Imperial College London

ProfessorPaulFrench

Faculty of Natural SciencesDepartment of Physics

Professor of Physics and Vice Dean (Research) - FoNS
 
 
 
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Contact

 

+44 (0)20 7594 7706paul.french Website

 
 
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Assistant

 

Ms Judith Baylis +44 (0)20 7594 7713

 
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Location

 

609Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

481 results found

Dunsby C, French PMW, 2010, Biophotonics applications of supercontinuum generation, Supercontinuum Generation in Optical Fibers, Pages: 349-373, ISBN: 9780521514804

© Cambridge University Press 2010. Introduction Continua generated using high pulse energy laser systems to create broad spectra (Alfano and Shapiro, 1970) have been used for spectroscopy for many years (e.g. Busch et al., 1973). The application of fibre-generated continua to spectroscopy was suggested as early as 1976 in work by Lin and Stolen (1976) where a continuum spanning ∼450-600 nm was generated in a step-index fibre pumped by a nitrogen pumped dye laser. Since the demonstration of supercontinuum generation in microstructured optical fibres (MOF), however, the range of spectroscopic and imaging applications has increased enormously, owing to the high average powers, unprecedented spectral width and relatively low cost and low complexity of such sources. This chapter specifically focuses on the applications of supercontinua generated in MOFs and, in particular, on applications in biophotonics. MOF supercontinuum sources can be broadly grouped into three categories according to whether the laser pump source emits femtosecond pulses, picosecond-nanosecond pulses or cw radiation. In general terms, sub-ps pulses can produce broad supercontinua spanning from the UV to the NIR but the peak intensity damage threshold at the input end of the microstructured optical fibre limits the maximum average power that can be obtained in the supercontinuum to ∼<0.5 W with typically ∼<0.5 mW/nm available in the visible spectrum. The use of pump lasers with longer ps-ns pulses can significantly increase the maximum available average power before the onset of damage in the MOF such that high power supercontinua with several mW/nm in the visible can be achieved (e.g. Rulkov et al., 2005).

Book chapter

Kennedy GT, Manning HB, Elson DS, Neil MAA, Stamp GW, Viellerobe B, Lacombe F, Dunsby C, French PMWet al., 2010, A fluorescence lifetime imaging scanning confocal endomicroscope, JOURNAL OF BIOPHOTONICS, Vol: 3, Pages: 103-107, ISSN: 1864-063X

Journal article

DeMello AJ, French PMW, Neil MAA, Edel JB, Benninger RKP, Bradley DDC, Robinson T, Srisa-art M, Hofmann O, DeMello JCet al., 2009, Optical detection in microfluidics: From the small to the large, Pages: 712-717

Herein we discuss two broad approaches for performing high sensitivity optical detection within microfluidic environments. First, we describe recent work in which fluorescence lifetime imaging has been shown to be a sensitive probe of environmental parameters such as pH, viscosity, molecular concentration and temperature. Additionally, we demonstrate how dynamic fluorescence lifetime imaging can be used to probe mixing dynamics in segmented-flow microfluidic systems. Moreover, we describe recent work at Imperial College London in which semiconducting polymer light emitting diodes and polymer photodetectors are integrated with microfluidic systems to define a novel format for point-of-care diagnostics. ©2009 IEEE.

Conference paper

McGinty J, Soloviev VY, Tahir KB, Laine R, Stuckey DW, Hajnal JV, Sardini A, PMW F, Arridge SRet al., 2009, Three-dimensional imaging of Forster resonance energy transfer in heterogeneous turbid media by tomographic fluorescent lifetime imaging, OPT LETT, Vol: 34, Pages: 2772-2774, ISSN: 0146-9592

