Imperial College London


Faculty of Natural SciencesDepartment of Physics

Professor of Physics and Vice Dean (Research) - FoNS



+44 (0)20 7594 7706paul.french Website




Ms Judith Baylis +44 (0)20 7594 7713




609Blackett LaboratorySouth Kensington Campus






BibTex format

author = {Chennell, G and Willows, RJW and Warren, SC and Carling, D and French, PMW and Dunsby, C and Sardini, A},
doi = {10.3390/s16081312},
journal = {Sensors},
title = {Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM},
url = {},
volume = {16},
year = {2016}

RIS format (EndNote, RefMan)

AB - We describe an approach to non-invasively map spatiotemporal biochemical and physiological changes in 3D cell culture using Forster Resonance Energy Transfer (FRET) biosensors expressed in tumour spheroids. In particular, we present an improved Adenosine Monophosphate (AMP) Activated Protein Kinase (AMPK) FRET biosensor, mTurquoise2 AMPK Activity Reporter (T2AMPKAR), for fluorescence lifetime imaging (FLIM) readouts that we have evaluated in 2D and 3D cultures. Our results in 2D cell culture indicate that replacing the FRET donor, enhanced Cyan Fluorescent Protein (ECFP), in the original FRET biosensor, AMPK activity reporter (AMPKAR), with mTurquoise2 (mTq2FP), increases the dynamic range of the response to activation of AMPK, as demonstrated using the direct AMPK activator, 991. We demonstrated 3D FLIM of this T2AMPKAR FRET biosensor expressed in tumour spheroids using two-photon excitation.
AU - Chennell,G
AU - Willows,RJW
AU - Warren,SC
AU - Carling,D
AU - French,PMW
AU - Dunsby,C
AU - Sardini,A
DO - 10.3390/s16081312
PY - 2016///
SN - 1424-8239
TI - Imaging of Metabolic Status in 3D Cultures with an Improved AMPK FRET Biosensor for FLIM
T2 - Sensors
UR -
UR -
VL - 16
ER -