Imperial College London
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DrPanagiotisVorkas

Faculty of Medicine, Department of Surgery & Cancer

Research Associate
 
 
 
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p.vorkas

 
 
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Location

 

Sir Alexander Fleming BuildingSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Djekic:2016:10.1016/j.ijcard.2016.07.214,
author = {Djekic, D and Pinto, R and Vorkas, PA and Henein, MY},
doi = {10.1016/j.ijcard.2016.07.214},
journal = {International Journal of Cardiology},
pages = {1042--1048},
title = {Replication of LC-MS untargeted lipidomics results in patients with calcific coronary disease: an interlaboratory reproducibility study},
url = {http://dx.doi.org/10.1016/j.ijcard.2016.07.214},
volume = {222},
year = {2016}
}
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RIS format (EndNote, RefMan)

TY  - JOUR
AB  - BackgroundRecently a lipidomics approach was able to identify perturbed fatty acyl chain (FAC) and sphingolipid moieties that could stratify patients according to the severity of coronary calcification, a form of subclinical atherosclerosis. Nevertheless, these findings have not yet been reproduced before generalising their application. The aim of this study was to evaluate the reproducibility of lipidomics approaches by replicating previous lipidomic findings in groups of patients with calcific coronary artery disease (CCAD).MethodsPatients were separated into the following groups based on their calcium score (CS); no calcification (CS: 0; n = 26), mild calcification (CS: 1–250; n = 27) and severe calcification (CS: > 250; n = 17). Two serum samples were collected from each patient and used for comparative analyses by 2 different laboratories, in different countries and time points using liquid chromatography coupled to mass spectrometry untargeted lipidomics methods.ResultsSix identical metabolites differentiated patients with severe coronary artery calcification from those with no calcification were found by both laboratories independently. Additionally, relative intensities from the two analyses demonstrated high correlation coefficients. Phosphatidylcholine moieties with 18-carbon FAC were identified in lower intensities and 20:4 FAC in higher intensities in the serum of diseased group. Moreover, 3 common sphingomyelins were detected.ConclusionThis is the first interlaboratory reproducibility study utilising lipidomics applications in general and specifically in patients with CCAD. Lipid profiling applications in patients with CCAD are very reproducible in highly specialised and experienced laboratories and could be applied in clinical practice in order to spare patients diagnostic radiation.
AU  - Djekic,D
AU  - Pinto,R
AU  - Vorkas,PA
AU  - Henein,MY
DO  - 10.1016/j.ijcard.2016.07.214
EP  - 1048
PY  - 2016///
SN  - 1874-1754
SP  - 1042
TI  - Replication of LC-MS untargeted lipidomics results in patients with calcific coronary disease: an interlaboratory reproducibility study
T2  - International Journal of Cardiology
UR  - http://dx.doi.org/10.1016/j.ijcard.2016.07.214
UR  - http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=WOS:000384698300219&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=1ba7043ffcc86c417c072aa74d649202
UR  - http://hdl.handle.net/10044/1/43401
VL  - 222
ER  -
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