We report a three-dimensional time-resolved tomographic imaging technique for localizing protein-protein interaction and protein conformational changes in turbid media based on Forster resonant energy-transfer read out using fluorescence lifetime. This application of "tomoFRET" employs an inverse scattering algorithm utilizing the diffusion approximation to the radiative-transfer equation applied to a large tomographic data set of time-gated images. The approach is demonstrated by imaging a highly scattering cylindrical phantom within which are two thin wells containing cytosol preparations of HEK293 cells expressing TN-L15, a cytosolic genetically encoded calcium Forster resonant energy-transfer sensor. A 10 mM calcium chloride solution was added to one of the wells, inducing a protein conformation change upon binding to TN-L15, resulting in Forster resonant energy transfer and a corresponding decrease in the donor fluorescence lifetime. We successfully reconstruct spatially resolved maps of the resulting fluorescence lifetime distribution as well as of the quantum efficiency, absorption, and scattering coefficients. (C) 2009 Optical Society of America

Journal article

McGinty J, Requejo-Isidro J, Munro I, Talbot CB, Kellett PA, Hares JD, Dunsby C, Neil MAA, French PMWet al., 2009, Signal-to-noise characterization of time-gated intensifiers used for wide-field time-domain FLIM, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 42, ISSN: 0022-3727

Journal article

Galletly N, McGinty J, Munro I, Elson DS, Requejo-Isidro J, Dunsby C, Neil MA, Thillainayagam AV, French PM, Stamp GWet al., 2009, Fluorescence lifetime imaging of liver cancer, 107th Annual Meeting of the American-Gastroenterlogical Association, Publisher: W B Saunders Co-Elsevier Inc

Conference paper

McGinty J, Dunsby C, Auksorius E, Benninger RKP, De Beule P, Elson DS, Galletly N, Grant D, Hofmann O, Kennedy G, Kumar S, Lanigan PMP, Manning H, Munro I, Önfelt B, Owen D, Requejo-Isidro J, Suhling K, Talbot CB, Soutter P, Lever MJ, deMello AJ, Stamp GS, Neil MAA, French PMWet al., 2009, Chapter 4 Multidimensional fluorescence imaging

Book

Benninger RKP, Vanherberghen B, Young S, Taner SB, Culley FI, Schnyder T, Neil MAA, Wuestner D, French PMW, Davis DM, Onfelt Bet al., 2009, Live Cell Linear Dichroism Imaging Reveals Extensive Membrane Ruffling within the Docking Structure of Natural Killer Cell Immune Synapses, BIOPHYSICAL JOURNAL, Vol: 96, Pages: L13-L15, ISSN: 0006-3495

Journal article

Schaerli Y, Wootton RC, Robinson T, Stein V, Dunsby C, Neil MAA, French PMW, deMello AJ, Abell C, Hollfelder Fet al., 2009, Continuous-Flow Polymerase Chain Reaction of Single-Copy DNA in Microfluidic Microdroplets, ANALYTICAL CHEMISTRY, Vol: 81, Pages: 302-306, ISSN: 0003-2700

Journal article

Robinson T, Schaerli Y, Wootton R, Hollfelder F, Dunsby C, Baldwin G, Neil M, French P, deMello Aet al., 2009, Removal of background signals from fluorescence thermometry measurements in PDMS microchannels using fluorescence lifetime imaging, LAB ON A CHIP, Vol: 9, Pages: 3437-3441, ISSN: 1473-0197

Journal article

McGinty J, Dunsby C, Auksorius E, Benninger RKP, De Beule PAA, Elson DS, Galletly NP, Hofmann O, Kennedy G, Lanigan PMP, Munro I, Onfelt B, Requejo-Isidro J, Suhling K, Talbot CB, Soutter P, Lever MJ, deMello AJ, Stamp GWH, Neil MAA, French PMWet al., 2009, Multidimensional fluorescence imaging, FRET and FLIM Techniques, ISBN: 0080549586

Book chapter

McGinty J, Dunsby C, Auksorius E, Benninger RKP, De Beule P, Elson DS, Galletly N, Hofmann O, Kennedy G, Lanigan P, Munro I, Onfelt B, Requejo-Isidro J, Suhling K, Talbot C, Soutter P, Lever M, deMello AJ, Stamp G, Neil M, French Pet al., 2009, Multidimensional fluorescence imaging, Laboratory Techniques in Biochemistry and Molecular Biology (FLIM and FRET techniques), Editors: Gadella, Publisher: Elsevier, ISBN: 9780080915128

This volume reviews the techniques Förster Resonance Energy Transfer (FRET) and Fluorescence Lifetime Imaging Microscopy (FLIM) providing researchers with step by step protocols and handy hints and tips.

