Publications
40 results found
Shalhoub J, Sikkel MB, Davies KJ, et al., 2014, Systems Biology of Human Atherosclerosis, VASCULAR AND ENDOVASCULAR SURGERY, Vol: 48, Pages: 5-17, ISSN: 1538-5744
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- Citations: 24
Anwar MA, Vorkas P, Li J, et al., 2014, Differential Metabolic Phenotype of Human Varicose Veins Tissue and Their Utility in Understanding Disease Pathogenesis and Identifying Potential Prognostic Biomarkers., Pages: 113-113, ISSN: 2213-333X
Markou A, Sourvinou I, Vorkas PA, et al., 2013, Clinical evaluation of microRNA expression profiling in non small cell lung cancer, LUNG CANCER, Vol: 81, Pages: 388-396, ISSN: 0169-5002
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- Citations: 169
Anwar MA, Vorkas P, Li J, et al., 2013, Prolonged Mechanical Stretch Alters the Metabolic Profile in Rat Inferior Vena Cava., Pages: 105-106, ISSN: 2213-333X
Anwar MA, Shalhoub J, Vorkas PA, et al., 2012, <i>In-vitro</i> Identification of Distinctive Metabolic Signatures of Intact Varicose Vein Tissue via Magic Angle Spinning Nuclear Magnetic Resonance Spectroscopy, EUROPEAN JOURNAL OF VASCULAR AND ENDOVASCULAR SURGERY, Vol: 44, Pages: 442-450, ISSN: 1078-5884
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- Citations: 14
Mirnezami R, Kinross JM, Vorkas PA, et al., 2012, Implementation of Molecular Phenotyping Approaches in the Personalized Surgical Patient Journey, ANNALS OF SURGERY, Vol: 255, Pages: 881-889, ISSN: 0003-4932
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- Citations: 29
Schiza C, Farkona S, Chimonidou M, et al., 2012, Detection of <i>PIK3CA</i> somatic mutations in cell-free DNA of breast cancer patients by high-resolution melting curve analysis, Publisher: AMER ASSOC CANCER RESEARCH, ISSN: 0008-5472
Dimitrakopoulos L, Vorkas PA, Georgoulias V, et al., 2012, A closed-tube methylation-sensitive high resolution melting assay (MS-HRMA) for the semi-quantitative determination of CST6 promoter methylation in clinical samples, BMC Cancer, Vol: 12, Pages: 486-486, ISSN: 1471-2407
BackgroundCST6 promoter is highly methylated in cancer, and its detection can provide important prognostic information in breast cancer patients. The aim of our study was to develop a Methylation-Sensitive High Resolution Melting Analysis (MS-HRMA) assay for the investigation of CST6 promoter methylation.MethodsWe designed primers that amplify both methylated and unmethylated CST6 sequences after sodium bisulfate (SB) treatment and used spiked control samples of fully methylated to unmethylated SB converted genomic DNA to optimize the assay. We first evaluated the assay by analyzing 36 samples (pilot training group) and further analyzed 80 FFPES from operable breast cancer patients (independent group). MS-HRMA assay results for all 116 samples were compared with Methylation-Specific PCR (MSP) and the results were comparable.ResultsThe developed assay is highly specific and sensitive since it can detect the presence of 1% methylated CST6 sequence and provides additionally a semi-quantitative estimation of CST6 promoter methylation. CST6 promoter was methylated in 39/80 (48.75%) of FFPEs with methylation levels being very different among samples. MS-HRMA and MSP gave comparable results when all samples were analyzed by both assays.ConclusionsThe developed MS-HRMA assay for CST6 promoter methylation is closed tube, highly sensitive, cost-effective, rapid and easy-to-perform. It gives comparable results to MSP in less time, while it offers the advantage of additionally providing an estimation of the level of methylation.
Vorkas PA, Poumpouridou N, Agelaki S, et al., 2010, PIK3CA Hotspot Mutation Scanning by a Novel and Highly Sensitive High-Resolution Small Amplicon Melting Analysis Method, JOURNAL OF MOLECULAR DIAGNOSTICS, Vol: 12, Pages: 697-704, ISSN: 1525-1578
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- Citations: 38
Vorkas PA, Christopoulos K, Kroupis C, et al., 2010, Mutation scanning of exon 20 of the BRCA1 gene by high-resolution melting curve analysis, Clinical Biochemistry, Vol: 43, Pages: 178-185, ISSN: 0009-9120
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