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000357405700021&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://www.nature.com/articles/nmeth.3405
VL  - 12
ER  -
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