Book chapter

Talbot C, McGinty J, McGhee E, Grant D, Kumar S, Owen D, Kennedy G, Munro I, Courtney P, Zhang W, Bunney T, Magee T, Davis D, Katan M, Dunsby C, Neil M, French Pet al., 2008, High speed, optically sectioned fluorescence lifetime imaging utilizing time-gated nipkow disk or multifocal multiphoton time correlated single photon counting microscopy

We report two optically sectioned fluorescence lifetime systems that exhibit better signal to noise per unit time than conventional time correlated single photon counting systems. Both systems are applied to biologically relevant samples. ©2007 Optical Society of America.

Conference paper

Manning HB, Kennedy GT, Owen DM, Grant DM, Magee AI, Neil MAA, Itoh Y, Dunsby C, French PMWet al., 2008, A compact, multidimensional spectrofluorometer exploiting supercontinuum generation, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 494-505, ISSN: 1864-063X

Journal article

McGinty J, Tahir KB, Soloviev VY, Laine R, Sardini A, Talbot CB, Dunsby C, Munro I, Elson DS, Hajnal JV, Neil MAA, Arridge SR, French PMWet al., 2008, Time-domain fluorescence lifetime tomography

We present a platform for fluorescence lifetime tomography utilising tuneable supercontinuum excitation and wide-field time-gated technology. Applied to optical projection and diffuse fluorescence tomography, we demonstrate 3D time-resolved fluorescence reconstruction in transparent and scattering phantoms.

Conference paper

Talbot CB, McGinty J, Grant DM, McGhee EJ, Owen DM, Zhang W, Bunney TD, Munro I, Isherwood B, Eagle R, Hargreaves A, Katan M, Dunsby C, Neil MAA, French PMWet al., 2008, High speed unsupervised fluorescence lifetime imaging confocal multiwell plate reader for high content analysis, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 514-521, ISSN: 1864-063X

Journal article

Grant DM, Zhang W, McGhee EJ, Bunney TD, Talbot CB, Kumar S, Munro I, Dunsby C, Neil MAA, Katan M, French PMWet al., 2008, Multiplexed FRET to Image Multiple Signaling Events in Live Cells, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L69-L71, ISSN: 0006-3495

Journal article

Oddos S, Dunsby C, Purbhoo MA, Chauveau A, Owen DM, Neil MAA, Davis DM, French PMWet al., 2008, High-Speed High-Resolution Imaging of Intercellular Immune Synapses Using Optical Tweezers, BIOPHYSICAL JOURNAL, Vol: 95, Pages: L66-L68, ISSN: 0006-3495

Journal article

Lanigan PMP, Chan K, Ninkovic T, Templer RH, French PMW, de Mello AJ, Willison KR, Parker PJ, Neil MAA, Ces O, Klug DRet al., 2008, Spatially selective sampling of single cells using optically trapped fusogenic emulsion droplets: a new single-cell proteomic tool, JOURNAL OF THE ROYAL SOCIETY INTERFACE, Vol: 5, Pages: S161-S168, ISSN: 1742-5689

Journal article

McGinty J, Tahir KR, Laine R, Talbot CB, Dunsby C, Neil MAA, Quintana L, Swoger J, Sharpe J, French PMWet al., 2008, Fluorescence lifetime optical projection tomography, JOURNAL OF BIOPHOTONICS, Vol: 1, Pages: 390-394, ISSN: 1864-063X

Journal article

Robinson T, Valluri P, Manning HB, Owen DM, Munro I, Talbot CB, Dunsby C, Eccleston JF, Baldwin GS, Neil MAA, de Mello AJ, French PMWet al., 2008, Three-dimensional molecular mapping in a microfluidic mixing device using fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 1887-1889, ISSN: 0146-9592

