Imperial College London
Alternate

ProfessorPietroSpanu

Faculty of Natural Sciences, Department of Life Sciences

Professor of Molecular Plant Pathology
 
 
 
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Contact

 

+44 (0)20 7594 5384p.spanu Website

 
 
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Location

 

610Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Laur:2017:10.1111/nph.14851,
author = {Laur, J and Ramakrishnan, GB and Labbé, C and Lefebvre, F and Spanu, PD and Bélanger, RR},
doi = {10.1111/nph.14851},
journal = {New Phytologist},
pages = {713--725},
title = {Effectors involved in fungal-fungal interaction lead to a rare phenomenon of hyperbiotrophy in the tritrophic system biocontrol agent-powdery mildew-plant.},
url = {http://dx.doi.org/10.1111/nph.14851},
volume = {217},
year = {2017}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - Tritrophic interactions involving a biocontrol agent, a pathogen and a plant have been analyzed predominantly from the perspective of the biocontrol agent. We have conducted the first comprehensive transcriptomic analysis of all three organisms in an effort to understand the elusive properties of Pseudozyma flocculosa in the context of its biocontrol activity against Blumeria graminis f.sp. hordei as it parasitizes Hordeum vulgare. After inoculation of P. flocculosa, the tripartite interaction was monitored over time and samples collected for scanning electron microscopy and RNA sequencing. Based on our observations, P. flocculosa indirectly parasitizes barley, albeit transiently, by diverting nutrients extracted by B. graminis from barley leaves through a process involving unique effectors. This brings novel evidence that such molecules can also influence fungal-fungal interactions. Their release is synchronized with a higher expression of powdery mildew haustorial effectors, a sharp decline in the photosynthetic machinery of barley and a developmental peak in P. flocculosa. The interaction culminates with a collapse of B. graminis haustoria, thereby stopping P. flocculosa growth, as barley plants show higher metabolic activity. To conclude, our study has uncovered a complex and intricate phenomenon, described here as hyperbiotrophy, only achievable through the conjugated action of the three protagonists.
AU  - Laur,J
AU  - Ramakrishnan,GB
AU  - Labbé,C
AU  - Lefebvre,F
AU  - Spanu,PD
AU  - Bélanger,RR
DO  - 10.1111/nph.14851
EP  - 725
PY  - 2017///
SN  - 0028-646X
SP  - 713
TI  - Effectors involved in fungal-fungal interaction lead to a rare phenomenon of hyperbiotrophy in the tritrophic system biocontrol agent-powdery mildew-plant.
T2  - New Phytologist
UR  - http://dx.doi.org/10.1111/nph.14851
UR  - http://hdl.handle.net/10044/1/56107
VL  - 217
ER  -
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