Publications
296 results found
Ford KL, Anwar M, Heys R, et al., 2019, Optimisation of laboratory methods for whole transcriptomic RNA analyses in human left ventricular biopsies and blood samples of clinical relevance, PLoS ONE, Vol: 14, ISSN: 1932-6203
This study aimed to optimise techniques for whole transcriptome and small RNA analyses on clinical tissue samples from patients with cardiovascular disease. Clinical samples often represent a particular challenge to extracting RNA of sufficient quality for robust RNA sequencing analysis, and due to availability, it is rarely possible to optimise techniques on the samples themselves. Therefore, we have used equivalent samples from pigs undergoing cardiopulmonary bypass surgery to test different protocols for optimal RNA extraction, and then validated the protocols in human samples. Here we present an assessment of the quality and quantity of RNA obtained using a variety of commercially-available RNA extraction kits on both left ventricular biopsies and blood plasma. RNA extraction from these samples presents different difficulties; left ventricular biopsies are small and fibrous, while blood plasma has a low RNA content. We have validated our optimised extraction techniques on human clinical samples collected as part of the ARCADIA (Association of non-coding RNAs with Coronary Artery Disease and type 2 Diabetes) cohort study, resulting in successful whole transcriptome and small RNA sequencing of human left ventricular tissue.
Punjabi PP, 2019, The SLEFIE - slave of the selfie, PERFUSION-UK, Vol: 34, Pages: 96-97, ISSN: 0267-6591
Bidar E, Folliguet T, Kluin J, et al., 2019, Postimplant biological aortic prosthesis degeneration: challenges in transcatheter valve implants, EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY, Vol: 55, Pages: 191-200, ISSN: 1010-7940
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- Citations: 6
Moscarelli M, Terrasini N, Nunziata A, et al., 2018, A Trial of Two Anesthetic Regimes for Minimally Invasive Mitral Valve Repair, JOURNAL OF CARDIOTHORACIC AND VASCULAR ANESTHESIA, Vol: 32, Pages: 2562-2569, ISSN: 1053-0770
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- Citations: 4
Guymer SC, Narodden S, Shanmuganathan M, et al., 2018, Longitudinal Exosomal and Tissue Expression of microRNA-208a and 208b in Acute and Chronic Rat PostMyocardial Infarction Remodelling, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Guymer SC, Narodden S, Shanmuganatha M, et al., 2018, Abstract 15875: Longitudinal exosomal and tissue expression of microRNA-208a and 208b in acute and chronic rat post-myocardial infarction remodelling, Circulation Journal, Vol: 138, ISSN: 0047-1828
Punjabi PP, 2018, POTS and PANS; "Do what you want to do, when you can do, NOW, when you CAN do", PERFUSION-UK, Vol: 33, Pages: 611-611, ISSN: 0267-6591
Eckersley MJ, Sepehripour AH, Casula R, et al., 2018, Do selective serotonin reuptake inhibitors increase the risk of bleeding or mortality following coronary artery bypass graft surgery? A meta-analysis of observational studies, Perfusion, Vol: 33, Pages: 415-422, ISSN: 1477-111X
INTRODUCTION: Depressive illness has a high prevalence in patients undergoing coronary artery bypass graft surgery (CABG). The first line treatment for depression are selective serotonin reuptake inhibitors (SSRIs) which inhibit serotonin reuptake in the presynaptic neuronal membrane and uptake by platelets, inhibiting subsequent serotonin-mediated platelet activation. This presents a theoretically increased risk of bleeding and subsequent postoperative mortality. This review aims to investigate the effects of SSRIs on postoperative bleeding, defined as the need for transfusions and re-operation for bleeding, as well as 30-day mortality in patients undergoing CABG. METHOD: Four hundred and thirty-seven papers were screened with seven meeting the full inclusion criteria. RESULTS: Meta-analysis demonstrated that SSRI use increased the risk of red blood cell transfusion (odds ratio (OR) = 1.15; 95% confidence interval (CI): 1.06-1.26), but resulted in no difference in the rate of re-operation for bleeding (OR = 1.07; 95% CI: 0.66-1.74). SSRI use had no effect on the rates of platelet (OR = 0.93; 95% CI: 0.79-1.09) or fresh frozen plasma (OR = 0.96; 95% CI: 0.74-1.24) transfusion nor on the mortality rate (OR =1.03; 95 CI: 0.90-1.17). CONCLUSION: This review demonstrates that SSRIs are largely safe in cardiac surgery as no increase in mortality was observed. However, there is a significantly raised chance of red blood cell transfusion. The heterogeneous nature of the current evidence base highlights the need for further research into SSRIs and whether any effect on patient outcomes in cardiac surgery occurs.
