Imperial College London
Alternate

ProfessorPietroSpanu

Faculty of Natural Sciences, Department of Life Sciences

Professor of Molecular Plant Pathology
 
 
 
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Contact

 

+44 (0)20 7594 5384p.spanu Website

 
 
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Location

 

610Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Albaser:2016:10.1021/acs.jafc.5b05381,
author = {Albaser, A and Kazana, E and Bennett, MH and Cebeci, F and Luang-In, V and Spanu, PD and Rossiter, JT},
doi = {10.1021/acs.jafc.5b05381},
journal = {Journal of Agricultural and Food Chemistry},
pages = {1520--1527},
title = {Discovery of a Bacterial Glycoside Hydrolase Family 3 (GH3) β-Glucosidase with Myrosinase Activity from a Citrobacter Strain Isolated from Soil},
url = {http://dx.doi.org/10.1021/acs.jafc.5b05381},
volume = {64},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - A Citrobacter strain (WYE1) was isolated from a UK soil by enrichment using the glucosinolate sinigrin as sole carbon source. The enzyme myrosinase was purified using a combination of ion exchange and gel filtration to give a pure protein of approximately 66 kDa. The N-terminal amino acid and internal peptide sequence of the purified protein were determined and used to identify the gene, which, based on InterPro sequence analysis, belongs to the family GH3, contains a signal peptide, and is a periplasmic protein with a predicted molecular mass of 71.8 kDa. A preliminary characterization was carried out using protein extracts from cell-free preparations. The apparent KM and Vmax were 0.46 mM and 4.91 mmol dm–3 min–1 mg–1, respectively, with sinigrin as substrate. The optimum temperature and pH for enzyme activity were 25 °C and 6.0, respectively. The enzyme was marginally activated with ascorbate by a factor of 1.67.
AU  - Albaser,A
AU  - Kazana,E
AU  - Bennett,MH
AU  - Cebeci,F
AU  - Luang-In,V
AU  - Spanu,PD
AU  - Rossiter,JT
DO  - 10.1021/acs.jafc.5b05381
EP  - 1527
PY  - 2016///
SN  - 1520-5118
SP  - 1520
TI  - Discovery of a Bacterial Glycoside Hydrolase Family 3 (GH3) β-Glucosidase with Myrosinase Activity from a Citrobacter Strain Isolated from Soil
T2  - Journal of Agricultural and Food Chemistry
UR  - http://dx.doi.org/10.1021/acs.jafc.5b05381
UR  - http://hdl.handle.net/10044/1/41290
VL  - 64
ER  -
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