Imperial College London
Portrait Picture

DrPanteleimonTakis

Faculty of Medicine, Department of Metabolism, Digestion and Reproduction

Honorary Research Associate
 
 
 
//

Contact

 

p.takis Website

 
 
//

Location

 

Institute of Reproductive and Developmental BiologyHammersmith Campus

//

Summary

 

Publications

Citation

BibTex format

@article{Harvey:2023:10.1021/acs.analchem.2c04066,
author = {Harvey, N and Takis, PG and Lindon, JC and Li, JV and Jiménez, B},
doi = {10.1021/acs.analchem.2c04066},
journal = {Analytical Chemistry},
pages = {3147--3152},
title = {Optimization of diffusion-ordered NMR spectroscopy experiments for high-throughput automation in human metabolic phenotyping},
url = {http://dx.doi.org/10.1021/acs.analchem.2c04066},
volume = {95},
year = {2023}
}
Download

RIS format (EndNote, RefMan)

TY  - JOUR
AB  - The diffusion-ordered nuclear magnetic resonance spectroscopy (DOSY) experiment allows the calculation of diffusion coefficient values of metabolites in complex mixtures. However, this experiment has not yet been broadly used for metabolic profiling due to lack of a standardized protocol. Here we propose a pipeline for the DOSY experimental setup and data processing in metabolic phenotyping studies. Due to the complexity of biological samples, three experiments (a standard DOSY, a relaxation-edited DOSY, and a diffusion-edited DOSY) have been optimized to provide DOSY metabolic profiles with peak-picked diffusion coefficients for over 90% of signals visible in the one-dimensional 1H general biofluid profile in as little as 3 min 36 s. The developed parameter sets and tools are straightforward to implement and can facilitate the use of DOSY for metabolic profiling of human blood plasma and urine samples.
AU  - Harvey,N
AU  - Takis,PG
AU  - Lindon,JC
AU  - Li,JV
AU  - Jiménez,B
DO  - 10.1021/acs.analchem.2c04066
EP  - 3152
PY  - 2023///
SN  - 0003-2700
SP  - 3147
TI  - Optimization of diffusion-ordered NMR spectroscopy experiments for high-throughput automation in human metabolic phenotyping
T2  - Analytical Chemistry
UR  - http://dx.doi.org/10.1021/acs.analchem.2c04066
UR  - https://pubs.acs.org/doi/10.1021/acs.analchem.2c04066
UR  - http://hdl.handle.net/10044/1/102002
VL  - 95
ER  -
Download