Imperial College London

ProfessorPaulFrench

Faculty of Natural SciencesDepartment of Physics

Professor of Physics and Vice Dean (Research) - FoNS
 
 
 
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Contact

 

+44 (0)20 7594 7706paul.french Website

 
 
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Assistant

 

Ms Judith Baylis +44 (0)20 7594 7713

 
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Location

 

609Blackett LaboratorySouth Kensington Campus

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Summary

 

Publications

Citation

BibTex format

@article{Gorlitz:2018:10.1002/jbio.201800087,
author = {Gorlitz, F and Guldbrand, S and Runcorn, T and Murray, R and Jaso-Tamame, A and Sinclair, H and Martinez-Perez, E and Taylor, J and Neil, M and Dunsby, CW and French, P},
doi = {10.1002/jbio.201800087},
journal = {Journal of Biophotonics},
title = {easySLM-STED: stimulated emission depletion microscopy with aberration correction, extended field of view and multiple beam scanning},
url = {http://dx.doi.org/10.1002/jbio.201800087},
year = {2018}
}

RIS format (EndNote, RefMan)

TY  - JOUR
AB - We demonstrate a simplified setup for STED microscopy with a straightforward alignment procedure that uses a single spatial light modulator (SLM) with collinear incident excitation and depletion beams to provide phase modulation of the beam profiles and correction of optical aberrations. We show that this approach can be used to extend the field of view for STED microscopy by correcting chromatic aberration that otherwise leads to walkoff between the focused excitation and depletion beams. We further show how this arrangement can be adapted to increase the imaging speed through multibeam excitation and depletion. Fine adjustments to the alignment can be accomplished using the SLM only, conferring the potential for automation.
AU - Gorlitz,F
AU - Guldbrand,S
AU - Runcorn,T
AU - Murray,R
AU - Jaso-Tamame,A
AU - Sinclair,H
AU - Martinez-Perez,E
AU - Taylor,J
AU - Neil,M
AU - Dunsby,CW
AU - French,P
DO - 10.1002/jbio.201800087
PY - 2018///
SN - 1864-063X
TI - easySLM-STED: stimulated emission depletion microscopy with aberration correction, extended field of view and multiple beam scanning
T2 - Journal of Biophotonics
UR - http://dx.doi.org/10.1002/jbio.201800087
UR - http://hdl.handle.net/10044/1/62063
ER -