Imperial College London

ProfessorPaulFrench

Faculty of Natural SciencesDepartment of Physics

Professor of Physics and Vice Dean (Research) - FoNS
 
 
 
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Contact

 

+44 (0)20 7594 7706paul.french Website

 
 
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Assistant

 

Ms Judith Baylis +44 (0)20 7594 7713

 
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Location

 

609Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

481 results found

Sonnefraud Y, Sinclair HG, Sivan Y, Foreman MR, Dunsby CW, Neil MAA, French PM, Maier SAet al., 2014, Experimental Proof of Concept of Nanoparticle-Assisted STED, NANO LETTERS, Vol: 14, Pages: 4449-4453, ISSN: 1530-6984

Journal article

Watson TJ, Andrews N, Harry E, Bugeon L, Dallman MJ, French PMW, McGinty Jet al., 2014, Remote focal scanning and sub-volume optical projection tomography

© OSA 2016. We present a platform for sub-volume optical projection tomography utilising an electrically tunable lens and tracking technology. Applied to 3D fluorescent bead phantoms and zebrafish embryos, we demonstrate an improvement in resolution and light collection efficiency with respect to conventional optical projection tomography.

Conference paper

Kumar S, Lockwood N, Ramel MC, Correia T, Ellis M, Alexandrov Y, Andrews N, Patel R, Bugeon L, Dallman MJ, Brandner S, Arridge S, Katan M, McGinty J, Frankel P, French PMWet al., 2014, In vivo multiplexed OPT and FLIM OPT of an adult zebrafish cancer disease model

© OSA 2016. We report angular multiplexed OPT and FLIM OPT applied to in vivo imaging of cancer and FRET biosensors in adult zebrafish. Multiple-spectral 3-D datasets of entire adult zebrafish can be acquired in 3 minutes.

Conference paper

Marcu L, French PMW, Elson DS, 2014, Preface, Publisher: CRC Press

The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM).

Other

Marcu L, French P, Elson DS, 2014, Chapter 1: Introduction, Fluorescence Lifetime Spectroscopy and Imaging Principles and Applications in Biomedical Diagnostics, Publisher: CRC Press, ISBN: 9781439861677

The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM).

Book chapter

Dyer B, Lagarto J, Sikkel M, French P, Dunsby C, Peters N, Lyon Aet al., 2014, THE APPLICATION OF AUTOFLUORESCENCE LIFETIME METROLOGY AS A NOVEL LABEL-FREE TECHNIQUE FOR THE ASSESSMENT OF CARDIAC DISEASE, HEART, Vol: 100, Pages: A104-A104, ISSN: 1355-6037

Journal article

Gore DM, Margineanu A, French P, O'Brart D, Dunsby C, Allan BDet al., 2014, Two-Photon Fluorescence Microscopy of Corneal Riboflavin Absorption, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, Vol: 55, Pages: 2476-2481, ISSN: 0146-0404

Journal article

Gore DM, Margineanu A, French P, O'Brart D, Dunsby C, Allan BDSet al., 2014, Two-photon fluorescence (TPF) microscopy of corneal riboflavin absorption, Publisher: ASSOC RESEARCH VISION OPHTHALMOLOGY INC, ISSN: 0146-0404

Conference paper

sparks H, warren S, Guedes J, Yoshida N, Charn TC, Guerra N, Tatla T, dunsby C, french Pet al., A flexible wide-field FLIM endoscope utilising blue excitation light for label-free contrast of tissue., Journal of Biophotonics, Vol: 8, Pages: 168-178, ISSN: 1864-0648

Journal article

Coda S, Thompson AJ, Kennedy GT, Roche KL, Ayaru L, Bansi DS, Stamp GW, Thillainayagam AV, French PMW, Dunsby Cet al., 2014, Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe, Biomedical Optics Express, Vol: 5, Pages: 515-538

We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime −570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens.

