Imperial College London
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Professor Pantelis Georgiou

Faculty of Engineering, Department of Electrical and Electronic Engineering

Professor of Biomedical Electronics
 
 
 
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Contact

 

+44 (0)20 7594 6326pantelis Website

 
 
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Location

 

902Electrical EngineeringSouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@unpublished{Broomfield:2022:10.1101/2022.08.04.502773,
author = {Broomfield, J and Kalofonou, M and Pataillot-Meakin, T and Powell, SM and Moser, N and Bevan, CL and Georgiou, P},
doi = {10.1101/2022.08.04.502773},
publisher = {Cold Spring Harbor Laboratory},
title = {Detection of YAP1 and AR-V7 mRNA for Prostate Cancer prognosis using an ISFET Lab-On-Chip platform},
url = {http://dx.doi.org/10.1101/2022.08.04.502773},
year = {2022}
}
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RIS format (EndNote, RefMan)

TY  - UNPB
AB  - <jats:title>Abstract</jats:title><jats:p>Prostate cancer (PCa) is the second most common cause of male cancer-related death worldwide. The gold standard of treatment for advanced PCa is androgen deprivation therapy (ADT). However, eventual failure of ADT is common and leads to lethal metastatic castration resistant PCa (mCRPC). As such, the detection of relevant biomarkers in the blood for drug resistance in mCRPC patients could lead to personalized treatment options. mRNA detection is often limited by the low specificity of qPCR assays which are restricted to specialised laboratories. Here, we present a novel reversetranscription loop-mediated isothermal amplification (RT-LAMP) assay and have demonstrated its capability for sensitive detection of AR-V7 and YAP1 RNA (3×10<jats:sup>1</jats:sup> RNA copies per reaction). This work presents a foundation for the detection of circulating mRNA in PCa on a non-invasive Lab-on-chip (LoC) device for use at point-of-care. This technique was implemented onto a Lab-on-Chip platform integrating an array of chemical sensors (ion-sensitive field-effect transistors - ISFETs) for real-time detection of RNA. Detection of RNA presence was achieved through the translation of chemical signals into electrical readouts. Validation of this technique was conducted with rapid detection (<jats:italic><</jats:italic>15 min) of extracted RNA from prostate cancer cell lines 22Rv1s and DU145s.</jats:p>
AU  - Broomfield,J
AU  - Kalofonou,M
AU  - Pataillot-Meakin,T
AU  - Powell,SM
AU  - Moser,N
AU  - Bevan,CL
AU  - Georgiou,P
DO  - 10.1101/2022.08.04.502773
PB  - Cold Spring Harbor Laboratory
PY  - 2022///
TI  - Detection of YAP1 and AR-V7 mRNA for Prostate Cancer prognosis using an ISFET Lab-On-Chip platform
UR  - http://dx.doi.org/10.1101/2022.08.04.502773
UR  - https://www.biorxiv.org/content/10.1101/2022.08.04.502773v1
UR  - http://hdl.handle.net/10044/1/99107
ER  -
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