481 results found
Dunsby C, French PMW, 2010, Biophotonics applications of supercontinuum generation, Supercontinuum Generation in Optical Fibers, Pages: 349-373, ISBN: 9780521514804
© Cambridge University Press 2010. Introduction Continua generated using high pulse energy laser systems to create broad spectra (Alfano and Shapiro, 1970) have been used for spectroscopy for many years (e.g. Busch et al., 1973). The application of fibre-generated continua to spectroscopy was suggested as early as 1976 in work by Lin and Stolen (1976) where a continuum spanning ∼450-600 nm was generated in a step-index fibre pumped by a nitrogen pumped dye laser. Since the demonstration of supercontinuum generation in microstructured optical fibres (MOF), however, the range of spectroscopic and imaging applications has increased enormously, owing to the high average powers, unprecedented spectral width and relatively low cost and low complexity of such sources. This chapter specifically focuses on the applications of supercontinua generated in MOFs and, in particular, on applications in biophotonics. MOF supercontinuum sources can be broadly grouped into three categories according to whether the laser pump source emits femtosecond pulses, picosecond-nanosecond pulses or cw radiation. In general terms, sub-ps pulses can produce broad supercontinua spanning from the UV to the NIR but the peak intensity damage threshold at the input end of the microstructured optical fibre limits the maximum average power that can be obtained in the supercontinuum to ∼<0.5 W with typically ∼<0.5 mW/nm available in the visible spectrum. The use of pump lasers with longer ps-ns pulses can significantly increase the maximum available average power before the onset of damage in the MOF such that high power supercontinua with several mW/nm in the visible can be achieved (e.g. Rulkov et al., 2005).
Kennedy GT, Manning HB, Elson DS, et al., 2010, A fluorescence lifetime imaging scanning confocal endomicroscope, JOURNAL OF BIOPHOTONICS, Vol: 3, Pages: 103-107, ISSN: 1864-063X
DeMello AJ, French PMW, Neil MAA, et al., 2009, Optical detection in microfluidics: From the small to the large, Pages: 712-717
Herein we discuss two broad approaches for performing high sensitivity optical detection within microfluidic environments. First, we describe recent work in which fluorescence lifetime imaging has been shown to be a sensitive probe of environmental parameters such as pH, viscosity, molecular concentration and temperature. Additionally, we demonstrate how dynamic fluorescence lifetime imaging can be used to probe mixing dynamics in segmented-flow microfluidic systems. Moreover, we describe recent work at Imperial College London in which semiconducting polymer light emitting diodes and polymer photodetectors are integrated with microfluidic systems to define a novel format for point-of-care diagnostics. ©2009 IEEE.
McGinty J, Soloviev VY, Tahir KB, et al., 2009, Three-dimensional imaging of Forster resonance energy transfer in heterogeneous turbid media by tomographic fluorescent lifetime imaging, OPT LETT, Vol: 34, Pages: 2772-2774, ISSN: 0146-9592
We report a three-dimensional time-resolved tomographic imaging technique for localizing protein-protein interaction and protein conformational changes in turbid media based on Forster resonant energy-transfer read out using fluorescence lifetime. This application of "tomoFRET" employs an inverse scattering algorithm utilizing the diffusion approximation to the radiative-transfer equation applied to a large tomographic data set of time-gated images. The approach is demonstrated by imaging a highly scattering cylindrical phantom within which are two thin wells containing cytosol preparations of HEK293 cells expressing TN-L15, a cytosolic genetically encoded calcium Forster resonant energy-transfer sensor. A 10 mM calcium chloride solution was added to one of the wells, inducing a protein conformation change upon binding to TN-L15, resulting in Forster resonant energy transfer and a corresponding decrease in the donor fluorescence lifetime. We successfully reconstruct spatially resolved maps of the resulting fluorescence lifetime distribution as well as of the quantum efficiency, absorption, and scattering coefficients. (C) 2009 Optical Society of America
McGinty J, Requejo-Isidro J, Munro I, et al., 2009, Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 42, ISSN: 0022-3727
Galletly N, McGinty J, Munro I, et al., 2009, Fluorescence lifetime imaging of liver cancer, 107th Annual Meeting of the American-Gastroenterlogical Association, Publisher: W B Saunders Co-Elsevier Inc
Benninger RKP, Vanherberghen B, Young S, et al., 2009, Live Cell Linear Dichroism Imaging Reveals Extensive Membrane Ruffling within the Docking Structure of Natural Killer Cell Immune Synapses, BIOPHYSICAL JOURNAL, Vol: 96, Pages: L13-L15, ISSN: 0006-3495
Schaerli Y, Wootton RC, Robinson T, et al., 2009, Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets, ANALYTICAL CHEMISTRY, Vol: 81, Pages: 302-306, ISSN: 0003-2700
Robinson T, Schaerli Y, Wootton R, et al., 2009, Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging, LAB ON A CHIP, Vol: 9, Pages: 3437-3441, ISSN: 1473-0197
McGinty J, Dunsby C, Auksorius E, et al., 2009, Multidimensional fluorescence imaging, FRET and FLIM Techniques, ISBN: 0080549586
McGinty J, Dunsby C, Auksorius E, et al., 2009, Multidimensional fluorescence imaging, Laboratory Techniques in Biochemistry and Molecular Biology (FLIM and FRET techniques), Editors: Gadella, Publisher: Elsevier, ISBN: 9780080915128
This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips.