Journal article

Campagnola P, French PMW, Georgakoudi I, Mycek M-Aet al., 2014, Introduction: feature issue on optical molecular probes, imaging, and drug delivery, BIOMEDICAL OPTICS EXPRESS, Vol: 5, Pages: 643-644, ISSN: 2156-7085

Journal article

Coda S, French PMW, Dunsby C, 2014, Oncology applications: Gastrointestinal cancer, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics, Pages: 379-386, ISBN: 9781439861677

© 2015 by Taylor & Francis Group, LLC. Cancers of esophagus, stomach, and colon are among the most common cancers worldwide, accounting for a total of 2.2 million new cases each year (Boyle and Levin 2008). Prevention of these conditions is currently based on early detection of early-stage cancers or premalignant conditions during conventional white-light endoscopy (WLE). Today, there is a range of more sophisticated biophotonics techniques under development that aim to enhance the contrast of areas of concern beyond what is possible with conventional WLE. Commercially available techniques include high-definition endoscopy (HDE; Adler et al. 2009; Buchner 2010; Rex and Helbig 2007), narrow band imaging (NBI; Gono et al. 2004), magnifying chromoendoscopy (MCE; Kudo et al. 1996), autofluorescence (AF) imaging (AFI; Nakaniwa et al. 2005), and confocal laser endomicroscopy (CLE; Kiesslich et al. 2004; Wang et al. 2007).

Book chapter

Elson DS, Marcu L, French PMW, 2014, Overview of fluorescence lifetime imaging and metrology, ISBN: 9781439861677

© 2015 by Taylor & Francis Group, LLC. This chapter aims to present an overview of fluorescence lifetime imaging (FLIM) and metrology in the context of their biomedical applications, introducing the main approaches that are discussed in detail in subsequent chapters of this book. Before discussing fluorescence lifetime measurements, however, it is important to understand the phenomenon of fluorescence, of which a brief discussion is provided here, and the reader is directed to the classic textbook by Lakowicz (1999) for further details.

Book

French PMW, 2014, Fluorescence lifetime imaging for biomedicine

I will review our development and application of fluorescence lifetime imaging implemented in microscopy, tomography and endoscopy to provide molecular readouts across the scales from super-resolved microscopy through imaging of disease models to clinical applications. © 2014 OSA.

Conference paper

Nickdel MB, Lagarto JL, Kelly DJ, Manning HB, Yamamoto K, Talbot CB, Dunsby C, French P, Itoh Yet al., 2014, Autofluorescence lifetime metrology for label-free detection of cartilage matrix degradation, Conference on Optical Biopsy XII, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

Conference paper

Sonnefraud Y, Sivan Y, Sinclair HG, Dunsby CW, Neil MA, French PM, Maier SAet al., 2014, Nanoparticle-assisted STED, theory, and experimental demonstration, Conference on Nanoimaging and Nanospectroscopy II, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

Conference paper

Marcu L, French PMW, Elson DS, 2014, Preface, ISBN: 9781439861677

© 2015 by Taylor & Francis Group, LLC. Wide-field time-gated fluorescence lifetime imaging (FLIM) essentially entails illuminating a sample with an ultrashort pulse of excitation radiation and sampling the resulting time varying fluorescence “image” following excitation by acquiring a series of gated fluorescence intensity images recorded at different relative delays with respect to the excitation pulse. This is represented schematically in Figure 8.1. In the simplest case, a map of the mean fluorescence decay times across the field of view is obtained. If the sampling of the fluorescence decay profiles is appropriately detailed, then the entire fluorescence decay profile for each image pixel can be acquired, and the resulting data set can be fitted to complex temporal decay models. For example, a double exponential decay model is frequently used to analyze data from Förster resonant energy transfer (FRET) experiments. The acquisition of time-gated fluorescence intensity images requires a 2-D detector, normally a charge-coupled device (CCD) camera, and some kind of fast “shutter” able to sample fluorescence decay profiles on subnanosecond timescales. Such a “shutter” function cannot be provided by mechanical means or yet by electronic circuitry and is typically provided by optical image intensifiers whose gain can be modulated by varying the applied voltage.

