Imperial College London
Alternate

ProfessorPietroSpanu

Faculty of Natural Sciences, Department of Life Sciences

Professor of Molecular Plant Pathology
 
 
 
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Contact

 

+44 (0)20 7594 5384p.spanu Website

 
 
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Location

 

610Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Kusch:2022:10.1101/2022.07.26.501657,
author = {Kusch, S and Singh, M and Thieron, H and Spanu, PD and Panstruga, R},
doi = {10.1101/2022.07.26.501657},
title = {Site-specific analysis reveals candidate cross-kingdom small RNAs, tRNA and rRNA fragments, and signs of fungal RNA phasing in the barley-powdery mildew interaction},
url = {http://dx.doi.org/10.1101/2022.07.26.501657},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - <jats:title>Abstract</jats:title><jats:p>The establishment of host-microbe interactions requires molecular communication between both partners, which involves the mutual transfer of noncoding small RNAs. Previous evidence suggests that this is also true for the barley powdery mildew disease, which is caused by the fungal pathogen <jats:italic>Blumeria hordei</jats:italic>. However, previous studies lacked spatial resolution regarding the accumulation of small RNAs upon host infection by <jats:italic>B. hordei</jats:italic>. Here, we analysed site-specific small RNA repertoires in the context of the barley-<jats:italic>B. hordei</jats:italic> interaction. To this end, we dissected infected leaves into separate fractions representing different sites that are key to the pathogenic process: epiphytic fungal mycelium, infected plant epidermis, isolated haustoria, a vesicle-enriched fraction from infected epidermis, and extracellular vesicles. Unexpectedly, we discovered enrichment of specific 31- to 33-base long 5’-terminal fragments of barley 5.8S ribosomal RNA (rRNA) in extracellular vesicles and infected epidermis, as well as particular <jats:italic>B. hordei</jats:italic> tRNA fragments in haustoria. We describe canonical small RNAs from both the plant host and the fungal pathogen that may confer cross-kingdom RNA interference activity. Interestingly, we found first evidence of phased small RNAs (phasiRNAs) in <jats:italic>B. hordei</jats:italic>, a feature usually attributed to plants, which may be associated with the post-transcriptional control of fungal coding genes, pseudogenes, and transposable elements. Our data suggests a key and possibly site-specific role for cross-kingdom RNA interference and noncoding RNA fragments in the host-pathogen communication between <jats:italic>B. hordei</jats:italic> and its host barley.</jats:p>
AU  - Kusch,S
AU  - Singh,M
AU  - Thieron,H
AU  - Spanu,PD
AU  - Panstruga,R
DO  - 10.1101/2022.07.26.501657
PY  - 2022///
TI  - Site-specific analysis reveals candidate cross-kingdom small RNAs, tRNA and rRNA fragments, and signs of fungal RNA phasing in the barley-powdery mildew interaction
UR  - http://dx.doi.org/10.1101/2022.07.26.501657
ER  -
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