Publications

BibTex format

@article{Yu:2019:10.1002/mbo3.730,
author = {Yu, S-C and Kuemmel, F and Skoufou-Papoutsaki, M-N and Spanu, PD},
doi = {10.1002/mbo3.730},
journal = {Microbiology Open},
title = {Yeast transformation efficiency is enhanced by TORC1- and eisosome-dependent signalling},
url = {http://dx.doi.org/10.1002/mbo3.730},
volume = {8},
year = {2019}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Transformation of baker's yeast (Saccharomyces cerevisiae) plays a key role in several experimental techniques, yet the molecular mechanisms underpinning transformation are still unclear. The addition of amino acids to the growth and transformation medium increases transformation efficiency. Here, we show that target of rapamycin complex 1 (TORC1) activated by amino acids enhances transformation via ubiquitinmediated endocytosis. We created mutants of the TORC1 pathway, alphaarrestins, and eisosomerelated genes. Our results demonstrate that the TORC1Npr1Art1/Rsp5 pathway regulates yeast transformation. Based on our previous study, activation of this pathway results in up to a 200fold increase in transformation efficiency, or greater. Additionally, we suggest DNA may be taken up by domains at the membrane compartment of Can1 (MCC) in the plasma membrane formed by eisosomes. Yeast studies on transformation could be used as a platform to understand the mechanism of DNA uptake in mammalian systems, which is clinically relevant to optimize gene therapy.
AU  - Yu,S-C
AU  - Kuemmel,F
AU  - Skoufou-Papoutsaki,M-N
AU  - Spanu,PD
DO  - 10.1002/mbo3.730
PY  - 2019///
SN  - 2045-8827
TI  - Yeast transformation efficiency is enhanced by TORC1- and eisosome-dependent signalling
T2  - Microbiology Open
UR  - http://dx.doi.org/10.1002/mbo3.730
UR  - http://hdl.handle.net/10044/1/63417
VL  - 8
ER  -

Download

ProfessorPietroSpanu

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)