Book

French PMW, 2014, Overview of fluorescence imaging for biophotonics, 181st International School of Physics Enrico Fermi on Microscopy Applied to Biophotonics, Publisher: IOS PRESS, Pages: 1-27, ISSN: 0074-784X

Conference paper

Dunsby C, McGinty J, French P, 2014, Multidimensional fluorescence imaging of biological tissue, Biomedical Photonics Handbook, Second Edition: Fundamentals, Devices, and Techniques, Pages: 531-560, ISBN: 9781420085129

© 2015 by Taylor & Francis Group, LLC. This chapter aims to review multidimensional fluorescence imaging (MDFI) technology and its application to biological tissue, with a particular emphasis on fluorescence lifetime imaging (FLIM) of biological tissue with examples from our work at Imperial College London. Fluorescence imaging is flourishing tremendously, partly driven by advances in laser and detector technology, partly by advances in labeling technologies such as genetically expressed fluorescent proteins, and partly by advances in computational analysis techniques. Increasingly, fluorescence instrumentation is developed to provide more information than just the localization or distribution of specific fluorescent molecules. Often, fluorescence signals are analyzed to provide information on the local fluorophore environment or to contrast different fluorophores in complex mixtures-as often occur in biological tissue. This trend to higher-content fluorescence imaging increasingly exploits MDFI and measurement capabilities with instrumentation that resolves fluorescence lifetime together with other spectroscopic parameters such as excitation and emission wavelength and polarization, providing image information in two or three spatial dimensions as well as with respect to elapsed time (Figure 18.1). However, caution should be exercised when acquiring such MDFI since photobleaching or experimental considerations usually impose a limited photon budget and/or a maximum image acquisition time and also present significant challenges with respect to data analysis and data management. These considerations are particularly important for real-time clinical diagnostic applications, for higher-throughput assays, and for the investigation of dynamic biological systems (Figure 18.1).

Book chapter

Lenz MO, Sinclair HG, Savell A, Clegg JH, Brown ACN, Davis DM, Dunsby C, Neil MAA, French PMWet al., 2014, 3-D stimulated emission depletion microscopy with programmable aberration correction, JOURNAL OF BIOPHOTONICS, Vol: 7, Pages: 29-36, ISSN: 1864-063X

Journal article

French PMW, 2014, Fluorescence Lifetime Imaging for Biomedicine, 2014 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO), ISSN: 2160-9020

Journal article

Xavier GDS, Bellomo EA, McGinty JA, French PM, Rutter GAet al., 2013, Animal Models of GWAS-Identified Type 2 Diabetes Genes, Journal of Diabetes Research, Vol: 2013, ISSN: 2314-6753

More than 65 loci, encoding up to 500 different genes, have been implicated by genome-wide association studies (GWAS) as conferring an increased risk of developing type 2 diabetes (T2D). Whilst mouse models have in the past been central to understanding the mechanisms through which more penetrant risk genes for T2D, for example, those responsible for neonatal or maturity-onset diabetes of the young, only a few of those identified by GWAS, notably TCF7L2 and ZnT8/SLC30A8, have to date been examined in mouse models. We discuss here the animal models available for the latter genes and provide perspectives for future, higher throughput approaches towards efficiently mining the information provided by human genetics.

Journal article

Myatt SS, Kongsema M, Man CW-Y, Kelly DJ, Gomes AR, Khongkow P, Karunarathna U, Zona S, Langer JK, Dunsby CW, Coombes RC, French PM, Brosens JJ, Lam EW-Fet al., 2013, SUMOylation inhibits FOXM1 activity and delays mitotic transition, Oncogene, Vol: 33, Pages: 4316-4329, ISSN: 1476-5594

