Publications
39 results found
Chaudhry A, Anand PK, Singh GS, et al., 2006, Ectopic pairing of the intercalary heterochromatin in the organophosphate pesticide treated mosquito chromosomes (Culcidae: Diptera), Cytologia, Vol: 71, Pages: 431-437, ISSN: 0011-4545
The extensive use of chemical pesticides has greatly increased the mutational load on the genome of living organisms. The problems of genetic toxicology have generated more concern than any other problem because the residual inclusion of pesticides in the environment leads to a number of direct and indirect effects on the genetic material. Induced chromosomal mutations provide a reliable index of the mutagenic potential of a chemical or a physical agent. Experience has shown that the mutagenic effect of the semilethal doses of chemicals induce a of variety of structural changes in the polytene chromosomes out of which ectopic pairings are the most frequent types of aberrations. As a consequence of these points of genetic interest, the present paper deals with the incidence of ectopic pairing of the intercalary heterochromatic bands in the polytene chromosomes of those larvae of Anopheles subpictus, which were treated with LC20 of 4 organophosphate pesticides viz. chlorpyrifos, monocrotophos, acephate and dimethoate. When compared with the data of non-treated controls the treated larvae had an elevated incidence of intercalary heterchromatic linkages in the X-chromosome and the right and left arms of autosomes 2 and 3 (2R, 2L, 3R, 3L). The results are discussed in relevance to the fact that ectopic associations are established between those heterochromatic bands which are homologous in their chemical and genetic properties. These properties are attributed to the presence of identical A : T rich nucleotide sequences resulting from gene duplications which are induced by the cellular environments altered by the pesticides. © 2006 The Japan Mendel Society.
Kaul D, Anand PK, Khanna A, 2006, Functional genomics of PPAR-γ in human immunomodulatory cells, MOLECULAR AND CELLULAR BIOCHEMISTRY, Vol: 290, Pages: 211-215, ISSN: 0300-8177
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- Citations: 10
Anand PK, Kaul D, Sharma M, 2006, Green tea polyphenol inhibits <i>Mycobacterium tuberculosis</i> survival within human macrophages, INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY, Vol: 38, Pages: 600-609, ISSN: 1357-2725
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- Citations: 57
Chaudhry A, Anand PK, 2005, Evaluation of the mutagenic potential of chlorpyrifos (CPF) using polytene chromosomes of Anopheles mosquito., J Environ Biol, Vol: 26, Pages: 145-150, ISSN: 0254-8704
Diverse cytogenetic tests are employed for short term screening of suspect environmental mutagens by using insects and mammals as models. In the present paper the polytene chromosomes of a mosquito Anopheles maculatus were used to evaluate the mutagenic potential of a widely used organophosphate pesticide chlorpyrifos-[o, o-diethyl-o-(3, 5, 6-trichloro-2-pyridyl) phosphothioate]. The results are based on the frequency of various structural aberrations encountered in the polytene chromosomes of the larvae treated with LC20 of chlorpyrifos (CPF). These aberrations were dominated by inversions, stickiness of the chromosomes, heterochromatinization of the bands and lack of polyteny. The frequency of various aberrations was highest in the left arm of chromosome number 2L followed by 2R, 3L, 3R, and X-chromosomes i.e. 2.10 +/- 0.44, 1.84 +/- 0.44, 1.57 +/- 0.54, 1.31 +/- 0.50, and 0.22 +/- 0.27 respectively. The susceptibility of different chromosomal arms to this pesticide was 2L > 2R > 3L > 3R > X and the regions prone to these aberrations have been marked on the polytene chromosome map of Anopheles maculatus.
Chaudhry A, Anand PK, 2004, Assessment of dominant lethal effects of chlorpyrifos (CPF) using mosquito genetics, Pollution Research, Vol: 23, Pages: 767-771, ISSN: 0257-8050
The screening of pesticides to assess their genotoxic potential using suitable experimental models and protocols is an important research activity in the area of genetic toxicology. The submammalian models like insects are most ideal for testing the dominant lethal effects of suspected environmental mutagens. The present paper deals with the results of dominant lethal tests (DLT) of a pesticide chlorpyrifos (CPF) on Culex quinquefaciatus. Males originating from larvae treated with LC20 were crossed with normal females and the number of hatched and unhatched eggs was statistically analyzed. The results show CPF induced dominant lethality which led to the production of unhatched eggs. It was also observed that this pesticide is harmful to the genome for the mosquito as it produced structural and numerical aberrations in the chromosomes of male meiosis and structural alterations in the larval polytene chromosomes. Copyright © Enviromedia.
Anand PK, Kaul D, 2003, Vitamin D3-dependent pathway regulates TACO gene transcription., Biochem Biophys Res Commun, Vol: 310, Pages: 876-877, ISSN: 0006-291X
Recently, gene coding for tryptophan-aspartate containing coat protein (TACO) has been recognized to play a crucial role in the survival of Mycobacterium tuberculosis within human macrophages. Since regulation of TACO gene is still poorly understood, the present study was addressed to explore the role of vitamins (A, C, D, and E) in TACO gene transcription. Such a study revealed that synergistic action of vitamin D(3) and retinoic acid (RA) had inherent ability to down-regulate TACO gene transcription in human macrophages. Based upon these results, we propose that synergistic action of RA+vitamin D may be of importance in the prevention/control of M. tuberculosis infection.
Kaul D, Anand PK, 2003, Receptor-C<sub>k</sub> regulates HMGCoA reductase gene in HL-60 cells, CURRENT SCIENCE, Vol: 85, Pages: 643-645, ISSN: 0011-3891
Kaul D, Anand PK, 2003, Regulation of <i>PPAR</i>-γ gene in human promyelocytic HL-60 cell line, LEUKEMIA RESEARCH, Vol: 27, Pages: 683-686, ISSN: 0145-2126
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- Citations: 12
Anand PK, Kaul D, 2002, Oxysterol receptor LXRα regulates <i>SREBP</i> gene expression in HL-60 cells exposed to differentiating agents, CURRENT SCIENCE, Vol: 82, Pages: 136-137, ISSN: 0011-3891
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- Citations: 1
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