Punjabi PP, 2018, The professional amateur and the amateurish professional, PERFUSION-UK, Vol: 33, Pages: 413-414, ISSN: 0267-6591
Mangrolia N, Punjabi P, 2018, The cessation of oral anticoagulation following left atrial appendage surgery, FUTURE CARDIOLOGY, Vol: 14, Pages: 407-415, ISSN: 1479-6678
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- Citations: 3
Lewis-McDougall FC, Ruchaya PJ, Domenjo-Vila E, et al., 2018, Aged-senescent cells contribute to impaired heart regeneration
<jats:title>Abstract</jats:title><jats:p>Aging leads to increased cellular senescence and is associated with decreased potency of tissue-specific stem/progenitor cells. Here we have done an extensive analysis of cardiac progenitor cells (CPCs) isolated from human subjects with cardiovascular disease (n=119), aged 32-86 years. In aged subjects (>74 years old) over half of CPCs are senescent (p16<jats:sup>INK4A</jats:sup>, SA-β-gal, DNA damage γH2AX, telomere length, Senescence-Associated Secretory Phenotype (SASP)), unable to replicate, differentiate, regenerate or restore cardiac function following transplantation into the infarcted heart. SASP factors secreted by senescent CPCs renders otherwise healthy CPCs to senescence. Elimination of senescent CPCs using senolytics abrogates the SASP and its debilitative effect<jats:italic>in vitro</jats:italic>. Global elimination of senescent cells in aged mice (INK-ATTAC or wildtype mice treated with D+Q senolytics)<jats:italic>in vivo</jats:italic>activates resident CPCs (0.23±0.06% vs. 0.01±0.01% vehicle; p<0.05) and increased the number of small, proliferating Ki67-, EdU-positive cardiomyocytes (0.25±0.07% vs. 0.03±0.03% vehicle; p<0.05). Therapeutic approaches that eliminate senescent cells may alleviate cardiac deterioration with aging and rejuvenate the regenerative capacity of the heart.</jats:p>
Punjabi PP, 2018, A symbiosis and a beginning, PERFUSION-UK, Vol: 33, Pages: 6-6, ISSN: 0267-6591
Punjabi PP, 2018, The imagined order, PERFUSION-UK, Vol: 33, Pages: 248-248, ISSN: 0267-6591
Bello S, Singh C, Jayakumar S, et al., 2018, Role of Cardiomyocyte Regeneration in Mechanical Unloading Induced Reverse Cardiac Remodeling, Circulation 134(Suppl_1.20205):11 Nov 2016, ISSN: 0009-7322
Punjabi PP, 2018, Serendipity and margin of safety, Perfusion, Vol: 33, Pages: 88-88, ISSN: 1477-111X
Jhala H, Chaubey S, Punjabi PP, 2018, Inspect, respect, incise: A novel non-resection repair technique for degenerative mitral regurgitation, International Journal of Surgery, ISSN: 1743-9191
Bello SO, Singh C, Punjabi P, et al., 2017, Coronary Reperfusion Ameliorates the Deleterious Effect of Mechanical Unloading on Infarct Size and Interstitial Fibrosis After Acute Myocardial Infarction, Scientific Sessions of the American-Heart-Association / Resuscitation Science Symposium, Publisher: LIPPINCOTT WILLIAMS & WILKINS, ISSN: 0009-7322
Bello S, Singh C, Punjabi P, et al., 2017, Coronary Reperfusion Ameliorates the Deleterious Effect of Mechanical Unloading on Infarct Size and Interstitial Fibrosis After Acute Myocardial Infarction, Circulation, Vol: 136
Anastasiadis K, Murkin J, Antonitsis P, et al., 2017, The application of minimally invasive extracorporeal circulation in heart surgery: Principles, definitions and potential benefits - A position paper of the Minimal Invasive Extracorporeal Technologies International Society (MiECTiS), Kardiotechnik, Vol: 26, Pages: 97-120, ISSN: 0941-2670
Μinimal invasive extracorporeal circulation (MiECC) systems have initiat-ed important efforts within science and technology to further improve the biocom-patibility of cardiopulmonary bypass com-ponents to minimize the adverse effects and improve end-organ protection. The Minimal invasive Extra-Corporeal Technolo-gies international Society (MiECTiS) was founded to create an international forum for the exchange of ideas on clinical appli-cation and research of Minimal invasive Extra-Corporeal Circulation technology. The present work is a consensus document developed to standardize the terminology and the definition of minimal invasive ex-tracorporeal circulation technology as well as to provide recommendations for the clinical practice. The goal of this manuscript is to promote the use of MiECC systems in-to clinical practice as a multidisciplinary strategy involving cardiac surgeons, anaes-thesiologists and perfusionists.
