Imperial College London

ProfessorPaulFrench

Faculty of Natural SciencesDepartment of Physics

Professor of Physics and Vice Dean (Research) - FoNS
 
 
 
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Contact

 

+44 (0)20 7594 7706paul.french Website

 
 
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Assistant

 

Ms Judith Baylis +44 (0)20 7594 7713

 
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Location

 

609Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Publication Type
Year
to

478 results found

Benninger RK, Hofmann O, Onfelt B, Munro I, Dunsby C, Davis DM, Neil MA, French PM, de Mello AJet al., 2007, Fluorescence-Lifetime Imaging of DNA-Dye Interactions within Continuous-Flow Microfluidic Systems., Angew Chem Int Ed Engl, Vol: 46, Pages: 8536-8536, ISSN: 1433-7851

JOURNAL ARTICLE

Soloviev VY, Tahir KB, McGinty J, Elson DS, Neil MAA, French PMW, Arridge SRet al., 2007, Fluorescence lifetime imaging by using time-gated data acquisition, APPLIED OPTICS, Vol: 46, Pages: 7384-7391, ISSN: 1559-128X

JOURNAL ARTICLE

Gong Z, Zhang HX, Gu E, Griffin C, Dawson MD, Poher V, Kennedy G, French PMW, Neil MAAet al., 2007, Matrix-addressable micropixellated InGaN light-emitting diodes with uniform emission and increased light output, IEEE TRANSACTIONS ON ELECTRON DEVICES, Vol: 54, Pages: 2650-2658, ISSN: 0018-9383

JOURNAL ARTICLE

Owen DM, Neil MAA, French PMW, Magee AIet al., 2007, Optical techniques for imaging membrane lipid microdomains in living cells, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, Vol: 18, Pages: 591-598, ISSN: 1084-9521

JOURNAL ARTICLE

Kumar S, Dunsby C, De Beule PAA, Owen DM, Anand U, Lanigan PMP, Benninger RKP, Davis DM, Neil MAA, Anand P, Benham C, Naylor A, French PMWet al., 2007, Multifocal multiphoton excitation and time correlated single photon counting detection for 3-D fluorescence lifetime imaging, Optics Express, Vol: 15, Pages: 12548-12561

We report a multifocal multiphoton time-correlated single photon counting (TCSPC) fluorescence lifetime imaging (FLIM) microscope system that uses a 16 channel multi-anode PMT detector. Multiphoton excitation minimizes out-of-focus photobleaching, multifocal excitation reduces non-linear in-plane photobleaching effects and TCSPC electronics provide photon-efficient detection of the fluorescence decay profile. TCSPC detection is less prone to bleaching- and movementinduced artefacts compared to wide-field time-gated or frequency-domain FLIM. This microscope is therefore capable of acquiring 3-D FLIM images at significantly increased speeds compared to single beam multiphoton microscopy and we demonstrate this with live cells expressing a GFP tagged protein. We also apply this system to time-lapse FLIM of NAD(P)H autofluorescence in single live cells and report measurements on the change in the fluorescence decay profile following the application of a known metabolic inhibitor.

JOURNAL ARTICLE

Poher V, Zhang HX, Kennedy GT, Griffin C, Oddos S, Gu E, Elson DS, Girkin JM, French PMW, Dawson MD, Neil MAAet al., 2007, Optical sectioning microscopes with no moving parts using a micro-stripe array light emitting diode, OPTICS EXPRESS, Vol: 15, Pages: 11196-11206, ISSN: 1094-4087

JOURNAL ARTICLE

Garcia DI, Lanigan P, Webb M, West TG, Requejo-Isidro J, Auksorius E, Dunsby C, Neil M, French P, Ferenczi MAet al., 2007, Fluorescence lifetime imaging to detect actomyosin states in mammalian muscle sarcomeres, BIOPHYSICAL JOURNAL, Vol: 93, Pages: 2091-2101, ISSN: 0006-3495

JOURNAL ARTICLE

Soloviev VY, McGinty J, Tahir KB, Neil MAA, Sardini A, Hajnal JV, Arridge SR, French PMWet al., 2007, Fluorescence lifetime tomography of live cells expressing enhanced green fluorescent protein embedded in a scattering medium exhibiting background autofluorescence, OPTICS LETTERS, Vol: 32, Pages: 2034-2036, ISSN: 0146-9592

JOURNAL ARTICLE

Soloviev VY, Tahir KB, McGinty J, Elson DS, Neil M, Sardini A, Hajnal J, Arridge SR, French PMWet al., 2007, Fluorescence lifetime imaging through turbid media reconstructed in the Fourier domain using time-gated imaging data, European Conference on Biomedical Optics, Publisher: SPIE

CONFERENCE PAPER

De Beule P, Owen DM, Manning HB, Talbot CB, Requejo-Isidro J, Dunsby C, McGinty J, Benninger RKP, Elson DS, Munro I, Lever MJ, Anand P, Neil MAA, French PMWet al., 2007, Rapid hyperspectral fluorescence lifetime imaging, MICROSCOPY RESEARCH AND TECHNIQUE, Vol: 70, Pages: 481-484, ISSN: 1059-910X