Talbot C, McGinty J, McGhee E, et al., 2008, High speed, optically sectioned fluorescence lifetime imaging utilizing time-gated nipkow disk or multifocal multiphoton time correlated single photon counting microscopy
We report two optically sectioned fluorescence lifetime systems that exhibit better signal to noise per unit time than conventional time correlated single photon counting systems. Both systems are applied to biologically relevant samples. ©2007 Optical Society of America.
Manning HB, Kennedy GT, Owen DM, et al., 2008, A compact, multidimensional spectrofluorometer exploiting supercontinuum generation, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 494-505, ISSN: 1864-063X
McGinty J, Tahir KB, Soloviev VY, et al., 2008, Time-domain fluorescence lifetime tomography
We present a platform for fluorescence lifetime tomography utilising tuneable supercontinuum excitation and wide-field time-gated technology. Applied to optical projection and diffuse fluorescence tomography, we demonstrate 3D time-resolved fluorescence reconstruction in transparent and scattering phantoms.
Talbot CB, McGinty J, Grant DM, et al., 2008, High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 514-521, ISSN: 1864-063X
Grant DM, Zhang W, McGhee EJ, et al., 2008, Multiplexed FRET to Image Multiple Signaling Events in Live Cells, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L69-L71, ISSN: 0006-3495
Oddos S, Dunsby C, Purbhoo MA, et al., 2008, High-Speed High-Resolution Imaging of Intercellular Immune Synapses Using Optical Tweezers, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L66-L68, ISSN: 0006-3495
Lanigan PMP, Chan K, Ninkovic T, et al., 2008, Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 5, Pages: S161-S168, ISSN: 1742-5689
McGinty J, Tahir KR, Laine R, et al., 2008, Fluorescence lifetime optical projection tomography, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 390-394, ISSN: 1864-063X
Robinson T, Valluri P, Manning HB, et al., 2008, Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 1887-1889, ISSN: 0146-9592
Poher V, Kennedy GT, Manning HB, et al., 2008, Improved sectioning in a slit scanning confocal microscope, OPTICS LETTERS, Vol: 33, Pages: 1813-1815, ISSN: 0146-9592
Galletly NP, McGinty J, Dunsby C, et al., 2008, Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin, BRITISH JOURNAL OF DERMATOLOGY, Vol: 159, Pages: 152-161, ISSN: 0007-0963
Poher V, Grossman N, Kennedy GT, et al., 2008, Micro-LED arrays: a tool for two-dimensional neuron stimulation, Journal of Physics D: Applied Physics, Vol: 41, Pages: 094014-094023, ISSN: 0022-3727
Gong Z, Gu E, Jin SR, et al., 2008, Efficient flip-chip InGaN micro-pixellated light-emitting diode arrays: Promising candidates for micro-displays and colour conversion, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 41, ISSN: 0022-3727
Kennedy GT, Elson DS, Hares JD, et al., 2008, Fluorescence lifetime imaging using light emitting diodes, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 41, ISSN: 0022-3727
Auksorius E, Boruah BR, Dunsby C, et al., 2008, Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 113-115, ISSN: 0146-9592
De Beule PAA, Dunsby C, Galletly NP, et al., 2007, A hyperspectral fluorescence lifetime probe for skin cancer diagnosis, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 78, ISSN: 0034-6748
Owen DM, Auksorius E, Manning HB, et al., 2007, Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source, OPTICS LETTERS, Vol: 32, Pages: 3408-3410, ISSN: 0146-9592
Grant DM, McGinty J, McGhee EJ, et al., 2007, High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events, Optics Express, Vol: 15, Pages: 16656-16673
We present a time domain optically sectioned fluorescence lifetime imaging (FLIM) microscope developed for high-speed live cell imaging. This single photon excited system combines wide field parallel pixel detection with confocal sectioning utilizing spinning Nipkow disc microscopy. It can acquire fluorescence lifetime images of live cells at up to 10 frames per second (fps), permitting high-speed FLIM of cell dynamics and protein interactions with potential for high throughput cell imaging and screening applications. We demonstrate the application of this FLIM microscope to real-time monitoring of changes in lipid order in cell membranes following cholesterol depletion using cyclodextrin and to the activation of the small GTP-ase Ras in live cells using FRET. © 2007 Optical Society of America.
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