Imperial College London
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DrPanagiotisVorkas

Faculty of Medicine, Department of Surgery & Cancer

Research Associate
 
 
 
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p.vorkas

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Vorkas:2015:10.1016/j.ijcard.2015.06.048,
author = {Vorkas, PA and Isaac, G and Holmgren, A and Want, EJ and Shockcor, JP and Holmes, E and Henein, MY},
doi = {10.1016/j.ijcard.2015.06.048},
journal = {International Journal of Cardiology},
pages = {192--199},
title = {Perturbations in fatty acid metabolism and apoptosis are manifested in calcific coronary artery disease: An exploratory lipidomics study},
url = {http://dx.doi.org/10.1016/j.ijcard.2015.06.048},
volume = {197},
year = {2015}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundControversy exists concerning the beneficial or harmful effects of the presence of ectopic calcification in the coronary arteries. Additionally, further elucidation of the exact pathophysiological mechanism is needed. In this study, we sought to identify metabolic markers of vascular calcification that could assist in understanding the disease, monitoring its progress and generating hypotheses describing its pathophysiology.MethodsUntargeted lipid profiling and complementary modeling strategies were employed to compare serum samples from patients with different levels of calcific coronary artery disease (CCAD) based on their calcium score (CS). Subsequently, patients were divided into three groups: no calcification (NC; CS = 0; n = 26), mild calcification (MC; CS:1–250; n = 27) and severe (SC; CS > 250; n = 17).ResultsPhosphatidylcholine levels were found to be significantly altered in the disease states (p = 0.001–0.04). Specifically, 18-carbon fatty acyl chain (FAC) phosphatidylcholines were detected in lower levels in the SC group, while 20:4 FAC lipid species were detected in higher concentrations. A statistical trend was observed with phosphatidylcholine lipids in the MC group, showing the same tendency as with the SC group. We also observed several sphingomyelin signals present at lower intensities in SC when compared with NC or MC groups (p = 0.000001–0.01).ConclusionsThis is the first lipid profiling study reported in CCAD. Our data demonstrate dysregulations of phosphatidylcholine lipid species, which suggest perturbations in fatty acid elongation/desaturation. The altered levels of the 18-carbon and 20:4 FAC lipids may be indicative of disturbed inflammation homeostasis. The marked sphingomyelin dysregulation in SC is consistent with profound apoptosis as a potential mechanism of CCAD.
AU  - Vorkas,PA
AU  - Isaac,G
AU  - Holmgren,A
AU  - Want,EJ
AU  - Shockcor,JP
AU  - Holmes,E
AU  - Henein,MY
DO  - 10.1016/j.ijcard.2015.06.048
EP  - 199
PY  - 2015///
SN  - 1874-1754
SP  - 192
TI  - Perturbations in fatty acid metabolism and apoptosis are manifested in calcific coronary artery disease: An exploratory lipidomics study
T2  - International Journal of Cardiology
UR  - http://dx.doi.org/10.1016/j.ijcard.2015.06.048
VL  - 197
ER  -
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