Imperial College London
Portrait Picture

Dr Periklis (Laki) Pantazis

Faculty of Engineering, Department of Bioengineering

Reader in Advanced Optical Precision Imaging
 
 
 
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Contact

 

+44 (0)20 7594 6367p.pantazis Website CV

 
 
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Location

 

3.14Royal School of MinesSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Welling:2019:10.7554/eLife.44491,
author = {Welling, M and Mohr, MA and Ponti, A and Sabater, LR and Boni, A and Kawamura, YK and Liberali, P and Peters, AHFM and Pelczar, P and Pantazis, P},
doi = {10.7554/eLife.44491},
journal = {eLife},
pages = {1--13},
title = {Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins},
url = {http://dx.doi.org/10.7554/eLife.44491},
volume = {8},
year = {2019}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Accurate lineage reconstruction of mammalian pre-implantation development isessential for inferring the earliest cell fate decisions. Lineage tracing using global fluorescencelabeling techniques is complicated by increasing cell density and rapid embryo rotation, whichhampers automatic alignment and accurate cell tracking of obtained four-dimensional imaging datasets. Here, we exploit the advantageous properties of primed convertible fluorescent proteins (prpcFPs) to simultaneously visualize the global green and the photoconverted red population in orderto minimize tracking uncertainties over prolonged time windows. Confined primed conversion ofH2B-pr-mEosFP-labeled nuclei combined with light-sheet imaging greatly facilitates segmentation,classification, and tracking of individual nuclei from the 4-cell stage up to the blastocyst. Usinggreen and red labels as fiducial markers, we computationally correct for rotational and translationaldrift, reduce overall data size, and accomplish high-fidelity lineage tracing even for increasedimaging time intervals – addressing major concerns in the field of volumetric embryo imaging.
AU  - Welling,M
AU  - Mohr,MA
AU  - Ponti,A
AU  - Sabater,LR
AU  - Boni,A
AU  - Kawamura,YK
AU  - Liberali,P
AU  - Peters,AHFM
AU  - Pelczar,P
AU  - Pantazis,P
DO  - 10.7554/eLife.44491
EP  - 13
PY  - 2019///
SN  - 2050-084X
SP  - 1
TI  - Primed Track, high-fidelity lineage tracing in mouse pre-implantation embryos using primed conversion of photoconvertible proteins
T2  - eLife
UR  - http://dx.doi.org/10.7554/eLife.44491
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000456138300001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - https://elifesciences.org/articles/44491
UR  - http://hdl.handle.net/10044/1/79277
VL  - 8
ER  -
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