JOURNAL ARTICLE

Hegyi L, Talbot C, Monaco C, Sandison A, Davies AH, Peston D, Lever MJ, French PM, Stamp GWet al., 2007, Fluorescence lifetime imaging of unstained human atherosclerotic plaques., The 14th International Symposium on Atherosclerosis

CONFERENCE PAPER

Benninger RKP, Hofmann O, Onfelt B, Munro I, Dunsby C, Davis DM, Neil MAA, French PMW, de Mello AJet al., 2007, Fluorescence-lifetime imaging of DNA-Dye interactions within continuous-flow microfluidic systems (vol 46, pg 8525, 2007), ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 46, Pages: 8536-8536, ISSN: 1433-7851

JOURNAL ARTICLE

Poher V, Kennedy GT, Elson DS, French PMW, Neil AAAet al., 2007, Microscopy using micro-pixellated light emitting diodes, Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XIV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

CONFERENCE PAPER

Owen DM, Manning HB, de Beule P, Talbot C, Requejo-Isidro J, Dunsby C, McGinty J, Benninger RKP, Elson DS, Munro I, Galletly NP, Lever MJ, Stamp GW, Anand P, Neil MAA, French PMWet al., 2007, Development of a hyperspectral fluorescence lifetime imaging microscope and its application to tissue imaging, Conference on Imaging, Manipulation, and Analysis of Biomolecules, Cells, and Tissues V, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

CONFERENCE PAPER

Soloviev VY, Tahir KB, McGinty J, Elson DS, Neil M, Sardini A, Hajnal J, Arridge SR, French PMWet al., 2007, Fluorescence lifetime imaging through turbid media reconstructed in the Fourier domain using time-gated imaging data, Conference on Diffuse Optical Imaging of Tissue, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

CONFERENCE PAPER

Manning HB, Owen DM, Auksorius E, de Beule PAA, Oddos S, Talbot CB, Dunsby C, Munro I, Magee AI, Neil MAA, French PMWet al., 2007, Applications of rapid time-gated hyperspectral FLIM: live cell imaging of membrane order and 6-D microscopy, Conference on Confocal, Multiphoton and Nonlinear Microscopic Imaging III, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

CONFERENCE PAPER

Benninger RKP, Hofmann O, Onfelt B, Munro I, Dunsby C, Davis DM, Neil MAA, French PMW, de Mello AJet al., 2007, Fluorescence-lifetime imaging of DNA-dye interactions within continuous-flow microfluidic systems, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, Vol: 46, Pages: 2228-2231, ISSN: 1433-7851

JOURNAL ARTICLE

Ushakov DS, Konitsiotis A, Garcia DI, West TG, Auksorius E, Isidro JR, French PM, Ferenczi MAet al., 2007, Investigation of myosin essential light chain in skeletal muscle fibres by fluorescence lifetime imaging microscopy., 51st Annual Meeting of the Biophysical-Society, Publisher: BIOPHYSICAL SOCIETY, Pages: 296A-296A, ISSN: 0006-3495

CONFERENCE PAPER

Kennedy GT, Elson DS, Munro I, Poher V, French PMW, Neil MAAet al., 2007, Fluorescence lifetime imaging using light emitting diodes, Conference on Three-Dimensional and Multidimensional Microscopy - Image Acquisition and Processing XIV, Publisher: SPIE-INT SOC OPTICAL ENGINEERING, ISSN: 0277-786X

CONFERENCE PAPER

De Beule PAA, Dunsby C, Owen DM, Galletly NP, Anand U, Benham CD, Naylor A, Stamp GW, Anand P, French PMWet al., 2007, A novel hyperspectral lifetime probe for autofluorescence - art. no. 643303, Conference on Optical Fibers and Sensors for Medical Diagnostics and Treatment Applications VII, Pages: 43303-43303

The application of autofluorescence in non-invasive medical diagnostics could have great potential. Two major drawbacks inherent to this approach are low signal levels compared to those from exogenous fluorescent probes and complexity caused by the multiplicity of fluorescent biomolecules in tissue. Here we present a new optical system that is based on single channel detection via all optical fiber and call measure the fluorescence emission spectrum and fluorescence lifetime simultaneously for excitation wavelengths of 355 and 435nm. Single channel measurements integrate the signal normally available in all imaging setup and therefore have a better signal-to-noise ratio. Resolving both the fluorescence emission spectrum and fluorescence lifetime provides the opportunity to discriminate multiple fluorophores. This instrument is intended for NAD(P)H and flavin measurements for the dynamic monitoring of cellular metabolism and optical measurements of cancerous tissue. Initial results from a study of live cells and a clinical study of human skin lesions are presented.

