Imperial College London
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Mr Prakash P Punjabi

Faculty of Medicine, National Heart & Lung Institute

Professor of Practice (Cardiothoracic Surgery)
 
 
 
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Contact

 

+44 (0)20 3313 2026p.punjabi Website

 
 
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Location

 

BN2/25 B BlockHammersmith HospitalHammersmith Campus

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Summary

 

Publications

Citation

BibTex format

@article{Skeffington:2022:10.3389/fcvm.2022.911557,
author = {Skeffington, KL and Moscarelli, M and Abdul-Ghani, S and Fiorentino, F and Emanueli, C and Reeves, BC and Punjabi, PP and Angelini, GD and Suleiman, M-S},
doi = {10.3389/fcvm.2022.911557},
journal = {Frontiers in Cardiovascular Medicine},
pages = {1--14},
title = {Pathology-related changes in cardiac energy metabolites, inflammatory response and reperfusion injury following cardioplegic arrest in patients undergoing open-heart surgery},
url = {http://dx.doi.org/10.3389/fcvm.2022.911557},
volume = {9},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Introduction: Changes in cardiac metabolites in adult patients undergoing open-heart surgery using ischemic cardioplegic arrest have largely been reported for non-ventricular tissue or diseased left ventricular tissue, with few studies attempting to assess such changes in both ventricular chambers. It is also unknown whether such changes are altered in different pathologies or linked to the degree of reperfusion injury and inflammatory response. The aim of the present work was to address these issues by monitoring myocardial metabolites in both ventricles and to establish whether these changes are linked to reperfusion injury and inflammatory/stress response in patients undergoing surgery using cold blood cardioplegia for either coronary artery bypass graft (CABG, n = 25) or aortic valve replacement (AVR, n = 16).Methods: Ventricular biopsies from both left (LV) and right (RV) ventricles were collected before ischemic cardioplegic arrest and 20 min after reperfusion. The biopsies were processed for measuring selected metabolites (adenine nucleotides, purines, and amino acids) using HPLC. Blood markers of cardiac injury (Troponin I, cTnI), inflammation (IL- 6, IL-8, Il-10, and TNFα, measured using Multiplex) and oxidative stress (Myeloperoxidase, MPO) were measured pre- and up to 72 hours post-operatively.Results: The CABG group had a significantly shorter ischemic cardioplegic arrest time (38.6 ± 2.3 min) compared to AVR group (63.0 ± 4.9 min, p = 2 x 10−6). Cardiac injury (cTnI release) was similar for both CABG and AVR groups. The inflammatory markers IL-6 and Il-8 were significantly higher in CABG patients compared to AVR patients. Metabolic markers of cardiac ischemic stress were relatively and significantly more altered in the LV of CABG patients. Comparing diabetic and non-diabetic CABG patients shows that only the RV of diabetic patients sustained major ischemic stress during reperfusion and that diabetic patients had a significantly
AU  - Skeffington,KL
AU  - Moscarelli,M
AU  - Abdul-Ghani,S
AU  - Fiorentino,F
AU  - Emanueli,C
AU  - Reeves,BC
AU  - Punjabi,PP
AU  - Angelini,GD
AU  - Suleiman,M-S
DO  - 10.3389/fcvm.2022.911557
EP  - 14
PY  - 2022///
SN  - 2297-055X
SP  - 1
TI  - Pathology-related changes in cardiac energy metabolites, inflammatory response and reperfusion injury following cardioplegic arrest in patients undergoing open-heart surgery
T2  - Frontiers in Cardiovascular Medicine
UR  - http://dx.doi.org/10.3389/fcvm.2022.911557
UR  - https://www.webofscience.com/api/gateway?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000837164900001&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=a2bf6146997ec60c407a63945d4e92bb
UR  - https://www.frontiersin.org/articles/10.3389/fcvm.2022.911557/full
UR  - http://hdl.handle.net/10044/1/101068
VL  - 9
ER  -
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