Punjabi NP, Sahdev N, Punjabi PP, 2017, Book review: Goldberger's Clinical Electrocardiography. 9(th) Edition. A Simplified Approach Goldberger Ary L. Goldberger Zachary D Shvilkin Alexei ( 2017 ) Goldberger's Clinical Electrocardiography. 9(th) Edition. A Simplified Approach . Toronto, Canada : Elsevier, Canada , 228 pages, $90.99 . ISBN: 978-0323401692., Perfusion, Vol: 32
Punjabi PP, 2017, Healthcare - a rising burden of cost to all, PERFUSION-UK, Vol: 32, Pages: 621-621, ISSN: 0267-6591
Thompson DS, Punjabi PP, 2017, An unusual presentation of ischaemic mitral regurgitation as P2 prolapse, PERFUSION-UK, Vol: 32, Pages: 706-708, ISSN: 0267-6591
Lockwood GG, Cabreros L, Banach D, et al., 2017, Continuous bilateral thoracic paravertebral blockade for analgesia after cardiac surgery: a randomised, controlled trial, PERFUSION-UK, Vol: 32, Pages: 591-597, ISSN: 0267-6591
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- Citations: 9
Moscarelli M, Athanasiou T, Speziale G, et al., 2017, The value of adding sub-valvular procedures for chronic ischemic mitral regurgitation surgery: a meta-analysis, PERFUSION-UK, Vol: 32, Pages: 436-445, ISSN: 0267-6591
Punjabi PP, 2017, Science and the "fake news" conundrum, PERFUSION-UK, Vol: 32, Pages: 429-429, ISSN: 0267-6591
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- Citations: 2
Sarvananthan S, Punjabi PP, Lewis F, et al., 2017, The distribution and characteristics of endogenous cardiac stem cells in the atria and ventricle of the adult human heart, American College of Cardiology, Publisher: OXFORD UNIV PRESS, Pages: 535-535, ISSN: 0195-668X
Athanasopoulos LV, Casula RP, Punjabi PP, et al., 2017, A technical review of subvalvular techniques for repair of ischaemic mitral regurgitation and their associated echocardiographic and survival outcomes, Interactive Cardiovascular and Thoracic Surgery, Vol: 25, Pages: 975-982, ISSN: 1569-9293
Subvalvular techniques are gaining ground as adjunct procedures for addressing ischaemic mitral regurgitation. The aim of this study was to describe the different techniques and assess their results. A systematic review of the literature was performed. The end points of interest were recurrence of mitral regurgitation, cardiac events and early and late echocardiographic measurements. After initial screening, 450 articles were identified, of which 24 provided the best available evidence on the topic. The different subvalvular techniques had similar mortality rates when compared with the standard restrictive annuloplasty. Recurrence of mitral regurgitation was of lower degree and the remodelling process was better for these techniques. Reoperation rates were also quite low. The subvalvular techniques showed superiority, addressing more successfully the leaflet tethering. However, larger randomized studies are needed to confirm these early positive results.
Barili F, Freemantle N, Folliguet T, et al., 2017, The flaws in the detail of an observational study on transcatheter aortic valve implantation versus surgical aortic valve replacement in intermediate-risks patients, EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY, Vol: 51, Pages: 1031-1035, ISSN: 1010-7940
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- Citations: 15
Punjabi PP, 2017, Healthcare budgets across continents: at crossroads - publish or perish, PERFUSION-UK, Vol: 32, Pages: 262-262, ISSN: 0267-6591
Ajmera A, Punjabi PP, 2017, Book Review: Oxford Textbook of Fundamentals of Surgery Thomas William EG Reed Malcolm WR Wyatt Michael G ( 2016 ). Oxford Textbook of Fundamentals of Surgery . Oxford, UK : Oxford University Press , 848 pp. £145 . ISBN: 9780199665549 ., Perfusion, Vol: 32
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