Publications

BibTex format

@article{Spanu:2016:10.1038/srep35738,
author = {Spanu, PD and Yu, SC and Dawson, A and Henderson, AC and Lockyer, EJ and Read, E and Sritharan, G and Ryan, M and Sgroi, S and Ngou, PM and Woodruff, R and Zhang, R and Teen, TCR and Liu, Y and Xiang, Y},
doi = {10.1038/srep35738},
journal = {Scientific Reports},
title = {Nutrient supplements boost yeast transformation efficiency},
url = {http://dx.doi.org/10.1038/srep35738},
volume = {6},
year = {2016}
}

Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Efficiency of yeast transformation is determined by the rate of yeast endocytosis. The aim of this study was to investigate the effect of introducing amino acids and other nutrients (inositol, adenine, or p-aminobenzoic acid) in the transformation medium to develop a highly efficient yeast transformation protocol. The target of rapamycin complex 1 (TORC1) kinase signalling complex influences the rate of yeast endocytosis. TORC signaling is induced by amino acids in the media. Here, we found that increasing the concentration of amino acids and other nutrients in the growth media lead to an increase yeast transformation efficiency up to 107 CFU per μg plasmid DNA and per 108 cells with a 13.8 kb plasmid DNA. This is over 130 times that of current published methods. This improvement may facilitate more efficient experimentation in which transformation efficiency is critical, such as yeast two-hybrid screening.
AU  - Spanu,PD
AU  - Yu,SC
AU  - Dawson,A
AU  - Henderson,AC
AU  - Lockyer,EJ
AU  - Read,E
AU  - Sritharan,G
AU  - Ryan,M
AU  - Sgroi,S
AU  - Ngou,PM
AU  - Woodruff,R
AU  - Zhang,R
AU  - Teen,TCR
AU  - Liu,Y
AU  - Xiang,Y
DO  - 10.1038/srep35738
PY  - 2016///
SN  - 2045-2322
TI  - Nutrient supplements boost yeast transformation efficiency
T2  - Scientific Reports
UR  - http://dx.doi.org/10.1038/srep35738
UR  - http://hdl.handle.net/10044/1/41090
VL  - 6
ER  -

Download

ProfessorPietroSpanu

Contact

Location

Summary

Citation

BibTex format

RIS format (EndNote, RefMan)