The forkhead box transcription factor FOXM1 is an essential effector of G2/M-phase transition, mitosis and the DNA damage response. As such, it is frequently deregulated during tumorigenesis. Here we report that FOXM1 is dynamically modified by SUMO1 but not by SUMO2/3 at multiple sites. We show that FOXM1 SUMOylation is enhanced in MCF-7 breast cancer cells in response to treatment with epirubicin and mitotic inhibitors. Mutation of five consensus conjugation motifs yielded a SUMOylation-deficient mutant FOXM1. Conversely, fusion of the E2 ligase Ubc9 to FOXM1 generated an auto-SUMOylating mutant (FOXM1-Ubc9). Analysis of wild-type FOXM1 and mutants revealed that SUMOylation inhibits FOXM1 activity, promotes translocation to the cytoplasm and enhances APC/Cdh1-mediated ubiquitination and degradation. Further, expression of the SUMOylation-deficient mutant enhanced cell proliferation compared with wild-type FOXM1, whereas the FOXM1-Ubc9 fusion protein resulted in persistent cyclin B1 expression and slowed the time from mitotic entry to exit. In summary, our findings suggest that SUMOylation attenuates FOXM1 activity and causes mitotic delay in cytotoxic drug response.

Journal article

Kelly DJ, Alibhai D, Warren S, Kumar S, Margineanu A, Stuhmeier F, Murray EJ, Katan M, Lam EWF, Neil MAA, Dunsby C, French PMWet al., 2013, An automated flim multiwell plate reader for high content analysis

We report an automated fluorescence lifetime imaging multiwell plate reader for high content analysis, capable of subcellular mapping of protein interactions. This instrument can acquire FLIM data from 96 wells in less than 15 minutes.©2013 The Optical Society (OSA).

Conference paper

Warren S, Kimberley C, Margineanu A, Laine R, Dunsby C, Katan M, French Pet al., 2013, Flim-fret of cell signalling in chemotaxis

We demonstrate the application of Fluorescence Lifetime Imaging (FLIM) to read out Förster resonant energy transfer (FRET) based biosensors for studying the spatio-temporal dynamics of signalling pathways in cells undergoing chemotaxis. ©2013 The Optical Society (OSA).

Conference paper

Coda S, Kelly DJ, Lagarto JL, Manning HB, Patalay R, Sparks H, Thompson AJ, Warren SC, Dudhia J, Kennedy G, Nickdel MB, Talbot CB, Yamamoto K, Neil MAA, Itoh Y, McGinty J, Stamp GW, Thillainayagam AV, Dunsby C, French PMWet al., 2013, Autofluorescence lifetime imaging and metrology for medical research and clinical diagnosis

We report the development of instrumentation to utilise autofluorescence lifetime for the study and diagnosis of disease including cancer and osteoarthritis. ©2013 The Optical Society (OSA).

Conference paper

Roper JC, Yerolatsitis S, Birks TA, Mangan BJ, Dunsby C, French PMW, Knight JCet al., 2013, Minimising group index variations in a multicore endoscope fibre

We describe a multicore endoscope fibre with minimised group index variation between cores that is obtained at a V parameter of 3. Tapering the fibre input enables us to achieve single-mode propagation. © OSA 2013.

Conference paper

Nickdel MB, Lagarto JL, Kelly DJ, Manning HB, Yamamoto K, Talbot CB, Dudhia J, Dunsby C, French PM, Itoh Yet al., 2013, Detection of cartilage matrix degradation by autofluorescence lifetime, Spring Meeting of the British-Society-for-Matrix-Biology, Publisher: WILEY-BLACKWELL, Pages: A12-A13, ISSN: 0959-9673

Conference paper

Xavier GDS, Mondragon A, Mitchell R, Hodson DJ, Ferre J, Thorens B, McGinty JA, French PMW, Rutter GAet al., 2013, Defective glucose homeostasis in mice inactivated selectively for Tcf7l2 in the adult beta cell with an Ins1-controlled Cre, 49th Annual Meeting of the European-Association-for-the-Study-of-Diabetes (EASD), Publisher: SPRINGER, Pages: S142-S142, ISSN: 0012-186X

Conference paper

Warren SC, Margineanu A, Alibhai D, Kelly DJ, Talbot C, Alexandrov Y, Munro I, Katan M, Dunsby C, French PMWet al., 2013, Rapid Global Fitting of Large Fluorescence Lifetime Imaging Microscopy Datasets, PLOS ONE, Vol: 8, ISSN: 1932-6203

Journal article

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