Journal article

Poher V, Kennedy GT, Manning HB, Owen DM, Zhang HX, Gu E, Dawson MD, French PMW, Neil MAAet al., 2008, Improved sectioning in a slit scanning confocal microscope, OPTICS LETTERS, Vol: 33, Pages: 1813-1815, ISSN: 0146-9592

Journal article

Galletly NP, McGinty J, Dunsby C, Teixeira F, Requejo-Isidro J, Munro I, Elson DS, Neil MAA, Chu AC, French PMW, Stamp GWet al., 2008, Fluorescence lifetime imaging distinguishes basal cell carcinoma from surrounding uninvolved skin, BRITISH JOURNAL OF DERMATOLOGY, Vol: 159, Pages: 152-161, ISSN: 0007-0963

Journal article

Poher V, Grossman N, Kennedy GT, Nikolic K, Zhang HX, Gong Z, Drakakis EM, Gu E, Dawson MD, French PMW, Degenaar P, Neil MAAet al., 2008, Micro-LED arrays: a tool for two-dimensional neuron stimulation, Journal of Physics D: Applied Physics, Vol: 41, Pages: 094014-094023, ISSN: 0022-3727

Journal article

Gong Z, Gu E, Jin SR, Massoubre D, Guilhabert B, Zhang HX, Dawson MD, Poher V, Kennedy GT, French PMW, Neil MAAet al., 2008, Efficient flip-chip InGaN micro-pixellated light-emitting diode arrays: Promising candidates for micro-displays and colour conversion, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 41, ISSN: 0022-3727

Journal article

Kennedy GT, Elson DS, Hares JD, Munro I, Poher V, French PMW, Neil MAAet al., 2008, Fluorescence lifetime imaging using light emitting diodes, JOURNAL OF PHYSICS D-APPLIED PHYSICS, Vol: 41, ISSN: 0022-3727

Journal article

Auksorius E, Boruah BR, Dunsby C, Lanigan PMP, Kennedy G, Neil MAA, French PMWet al., 2008, Stimulated emission depletion microscopy with a supercontinuum source and fluorescence lifetime imaging, OPTICS LETTERS, Vol: 33, Pages: 113-115, ISSN: 0146-9592

Journal article

De Beule PAA, Dunsby C, Galletly NP, Stamp GW, Chu AC, Anand U, Anand P, Benham CD, Naylor A, French PMWet al., 2007, A hyperspectral fluorescence lifetime probe for skin cancer diagnosis, REVIEW OF SCIENTIFIC INSTRUMENTS, Vol: 78, ISSN: 0034-6748

Journal article

Owen DM, Auksorius E, Manning HB, Talbot CB, de Beule PAA, Dunsby C, Neil MAA, French PMWet al., 2007, Excitation-resolved hyperspectral fluorescence lifetime imaging using a UV-extended supercontinuum source, OPTICS LETTERS, Vol: 32, Pages: 3408-3410, ISSN: 0146-9592

Journal article

Grant DM, McGinty J, McGhee EJ, Bunney TD, Owen DM, Talbot CB, Zhang W, Kumar S, Munro I, Lanigan PMP, Kennedy GT, Dunsby C, Magee AI, Courtney P, Katan M, Neil MAA, French PMWet al., 2007, High speed optically sectioned fluorescence lifetime imaging permits study of live cell signaling events, Optics Express, Vol: 15, Pages: 16656-16673

We present a time domain optically sectioned fluorescence lifetime imaging (FLIM) microscope developed for high-speed live cell imaging. This single photon excited system combines wide field parallel pixel detection with confocal sectioning utilizing spinning Nipkow disc microscopy. It can acquire fluorescence lifetime images of live cells at up to 10 frames per second (fps), permitting high-speed FLIM of cell dynamics and protein interactions with potential for high throughput cell imaging and screening applications. We demonstrate the application of this FLIM microscope to real-time monitoring of changes in lipid order in cell membranes following cholesterol depletion using cyclodextrin and to the activation of the small GTP-ase Ras in live cells using FRET. © 2007 Optical Society of America.

Journal article

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