Imperial College London
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Professor Pantelis Georgiou

Faculty of Engineering, Department of Electrical and Electronic Engineering

Professor of Biomedical Electronics
 
 
 
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Contact

 

+44 (0)20 7594 6326pantelis Website

 
 
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Location

 

902Electrical EngineeringSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Pennisi:2022:10.1101/2022.02.21.22271125,
author = {Pennisi, I and Moniri, A and Miscourides, N and Miglietta, L and Moser, N and Habgood-Coote, D and Herberg, J and Levin, M and Kaforou, M and Rodriguez-Manzano, J and Georgiou, P},
doi = {10.1101/2022.02.21.22271125},
publisher = {MedRxiv},
title = {Discrimination of bacterial and viral infection using host-RNA signatures integrated in a lab-on-a-chip technology},
url = {http://dx.doi.org/10.1101/2022.02.21.22271125},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <h4>ABSTRACT</h4> The unmet clinical need for accurate point-of-care (POC) diagnostic tests able to discriminate bacterial from viral infection demands a solution that can be used both within healthcare settings and in the field and that can also stem the tide of antimicrobial resistance. Our approach to solve this problem is to combine the use of Host-gene signatures with our Lab-on-a-chip (LoC) technology enabling low-cost LoC expression analysis to detect Infectious Disease.Host-gene expression signatures have been extensively study as a potential tool to be implemented in the diagnosis of infectious disease. On the other hand LoC technologies using Ion-sensitive field-effect transistor (ISFET) arrays, in conjunction with isothermal chemistries, are offering a promising alternative to conventional lab-based nucleic acid amplification instruments, owing to their portable and affordable nature. Currently, the data analysis of ISFET arrays are restricted to established methods by averaging the output of every sensor to give a single time-series. This simple approach makes unrealistic assumptions, leading to insufficient performance for applications that require accurate quantification such as RNA host transcriptomics. In order to reliably quantify host-gene expression on our LoC platform enabling the classification of bacterial and viral infection on chip, we propose a novel data-driven algorithm for extracting time-to-positive values from ISFET arrays. The algorithm proposed is based on modelling sensor drift with adaptive signal processing and clustering sensors based on their behaviour with unsupervised learning methods. Results show that the approach correctly outputs a time-to-positive for all the reactions, with a high correlation to RT-qLAMP (0.85, R2 = 0.98, p < 0.01), resulting in a classification accuracy of 100 % (CI, 95 - 100). By leveraging more advanced data processing methods for ISFET arrays, this work aims to bridge the gap between tr
AU  - Pennisi,I
AU  - Moniri,A
AU  - Miscourides,N
AU  - Miglietta,L
AU  - Moser,N
AU  - Habgood-Coote,D
AU  - Herberg,J
AU  - Levin,M
AU  - Kaforou,M
AU  - Rodriguez-Manzano,J
AU  - Georgiou,P
DO  - 10.1101/2022.02.21.22271125
PB  - MedRxiv
PY  - 2022///
TI  - Discrimination of bacterial and viral infection using host-RNA signatures integrated in a lab-on-a-chip technology
UR  - http://dx.doi.org/10.1101/2022.02.21.22271125
UR  - https://www.medrxiv.org/content/10.1101/2022.02.21.22271125v1
UR  - http://hdl.handle.net/10044/1/98686
ER  -
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