CONFERENCE PAPER

Poher V, Kennedy G T, Elson D S, French, P M W, Neil, M A A, Zhang H, Gu E, Gong Z, Griffin C, Girkin J M, Dawson M Det al., 2007, Microscopy using micropixellated light emitting diodes, THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XIV, BIOS

CONFERENCE PAPER

Owen D M, Manning H B, de Beule P, Talbot C B, Requejo-Isidro J, Dunsby C W, McGinty J A, Benninger, R K P, Elson D S, Munro I H, Galletly N P, Lever J, Stamp G W, Anand P, Neil, M A A, French, P M Wet al., 2007, Rapid hyperspectral fluorescence lifetime imaging, IMAGING, MANIPULATION AND ANALYSIS OF BIOMOLECULES, CELLS, AND TISSUES V, BIOS, Publisher: SPIE

CONFERENCE PAPER

Onfelt B, Nedvetzki S, Benninger RKP, Purbhoo MA, Sowinski S, Hume AN, Seabra MC, Neil MAA, French PMW, Davis DMet al., 2006, Structurally distinct membrane nanotubes between human macrophages support long-distance vesicular traffic or surfing of bacteria, JOURNAL OF IMMUNOLOGY, Vol: 177, Pages: 8476-8483, ISSN: 0022-1767

JOURNAL ARTICLE

Lekadir, Karim, Elson, Daniel S, Requejo-Isidro, Jose, Dunsby, Christopher, McGinty, James, Galletly, Neil, Stamp, Gordon, French, Paul MW, Yang, Guang-Zhonget al., 2006, Tissue Characterization using Dimensionality Reduction and Fluorescence Imaging, Medical Image Computing and Computer Assisted Intervention, Publisher: Medical Image Computing and Computer Assisted Intervention

CONFERENCE PAPER

Treanor B, Lanigan PMP, Kumar S, Dunsby C, Munro I, Auksorius E, Culley FJ, Purbhoo MA, Phillips D, Neil MAA, Burshtyn DN, French PMW, Davis DMet al., 2006, Microclusters of inhibitory killer immunoglobulin like receptor signaling at natural killer cell immunological synapses, Journal of Cell Biology, Vol: 174, Pages: 153-161, ISSN: 1540-8140

We report the supramolecular organization of killer Ig–like receptor (KIR) phosphorylation using a technique applicable to imaging phosphorylation of any green fluorescent protein–tagged receptor at an intercellular contact or immune synapse. Specifically, we use fluorescence lifetime imaging (FLIM) to report Förster resonance energy transfer (FRET) between GFP-tagged KIR2DL1 and a Cy3-tagged generic anti-phosphotyrosine monoclonal antibody. Visualization of KIR phosphorylation in natural killer (NK) cells contacting target cells expressing cognate major histocompatibility complex class I proteins revealed that inhibitory signaling is spatially restricted to the immune synapse. This explains how NK cells respond appropriately when simultaneously surveying susceptible and resistant target cells. More surprising, phosphorylated KIR was confined to microclusters within the aggregate of KIR, contrary to an expected homogeneous distribution of KIR signaling across the immune synapse. Also, yellow fluorescent protein–tagged Lck, a kinase important for KIR phosphorylation, accumulated in a multifocal distribution at inhibitory synapses. Spatial confinement of receptor phosphorylation within the immune synapse may be critical to how activating and inhibitory signals are integrated in NK cells.

JOURNAL ARTICLE

Zhang HX, Gu E, Jeon CW, Gong Z, Dawson MD, Neil MAA, French PMWet al., 2006, Microstripe-array InGaN light-emitting diodes with individually addressable elements, IEEE PHOTONICS TECHNOLOGY LETTERS, Vol: 18, Pages: 1681-1683, ISSN: 1041-1135

JOURNAL ARTICLE

Owen DM, Lanigan PMP, Dunsby C, Munro I, Grant D, Neil MAA, French PMW, Magee AIet al., 2006, Fluorescence lifetime imaging provides enhanced contrast when imaging the phase-sensitive dye di-4-ANEPPDHQ in model membranes and live cells, BIOPHYSICAL JOURNAL, Vol: 90, Pages: L80-L82, ISSN: 0006-3495

JOURNAL ARTICLE

Galletly N, Mcginty JM, Cohen P, Munro I, Elson DS, Requejo-Isidro J, Dunsby C, Neil MA, Thillainayagam AV, French PM, Stamp GWet al., 2006, Fluorescence Lifetime Imaging for Detection of Gastrointestinal Cancer, GASTROINTESTINAL ENDOSCOPY, Vol: 63, Pages: AB250-AB250, ISSN: 0016-5107

JOURNAL ARTICLE

Galletly NP, McGinty JM, Cohen P, Munro I, Elson DS, Requejo-Isidro J, Dunsby C, Neil MAA, Thillainayagam AV, French PMW, Stamp GWet al., 2006, Detection of gastrointestinal malignancy by fluorescence lifetime imaging of UV laser induced tissue autofluorescence, Annual Meeting of the British-Society-of-Gastroenterology, Publisher: B M J PUBLISHING GROUP, Pages: A115-A115, ISSN: 0017-5749

CONFERENCE PAPER

Benninger RKP, Koc Y, Hofmann O, Requejo-Isidro J, Neil MAA, French PMW, deMello AJet al., 2006, Quantitative 3D mapping of fluidic temperatures within microchannel networks using fluorescence lifetime imaging, ANALYTICAL CHEMISTRY, Vol: 78, Pages: 2272-2278, ISSN: 0003-2700

JOURNAL ARTICLE

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